Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	963,764	0	G	A	28.1%	43.5 / 19.4	32	intergenic (+96/‑64)	rpsA/ihfB	30S ribosomal subunit protein S1/integration host factor (IHF), DNA‑binding protein, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (12/11);  new base A (3/6);  total (15/17)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CAACGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGAAGATG  >  NC_000913/963626‑963900                                                                                                                                           |                                                                                                                                         caacGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCCGTAGCCGCCCTTa                                                                                                                                <  1:55746/149‑1 (MQ=255)       aaTGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCATTAAGGGCGGCCGTAGCCGCCCt                                                                                                                                  <  6:167555/141‑1 (MQ=255)       aaTGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCATTAAGGGCGGCCGTAGCCGCCCt                                                                                                                                  >  5:167555/1‑141 (MQ=255)            cTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTAGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATgaag                                                                                                                      >  1:211475/1‑148 (MQ=255)              gAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCaa                                                                                                                   >  5:69033/1‑149 (MQ=255)                     tCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAAcagca                                                                                                              <  6:69033/147‑1 (MQ=255)                         agcagcTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCt                                                                                                        >  2:152302/1‑149 (MQ=255)                                    gCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCtt                                                                                             >  5:35211/1‑149 (MQ=255)                                            ttCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCCGTAGCCGCCCTTagtgct                                                                                                                           <  6:33114/111‑6 (MQ=38)                                                       ttCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCa                                                                          >  2:124787/1‑149 (MQ=255)                                                          gggATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCCGTAGCCGCCCTTa                                                                                                                                >  6:46835/1‑92 (MQ=39)                                                          gggATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCCGTAGCCGCCCTTa                                                                                                                                <  5:46835/92‑1 (MQ=39)                                                                 ttATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGt                                                                 <  6:35211/148‑1 (MQ=255)                                                                       cGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAAtt                                                          >  1:139842/1‑149 (MQ=255)                                                                           gTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATc                                                                           >  6:111329/1‑128 (MQ=255)                                                                           gTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATa                                                      <  1:124787/149‑1 (MQ=255)                                                                           gTTTGTTGAGTTTACTTGACAGATTGCACGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATc                                                                           <  5:111329/128‑1 (MQ=255)                                                                              tgttgAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGa                                                    <  1:152302/148‑1 (MQ=255)                                                                                ttgAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGaa                                                            >  5:44908/1‑138 (MQ=255)                                                                                ttgAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGaa                                                            <  6:44908/138‑1 (MQ=255)                                                                                                                     aTCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACg            >  8:8480/1‑149 (MQ=255)                                                                                                                     aTCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACg            >  5:91638/1‑149 (MQ=255)                                                                                                                      tCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAAc                                                                                     >  4:69387/1‑75 (MQ=255)                                                                                                                      tCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAAc                                                                                     <  3:69387/75‑1 (MQ=255)                                                                                                                        aaGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGtt         <  6:91638/149‑1 (MQ=255)                                                                                                                           gattAAGGGCGGCCGTAGCCGCCCTTAGTCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCa                                     >  3:153890/4‑118 (MQ=38)                                                                                                                           gattAAGGGCGGCCGTAGCCGCCCTTAGTCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCa                                     <  4:153890/115‑1 (MQ=38)                                                                                                                           gattAAGGGCGGCCGTAGCCGCCCTTAGTCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGa       <  7:32485/145‑1 (MQ=255)                                                                                                                            aCTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGAAg     <  2:139842/149‑1 (MQ=255)                                                                                                                                aGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGAAGATg  <  7:8480/148‑1 (MQ=255)                                                                                                                                          cGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGa                                                        <  3:51379/84‑1 (MQ=255)                                                                                                                                          cGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGa                                                        >  4:51379/1‑84 (MQ=255)                                                                                                                                           |                                                                                                                                         CAACGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGAAGATG  >  NC_000913/963626‑963900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑