Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,022,710	G→T	G32V (GGC→GTC)	fliQ →	flagellar biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,022,710	0	G	T	100.0%	309.8 / NA	91	G32V (GGC→GTC)	fliQ	flagellar biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (48/43);  total (48/43)

GCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTT  >  NC_000913/2022564‑2022854                                                                                                                                                   |                                                                                                                                                 gcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTcc                                                                                                                                                <  5:18368/149‑1 (MQ=255)   aaTTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCtt                                                                                                                                              <  8:316779/149‑1 (MQ=255)   aaTTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCt                                                                                                                                               >  8:145881/1‑148 (MQ=255)        cTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGTGTTGGTCACGGTCCTTatcat                                                                                                                                         <  4:4161/149‑1 (MQ=255)         tGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTcc                                                                                                                                                >  8:306361/1‑141 (MQ=255)         tGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTcc                                                                                                                                                <  7:306361/141‑1 (MQ=255)         tGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTatcat                                                                                                                                         >  5:77503/1‑148 (MQ=255)          ggTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTatcat                                                                                                                                         >  8:62954/1‑147 (MQ=255)          ggTCGGTTCGCGGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAGGCGATGAAAGTCGCGCGGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTAtcatca                                                                                                                                       <  2:194892/149‑1 (MQ=255)           gTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTAtcatca                                                                                                                                       <  7:145881/148‑1 (MQ=255)           gTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTAtcatca                                                                                                                                       <  8:200118/148‑1 (MQ=255)             cgtggagCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGGCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTa                                                                                                                                    <  4:259960/143‑1 (MQ=255)                ttCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTAttt                                                                                                                                 <  2:56925/149‑1 (MQ=255)                ttCGCTGGCGCAGAGATTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTAttt                                                                                                                                 <  4:187808/149‑1 (MQ=255)                       gcgcAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTTGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGgc                                                                                                                          >  6:308337/1‑149 (MQ=255)                           agagCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGgccgc                                                                                                                       >  7:315768/1‑148 (MQ=255)                            gagCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCa                                                                                                                     >  1:310379/1‑149 (MQ=255)                                 tttACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACg                                                                                                                   >  4:169842/1‑146 (MQ=255)                                 tttACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACg                                                                                                                   >  3:283546/1‑146 (MQ=255)                                     cAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGAtt                                                                                                             <  7:66182/148‑1 (MQ=255)                                      aGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTaa                                                                                                           >  5:204547/1‑149 (MQ=255)                                       gCTAGTGATGCAAAATGTCACCTGTATCGGGCATGCTGCTGGGGACTGCAGCGCTGAAAGGGGCGGTGGCTCTGGGGGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAAc                                                                                                          <  2:139239/149‑1 (MQ=255)                                       gCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAAc                                                                                                          <  8:360484/149‑1 (MQ=255)                                         tAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACg                                                                                                         >  1:91576/1‑148 (MQ=255)                                           gagagGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGaaa                                                                                                      >  2:151089/1‑149 (MQ=255)                                                gCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGaaa                                                                                                      >  8:388047/1‑144 (MQ=255)                                                gCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGaaa                                                                                                      <  7:388047/144‑1 (MQ=255)                                                gCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACg                                                                                                 <  7:62954/149‑1 (MQ=255)                                                gCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACg                                                                                                 >  4:310526/1‑149 (MQ=255)                                                 cAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGc                                                                                                >  7:336736/1‑149 (MQ=255)                                                   aaaTGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTAtcatca                                                                                                                                       <  5:206319/108‑1 (MQ=255)                                                   aaaTGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTAtcatca                                                                                                                                       >  6:206319/1‑108 (MQ=255)                                                        acacCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGt                                                                                          <  2:310379/148‑1 (MQ=255)                                                          acCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTTTTGTTGGTAGCGTTGGGCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTa                                                                                       <  4:315345/149‑1 (MQ=255)                                                            cTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTAtt                                                                                     >  6:317187/1‑149 (MQ=255)                                                                    gtaatgaagaagGGGACTGAAGCGATGAAAGTCGCGCTCGCACTGGCTGCCCCTATAATGTATGTAAACTTGGTCAAGCTCATTATCATCAGTATTATGCAGTCCGCCACGCAGATTAACGAAAAGACGCTGTCGTTTATTAAGAAAat                                                                             >  8:165008/4‑149 (MQ=255)                                                                    gTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCAGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAat                                                                             >  7:255932/1‑149 (MQ=255)                                                                       atgatgatgGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAatca                                                                           <  7:308103/148‑1 (MQ=255)                                                                         gatgatgGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAgg                                                                                                                           >  1:293477/1‑98 (MQ=255)                                                                         gatgatgGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAgg                                                                                                                           >  1:288246/1‑98 (MQ=255)                                                                         gatgatgGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAgg                                                                                                                           <  2:288246/98‑1 (MQ=255)                                                                         gatgatgGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAgg                                                                                                                           <  2:293477/98‑1 (MQ=255)                                                                           tgatgGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGcc                                                                      <  3:169842/149‑1 (MQ=255)                                                                                     tGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCg                                                            >  5:34424/1‑149 (MQ=255)                                                                                     tGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCg                                                            >  2:13176/1‑149 (MQ=255)                                                                                       aaGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAGTGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGc                                                           >  3:356031/1‑148 (MQ=255)                                                                                       aaGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGc                                                           >  7:48740/1‑148 (MQ=255)                                                                                       aaGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGc                                                           >  7:120614/1‑148 (MQ=255)                                                                                       aaGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGc                                                           >  1:135804/1‑148 (MQ=255)                                                                                       aaGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGc                                                           >  6:299490/1‑148 (MQ=255)                                                                                       aaGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGc                                                           >  3:304875/1‑148 (MQ=255)                                                                                           gATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTGTTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCatta                                                      >  1:227615/1‑149 (MQ=255)                                                                                              gAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTAtt                                                                                     >  8:230384/1‑115 (MQ=255)                                                                                              gAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTAtt                                                                                     <  7:230384/115‑1 (MQ=255)                                                                                                aaGTCGCGCTGGCACTGGCTGCCACGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCattattatt                                                 <  6:77503/149‑1 (MQ=255)                                                                                                  gTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGc                                               <  3:310526/149‑1 (MQ=255)                                                                                                  gTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATAATTGc                                               >  2:79597/1‑149 (MQ=255)                                                                                                  gTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTACTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGc                                               <  1:151089/149‑1 (MQ=255)                                                                                                    cgcgCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCattattat                                                  >  8:348648/1‑144 (MQ=255)                                                                                                    cgcgCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCattattat                                                  <  7:348648/144‑1 (MQ=255)                                                                                                      cgcTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGa                                           >  5:52420/1‑149 (MQ=255)                                                                                                          ggCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGt                                       <  8:336736/149‑1 (MQ=255)                                                                                                            cACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTgg                                     <  6:204547/149‑1 (MQ=255)                                                                                                                ggCTGCCCCGCTATTGTTCGTAGCGATGGTCACGGTCCATATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGc                                 >  8:22820/1‑149 (MQ=255)                                                                                                                 gCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTGTTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCt                                <  2:227615/149‑1 (MQ=255)                                                                                                                 gCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGTCGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCt                                <  8:315768/149‑1 (MQ=255)                                                                                                                 gCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTa                                                                   >  5:83848/1‑114 (MQ=255)                                                                                                                 gCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTa                                                                   <  6:83848/114‑1 (MQ=255)                                                                                                                 gCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATg                                  <  5:317187/147‑1 (MQ=255)                                                                                                                 gCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCt                                >  8:193291/1‑149 (MQ=255)                                                                                                                      cccGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATc                           >  5:97541/1‑149 (MQ=255)                                                                                                                      cccGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATc                           >  2:245202/1‑149 (MQ=255)                                                                                                                      cccGCTATTGTTGGTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATc                           >  8:350202/1‑149 (MQ=255)                                                                                                                                   gTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAatcatc                                                                         <  8:296111/90‑1 (MQ=255)                                                                                                                                   gTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAatcatc                                                                         >  7:296111/1‑90 (MQ=255)                                                                                                                                   gTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGAtt               <  7:350202/148‑1 (MQ=255)                                                                                                                                   gTAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCAACGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTa              >  2:21882/1‑149 (MQ=255)                                                                                                                                    tAGCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTAc             <  6:34424/149‑1 (MQ=255)                                                                                                                                      gCGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGt           >  7:212494/1‑149 (MQ=255)                                                                                                                                       cGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTc          <  4:283546/149‑1 (MQ=255)                                                                                                                                       cGTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTc          >  7:324678/1‑149 (MQ=255)                                                                                                                                       cGTTGGTCACGGTCCGTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTc          >  5:43630/1‑149 (MQ=255)                                                                                                                                        gTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAAc                                                                                                          >  5:186938/1‑52 (MQ=255)                                                                                                                                        gTTGGTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAAc                                                                                                          <  6:186938/52‑1 (MQ=255)                                                                                                                                           ggTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGc       <  5:299490/148‑1 (MQ=255)                                                                                                                                           ggTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCa      >  6:283987/1‑149 (MQ=255)                                                                                                                                            gTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGtt                       <  3:76626/131‑1 (MQ=255)                                                                                                                                            gTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGtt                       >  4:76626/1‑131 (MQ=255)                                                                                                                                            gTCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCa      <  1:245202/148‑1 (MQ=255)                                                                                                                                             tCACGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGTATGCTCAATCTGTTGCTGGATTACGTCCGCAcc    <  1:21882/149‑1 (MQ=255)                                                                                                                                               aCGGTCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATAATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCtt  <  1:79597/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 GCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTT  >  NC_000913/2022564‑2022854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑