Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,159,109 A→G N414S (AAC→AGC)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,159,1090AG100.0% 111.1 / NA 32N414S (AAC→AGC) ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/17);  total (15/17)

ATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAA  >  NC_000913/1158964‑1159254
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aTTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCAt                                                                                                                                                <  1:49233/149‑1 (MQ=255)
          acacCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTc                                                                                                                                      >  1:65974/1‑149 (MQ=255)
                        gTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTAc                                                                                                                        <  2:1933/149‑1 (MQ=255)
                          gCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTAccc                                                                                                                      >  3:85216/1‑149 (MQ=255)
                              aTTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGt                                                                                                                    <  4:85216/147‑1 (MQ=255)
                                      aCTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCa                                                                                                          <  1:15139/149‑1 (MQ=255)
                                              tGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCt                                                                                                  >  7:15370/1‑149 (MQ=255)
                                                aaaaCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCt                                                                                                  <  2:65974/147‑1 (MQ=255)
                                                 aaaCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAt                                                                                               <  3:87423/149‑1 (MQ=255)
                                                                       gACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCAGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggccg                                                                         >  4:100042/1‑149 (MQ=255)
                                                                            aaGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAt                                                                                               >  4:56829/1‑122 (MQ=255)
                                                                            aaGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAt                                                                                               <  3:56829/122‑1 (MQ=255)
                                                                                 gCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAtgt                                                                                             >  4:13394/1‑119 (MQ=255)
                                                                                 gCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAtgt                                                                                             <  3:13394/119‑1 (MQ=255)
                                                                                             ggTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGc                                                   >  3:72795/1‑149 (MQ=255)
                                                                                             ggTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGc                                                   >  3:85251/1‑149 (MQ=255)
                                                                                              gTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGAc                                                                                                                                   <  6:7435/68‑1 (MQ=255)
                                                                                              gTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGAc                                                                                                                                   >  5:7435/1‑68 (MQ=255)
                                                                                                             cACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTgg                                                          >  5:125164/1‑126 (MQ=255)
                                                                                                             cACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTgg                                                          <  6:125164/126‑1 (MQ=255)
                                                                                                              aCCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTg                                         <  8:69221/142‑1 (MQ=255)
                                                                                                              aCCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTg                                         >  7:69221/1‑142 (MQ=255)
                                                                                                              aCCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTg                                  <  8:15370/149‑1 (MQ=255)
                                                                                                                         ttGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTg                                  <  1:113371/138‑1 (MQ=255)
                                                                                                                         ttGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTg                                  >  2:113371/1‑138 (MQ=255)
                                                                                                                             tgcATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGaaa                                                              >  5:32138/1‑106 (MQ=255)
                                                                                                                             tgcATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGaaa                                                              <  6:32138/106‑1 (MQ=255)
                                                                                                                                      tggtAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCgg                                                 <  4:79967/110‑1 (MQ=255)
                                                                                                                                      tggtAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCg          <  4:85251/149‑1 (MQ=255)
                                                                                                                                      tggtAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCg          <  4:72795/149‑1 (MQ=255)
                                                                                                                                           aaGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTAc      >  8:92849/1‑148 (MQ=255)
                                                                                                                                             gAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTa    >  2:117224/1‑148 (MQ=255)
                                                                                                                                              aaagCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTaaa  >  2:41741/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAA  >  NC_000913/1158964‑1159254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: