Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,525,613	A→G	5.8%	I123T (ATT→ACT)	ansP ←	L‑asparagine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,525,613	0	A	G	5.8%	203.8 / 3.0	69	I123T (ATT→ACT)	ansP	L‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base A (34/30);  new base G (2/2);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

CCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAAC  >  NC_000913/1525465‑1525759                                                                                                                                                     |                                                                                                                                                   ccAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATcaacaa                                                                                                                                                    <  7:335099/149‑1 (MQ=255)   aGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATc                                                                                                                                                  <  6:94250/149‑1 (MQ=255)        tCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGt                                                                                                                                             >  7:252122/1‑149 (MQ=255)            tctcCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGCGCGCAAAGCCCCACTGCGGCACGCCGCCAAACACACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATc                                                                                                                                         >  2:295013/1‑149 (MQ=255)                cggaaaCCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTTCGGCACGCAGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGc                                                                                                                                       <  8:25429/144‑1 (MQ=255)                 gCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCa                                                                                                                                    <  4:6340/149‑1 (MQ=255)                  cAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAg                                                                                                                                   <  8:69066/149‑1 (MQ=255)                  cAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCCAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAg                                                                                                                                   <  3:35496/149‑1 (MQ=255)                     aCCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGtt                                                                                                                                 >  8:299730/1‑148 (MQ=255)                     aCCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACCCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGtt                                                                                                                                 >  6:341701/1‑148 (MQ=255)                       cATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTtgat                                                                                                                              <  2:141525/149‑1 (MQ=255)                       cATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTtgat                                                                                                                              <  4:87527/149‑1 (MQ=255)                            cacaCCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAg                                                                                                                          <  1:200628/148‑1 (MQ=255)                            cacaCCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAg                                                                                                                          <  6:409767/148‑1 (MQ=255)                               acCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACa                                                                                                                      >  8:375891/1‑149 (MQ=255)                                  gATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATc                                                                                                                    >  4:94323/1‑148 (MQ=255)                                   aTCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATcca                                                                                                                  <  3:94323/149‑1 (MQ=255)                                    tCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGCCCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATccag                                                                                                                 >  6:372301/1‑149 (MQ=255)                                      atatTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAGTCCCCGTCATCGCCCAGTTGATGAAGTACATccagc                                                                                                                >  7:27395/1‑148 (MQ=255)                                      atatTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAGTCCCCGTCATCGCCCAGTTGATGAAGTACATccagc                                                                                                                >  7:34145/1‑148 (MQ=255)                                       tatTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATccagc                                                                                                                >  6:185168/1‑147 (MQ=255)                                       tatTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATccagc                                                                                                                >  7:45113/1‑147 (MQ=255)                                            aTGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCa                                                                                                           <  5:207844/147‑1 (MQ=255)                                            aTGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCa                                                                                                           >  6:201877/1‑147 (MQ=255)                                            aTGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCa                                                                                                           >  6:207844/1‑147 (MQ=255)                                            aTGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCa                                                                                                           <  5:201877/147‑1 (MQ=255)                                            aTGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCAACTGCGTCACGCCGCCAAACGCACACCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCaa                                                                                                          >  6:298662/1‑148 (MQ=255)                                                  ccAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAg                                                                                                    <  7:299730/148‑1 (MQ=255)                                                   cAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCg                                                                                                  <  8:45113/149‑1 (MQ=255)                                                   cAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCAGCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCg                                                                                                  >  5:352440/1‑149 (MQ=255)                                                      cGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTATTGCATATACAGAGCGCCGGCGGTAATATCAACAATCCCCGGCAGCGCCCAGTTGATGAAGTACAGCCAGCCCGCACCATAAGCGGCt                                                                                               >  2:174662/1‑149 (MQ=255)                                                              aGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCAcc                                                                                       >  1:114829/1‑149 (MQ=255)                                                              aGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCAc                                                                                        <  4:373857/148‑1 (MQ=255)                                                                    gcgagcgCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCaaaaa                                                                                 <  5:185168/149‑1 (MQ=255)                                                                           cAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAGTCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACgg                                                                          <  8:27395/149‑1 (MQ=255)                                                                           cAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAGTCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACgg                                                                          <  8:34145/149‑1 (MQ=255)                                                                            aaaGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACggg                                                                         >  1:372704/1‑149 (MQ=255)                                                                                 cccACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGAAATATCAACTATCCCCGTCATCGCCCAGTTGATGAAGTACTTCCACCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATaa                                                                    <  2:340248/149‑1 (MQ=255)                                                                                     cTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGaaa                                                                >  6:252999/1‑149 (MQ=255)                                                                                     cTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGaaa                                                                >  5:77847/1‑149 (MQ=255)                                                                                        cGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGt                                                                                                                                  <  6:235875/80‑1 (MQ=255)                                                                                        cGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGt                                                                                                                                  >  5:235875/1‑80 (MQ=255)                                                                                        cGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGGAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACa                                                              >  3:285247/1‑148 (MQ=255)                                                                                        cGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACaa                                                             <  6:77847/149‑1 (MQ=255)                                                                                        cGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACa                                                              >  8:249360/1‑148 (MQ=255)                                                                                          gCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACACTCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACaaaa                                                           >  5:301041/1‑149 (MQ=255)                                                                                          gCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACaaaa                                                           <  5:252999/149‑1 (MQ=255)                                                                                          gCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACaaaa                                                           <  2:372704/149‑1 (MQ=255)                                                                                            aCGCCGCCAAACGAACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCAAGAAGATCAAGGACATCCAGCCAGCAACAAAAGCGGCTTTGTCACCCAAAAACTCACGGGCATAAGAAACAAAACt                                                         >  1:287181/1‑149 (MQ=255)                                                                                             cgccgcCAAACACACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTg                                                        <  1:295013/149‑1 (MQ=255)                                                                                              gccgccAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCACCATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGc                                                       >  1:43331/1‑149 (MQ=255)                                                                                                cgccAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCa                                                     >  5:235091/1‑149 (MQ=255)                                                                                                   cAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTg                                                  <  5:341701/149‑1 (MQ=255)                                                                                                      aCGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGaa                                               <  8:252122/149‑1 (MQ=255)                                                                                                            cccAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCgg                                         >  2:294955/1‑149 (MQ=255)                                                                                                            cccAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCgg                                         >  2:55471/1‑149 (MQ=255)                                                                                                               cgTAAAGCCTATACAGCGCGCCGGCGGAAATCTCAACCAGCCCCGTCATCGCCCAGTACACGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGgtgt                                      <  5:303321/148‑1 (MQ=255)                                                                                                               aGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGgtgt                                      <  6:235091/149‑1 (MQ=255)                                                                                                                       atatACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACg                                                                           <  6:337916/104‑1 (MQ=255)                                                                                                                       atatACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACg                                                                           >  5:337916/1‑104 (MQ=255)                                                                                                                       atatACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAg                              <  7:375891/149‑1 (MQ=255)                                                                                                                       atatACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAg                              >  8:60906/1‑149 (MQ=255)                                                                                                                        tataCAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGc                             >  2:326231/1‑149 (MQ=255)                                                                                                                           aCAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCt                            <  4:24433/147‑1 (MQ=255)                                                                                                                           aCAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCt                            >  3:24433/1‑147 (MQ=255)                                                                                                                             agagCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCAcc                        >  7:344102/1‑149 (MQ=255)                                                                                                                                 cGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAAt                    <  5:372301/149‑1 (MQ=255)                                                                                                                                     ggcggTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCAc                >  8:185458/1‑149 (MQ=255)                                                                                                                                             tatCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAAt         >  7:194378/1‑148 (MQ=255)                                                                                                                                              atCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATa        >  1:235043/1‑148 (MQ=255)                                                                                                                                                caacaaTCCCCGTCATCGCCCAGTTGATGAATTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATaaaa     <  6:304495/149‑1 (MQ=255)                                                                                                                                                  acaaTCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGACACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATaaaaaa   <  5:298662/149‑1 (MQ=255)                                                                                                                                                    aaTCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAAc  >  4:326293/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CCAGAACTCCATCTCCGCAAACCATTTCACACCGATCATATTCATGGTGCCAACGATGGTAAGTGCAGCGAGCGCAAAGACCCACTGCGGCACGCCGCCAAACGCACCCCAGTAATGCATATACAGAGCGACGGCGGTAATATCAACAATCCCCGTCATCGCCCAGTTGATGAAGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAAC  >  NC_000913/1525465‑1525759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑