BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,284,655	T→A	Y83F (TAT→TTT)	actP ←	acetate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,284,655	0	T	A	98.9%	301.1 / NA	87	Y83F (TAT→TTT)	actP	acetate transporter
Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (42/44); total (42/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATC  >  NC_000913/4284513‑4284798

gcgcCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTccc                                                                                                                                           >  1:148034/1‑149 (MQ=255)

   ccccAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCgg                                                                                                                                        <  6:189760/149‑1 (MQ=255)

            aCGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGc                                                                                                                                 >  7:318613/1‑147 (MQ=255)

                aGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGccc                                                                                                                           >  2:97251/1‑149 (MQ=255)

                   cGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGtt                                                                                                                        <  2:197534/149‑1 (MQ=255)

                    gTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTc                                                                                                                       <  2:22358/149‑1 (MQ=255)

                     ttCGGCAATCAGGAACAAAATGATCGGCCAGCCCCCCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTGCt                                                                                                                      >  7:344634/1‑149 (MQ=255)

                        ggCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTACGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGa                                                                                                                   <  3:189438/149‑1 (MQ=255)

                         gccagcAGGCACAAACTGATCGCCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGaa                                                                                                                  <  2:267870/144‑1 (MQ=255)

                         gCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTgg                                                                                                                    >  6:287221/1‑147 (MQ=255)

                         gCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTgg                                                                                                                    >  8:16047/1‑147 (MQ=255)

                          cAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAg                                                                                                                 <  2:30695/149‑1 (MQ=255)

                            aTCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGc                                                                                                                                       >  4:113503/1‑125 (MQ=255)

                            aTCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGc                                                                                                                                       <  3:113503/125‑1 (MQ=255)

                            aTCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGcc                                                                                                               <  2:148438/149‑1 (MQ=255)

                               aGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAg                                                                                                             <  1:333259/148‑1 (MQ=255)

                                ggAACAAAATGATCGGCCAGCCCACCAGCAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCCGACATAAAGTCCCCGGCAAACGCCAGCCCGATCTGGAAGCCAGt                                                                                                            >  3:208092/1‑148 (MQ=255)

                                 gAACAAAATGATCGGCCAGCCCACCAGGAAGCCAATCGAGTAAATTAAGCCGTCATAGACGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATAAGGCGGCGGACATAAAGTCCCCGCCAAACGCCAGCCCGTACAGCAAGACAgaca                                                                                                          >  1:15314/1‑146 (MQ=255)

                                     aaaaTGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAAAACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATAtt                                                                                                      <  8:130907/149‑1 (MQ=255)

                                       aaTGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTgc                                                                                                    >  8:249900/1‑149 (MQ=255)

                                        aTGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATTAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTgcc                                                                                                   <  5:51712/149‑1 (MQ=255)

                                        aTGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTgcc                                                                                                   <  1:388085/149‑1 (MQ=255)

                                           aTCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTgccgc                                                                                                 <  8:318613/148‑1 (MQ=255)

                                                ccAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCgg                                                                                           >  8:170267/1‑149 (MQ=255)

                                                    cccACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCTGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCTGGCAATCTCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTgta                                                                                       <  8:237743/149‑1 (MQ=255)

                                                     ccaccaGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTgtag                                                                                      <  4:1020/149‑1 (MQ=255)

                                                      ccccaGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACCCCAGCGCGGAGCTCCCCAAGAATGCGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGATCTGGAAGCGAGGGATATTGCCGCCTGCGGAgtag                                                                                      >  4:183611/3‑148 (MQ=255)

                                                      caccaGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTgtag                                                                                      >  1:341102/1‑148 (MQ=255)

                                                       accaGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCGGTGATATTGCCGCCTGCGGTgtag                                                                                      >  8:276256/1‑147 (MQ=255)

                                                       accaGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTgtag                                                                                      >  6:137141/1‑147 (MQ=255)

                                                       accaGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTgtag                                                                                      >  6:7732/1‑147 (MQ=255)

                                                         caGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTagtagt                                                                                  <  2:78146/149‑1 (MQ=255)

                                                         caGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGACGGATGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGTCGGACATAAAGTCCCCGGCAATCGCCAGACCGTTCTGGAAGCCATTGATATTGCCGCCTGCGGTGTagtagt                                                                                  <  5:364725/149‑1 (MQ=255)

                                                           ggAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGtt                                                                                                                        >  5:80036/1‑109 (MQ=255)

                                                           ggAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGtt                                                                                                                        <  6:80036/109‑1 (MQ=255)

                                                               gCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTAc                                                                            <  2:148034/149‑1 (MQ=255)

                                                                  cAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTAc                                                                            >  8:1378/1‑146 (MQ=255)

                                                                    gCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGaa                                                                       >  6:40778/1‑149 (MQ=255)

                                                                     cGAGTAAATTAAGCCGTCATAGCGTGATTTAAACTCCAGCTCGGAGATCCCCTAGAATTAGGGGGCGGACTTTTATTCCCCTGCAATCGCCAGCCGGTTGTGGAAGCCAGTGATATTGCCGCCTGCTGTGTAGTAGTCGCTACgagatc                                                                      <  2:15314/149‑3 (MQ=255)

                                                                      gAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACg                                                                     <  1:97251/149‑1 (MQ=255)

                                                                      gAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTCCCGCCTGCGGTGTAGTAGTCGCTACGAGAACg                                                                     >  1:226334/1‑149 (MQ=255)

                                                                       aGTAAATTAATCCGTCATAGCCGGTGGTAAACACCAGCGCGGAGAGCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGt                                                                    <  2:226334/149‑1 (MQ=255)

                                                                        gTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTa                                                                   >  4:315080/1‑149 (MQ=255)

                                                                         tAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTAc                                                                  <  5:287221/149‑1 (MQ=255)

                                                                             ttAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGt                                                              >  4:359195/1‑149 (MQ=255)

                                                                               aaGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCg                                                                                            >  4:234250/1‑117 (MQ=255)

                                                                               aaGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCg                                                                                            <  3:234250/117‑1 (MQ=255)

                                                                               aaGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGACGCTACGAGAACGTACGCGtt                                                             <  2:341102/148‑1 (MQ=255)

                                                                                aGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGtttt                                                           <  1:240545/149‑1 (MQ=255)

                                                                                 gCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTg                                                          <  3:359195/149‑1 (MQ=255)

                                                                                  ccGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGa                                                         >  2:73565/1‑149 (MQ=255)

                                                                                        tAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGcc                                                     >  4:270649/1‑147 (MQ=255)

                                                                                        tAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGcc                                                     >  1:321442/1‑147 (MQ=255)

                                                                                        tAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCa                                                   <  7:1378/149‑1 (MQ=255)

                                                                                          gCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGt                                                 <  1:241791/149‑1 (MQ=255)

                                                                                           ccGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGgtg                                                                                         <  6:102599/108‑1 (MQ=255)

                                                                                           ccGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGgtg                                                                                         >  5:102599/1‑108 (MQ=255)

                                                                                            cGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAg                                               >  4:224852/1‑149 (MQ=255)

                                                                                            cGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGACCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAg                                               >  4:229705/1‑149 (MQ=255)

                                                                                              gAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCACGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGt                                             <  2:50702/149‑1 (MQ=255)

                                                                                                ggTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAAc                                                                      <  3:355581/122‑1 (MQ=255)

                                                                                                ggTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAAc                                                                      >  4:355581/1‑122 (MQ=255)

                                                                                                ggTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTaa                                           <  5:7732/149‑1 (MQ=255)

                                                                                                ggTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTaa                                           <  7:16047/149‑1 (MQ=255)

                                                                                                 gTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAAt                                          <  5:40778/149‑1 (MQ=255)

                                                                                                   aaaCACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGc                                        >  4:306999/1‑149 (MQ=255)

                                                                                                      cacCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGa                                     <  1:21178/149‑1 (MQ=255)

                                                                                                           gcgcGGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTa                                <  5:137141/149‑1 (MQ=255)

                                                                                                            cgcgGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTa                                >  2:141009/1‑148 (MQ=255)

                                                                                                              cgGAGATCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAac                             <  2:60767/149‑1 (MQ=255)

                                                                                                                gagaTCCCCAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAacac                           <  7:170267/149‑1 (MQ=255)

                                                                                                                      cccAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACAcgacga                      >  7:373800/1‑148 (MQ=255)

                                                                                                                      cccAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACAcgacga                      >  2:208596/1‑148 (MQ=255)

                                                                                                                       ccAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGaa                     >  1:2970/1‑148 (MQ=255)

                                                                                                                        cAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGaaaa                   >  8:9423/1‑149 (MQ=255)

                                                                                                                        cAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGaaaa                   >  5:159231/1‑149 (MQ=255)

                                                                                                                        cAAGAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGaaa                    >  6:239081/1‑148 (MQ=255)

                                                                                                                           gAATGAGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAAt                  >  6:298324/1‑147 (MQ=255)

                                                                                                                              tGAGGCGGCGTACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGa             <  6:174064/149‑1 (MQ=255)

                                                                                                                                aGGCGGCGGACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAgg                                              >  6:104530/1‑114 (MQ=255)

                                                                                                                                   cggcggACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACata        <  1:141009/149‑1 (MQ=255)

                                                                                                                                    ggcggACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGcc                                       >  5:174122/1‑117 (MQ=255)

                                                                                                                                    ggcggACATAAAGTCCCCGGCAAGCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGcc                                       <  6:174122/117‑1 (MQ=255)

                                                                                                                                      cggACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACataata     <  1:360561/149‑1 (MQ=255)

                                                                                                                                       tgACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAACCCAGTGATATTTCCGCCAGCGGAGTAGTAGTCGCTACGAGAACGTACGCCTTTTTAt                                                        >  4:182871/2‑97 (MQ=255)

                                                                                                                                       ggACATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGAt                                                        <  3:182871/97‑1 (MQ=255)

                                                                                                                                         aCATAAAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATc  <  6:159231/149‑1 (MQ=255)

GCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATC  >  NC_000913/4284513‑4284798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`