| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,629,495 | 0 | A | T | 23.1% | 46.8 / 11.4 | 26 | L15Q (CTG→CAG) | rbbA | ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (10/10); new base T (3/3); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTT > NC_000913/3629355‑3629621 | gggCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGTTCCCGTCCCGCCTGTcgcg > 3:37683/1‑148 (MQ=255) cGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGTTCCCGTCCCGCCTGTCGCGCAACTgg < 4:37683/149‑1 (MQ=255) gAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCTGTCGCGCAACTGGcgg > 3:10016/1‑149 (MQ=255) aaTCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCTGTCGCGCAACTGGcgggc < 4:10016/149‑2 (MQ=255) caaGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGtt > 6:5120/1‑149 (MQ=255) cTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCTGTCGCGCAACTGGcgggcgt > 6:27698/1‑132 (MQ=255) cTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCTGTCGCGCAACTGGcgggcgt < 5:27698/135‑4 (MQ=255) cGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGAt < 3:70975/149‑1 (MQ=255) gACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGc < 4:30417/126‑1 (MQ=255) gACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGc > 3:30417/1‑126 (MQ=255) gACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGa < 5:5120/147‑1 (MQ=255) gACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATg > 8:1224/1‑149 (MQ=255) aCGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGc > 1:6523/1‑149 (MQ=255) gCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcac < 7:1224/149‑1 (MQ=255) aTACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAgg > 5:108818/1‑140 (MQ=255) aTACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAgg < 6:108818/140‑1 (MQ=255) gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAcc > 2:59721/1‑105 (MQ=255) gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAcc < 1:59721/105‑1 (MQ=255) tatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAccc < 2:6523/149‑1 (MQ=255) gcAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGaa < 5:21464/117‑1 (MQ=255) gcAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGaa > 6:21464/1‑117 (MQ=255) aaCGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcgggacggga > 7:77594/1‑58 (MQ=255) aaCGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGcgggacggga < 8:77594/58‑1 (MQ=255) aaCGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCgg > 1:114701/1‑149 (MQ=255) cccgccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGtttt > 7:59543/1‑133 (MQ=255) cccgccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGtttt < 8:59543/133‑1 (MQ=255) | GGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACG‑CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGCCCGGCAAACCGGTTTT > NC_000913/3629355‑3629621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |