Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 702,544 C→A D70Y (GAC→TAC)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913702,5440CA100.0% 98.9 / NA 30D70Y (GAC→TAC) nagAN‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/15);  total (15/15)

CTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAG  >  NC_000913/702404‑702681
                                                                                                                                            |                                                                                                                                         
cTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACt                                                                                                                                   >  8:40038/1‑149 (MQ=255)
                  gCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGt                                                                                                                 >  2:33997/1‑149 (MQ=255)
                    ccTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGtt                                                                                                                >  6:63722/1‑148 (MQ=255)
                        tttCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTaa                                                                                                             <  5:63722/147‑1 (MQ=255)
                        tttCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTaa                                                                                                             <  3:46640/147‑1 (MQ=255)
                        tttCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAAc                                                                                                            <  1:33997/148‑1 (MQ=255)
                                    aTCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATa                                                                                                >  2:89473/1‑148 (MQ=255)
                                         tggtggTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGc                                                                                                                    >  6:80108/1‑123 (MQ=255)
                                         tggtggTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGc                                                                                                                    <  5:80108/123‑1 (MQ=255)
                                                                      gtACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAAc                                                              <  7:40038/148‑1 (MQ=255)
                                                                            ccTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACgttgtt                                                        >  3:117582/1‑148 (MQ=255)
                                                                            ccTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACgttgtt                                                        <  4:117582/148‑1 (MQ=255)
                                                                                        ttGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGCtc                                                   >  7:123567/1‑141 (MQ=255)
                                                                                        ttGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGAtc                                                   <  8:123567/141‑1 (MQ=255)
                                                                                             tttCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAAc                                                              <  8:93613/125‑1 (MQ=255)
                                                                                             tttCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAAc                                                              >  7:93613/1‑125 (MQ=255)
                                                                                             tttCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTc                                                       >  8:93094/1‑132 (MQ=255)
                                                                                             tttCTGCATGATTTCCAGCGTTTCCACGCTGACAGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTc                                                       <  7:93094/132‑1 (MQ=255)
                                                                                                cTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTgg                                              <  4:55280/138‑1 (MQ=255)
                                                                                                cTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTgg                                              >  3:55280/1‑138 (MQ=255)
                                                                                                  tcATGTTTTCCATGGTTTCCACGCTTTACGGTTTATGGGTTTATTTTAACTTTAAGGCGGCGGACGCGGTTAAATGGAAATCGCTTAAAACGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGc                                  <  2:837/147‑1 (MQ=255)
                                                                                                  gCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGc                                  >  5:30680/1‑148 (MQ=255)
                                                                                                        tttCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGa                           >  3:114966/1‑149 (MQ=255)
                                                                                                          tCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGAc                          <  1:89473/148‑1 (MQ=255)
                                                                                                            cAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGTATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCg                             >  7:70716/1‑143 (MQ=255)
                                                                                                            cAGCGTTTCCACGCTGACCGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGTATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCg                             <  8:70716/143‑1 (MQ=255)
                                                                                                                              cGCTTCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGAt                                                    >  4:30037/1‑102 (MQ=255)
                                                                                                                                 ttCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACt                                                                                                           >  6:139830/1‑44 (MQ=255)
                                                                                                                                 ttCAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACt                                                                                                           <  5:139830/44‑1 (MQ=255)
                                                                                                                                   cAGCGGTGTAGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAg  <  1:120343/147‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                         
CTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGTACAGCCTGATTTCTCATTGGCTTTCTGCATGATTTCCAGCGTTTCCACGCTGACCGCTTCAGCGGTGTCGTTAAACTGTACGCCGCCGCAGCCGTTTAACTGCACATCGATAAAACCGGGGGAGAGAATGGCCCCGTTCAGTGAACGTTGTTCGATCTCTGGCGGCAGTTCCGCTACCGGACAGACGCTTTTAATCAGGCCATCAG  >  NC_000913/702404‑702681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑