Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,394,953 G→A 5.9% P296P (CCG→CCA)  nnr → bifunctional NAD(P)H‑hydrate repair enzyme; C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,394,9530GA 5.9% 217.3 / 2.4 68P296P (CCG→CCA) nnrbifunctional NAD(P)H‑hydrate repair enzyme; C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase
Reads supporting (aligned to +/- strand):  ref base G (31/33);  new base A (2/2);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01

ACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAG  >  NC_000913/4394807‑4395097
                                                                                                                                                  |                                                                                                                                                
aCTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTCCTGACCCGCAGTGAAAACATTGCGCCgct                                                                                                                                                >  4:127683/1‑149 (MQ=255)
     gCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGa                                                                                                                                           <  8:70544/149‑1 (MQ=255)
        tAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGGGCTGGTTTAGTCAGAGTACTGACCCGCAGTGAAGACCTTGCGCCGCTGCTGACTg                                                                                                                                        >  5:150035/1‑149 (MQ=255)
         aaaGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTg                                                                                                                                        >  8:134734/1‑148 (MQ=255)
           aGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCAc                                                                                                                                     <  6:139010/149‑1 (MQ=255)
                         ggcTGGTACTTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTACGCCGGGGGCAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATg                                                                                                                       <  3:73870/149‑1 (MQ=255)
                         ggcTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATg                                                                                                                       <  7:109505/149‑1 (MQ=255)
                         ggcTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATg                                                                                                                       <  6:196084/149‑1 (MQ=255)
                         ggcTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATg                                                                                                                       >  8:163641/1‑149 (MQ=255)
                          gcTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGcgccgc                                                                                                                                                 >  7:25821/1‑122 (MQ=255)
                          gcTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGcgccgc                                                                                                                                                 <  8:25821/122‑1 (MQ=255)
                            ttgTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTTACTGCACCACCGGAATTGATGGTg                                                                                                                    >  6:92727/3‑149 (MQ=255)
                              gTAATTATCGGGGGCGAGCACGGCACGGCGGGGGCTATTCGCATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTg                                                                                                                    <  2:91776/147‑1 (MQ=255)
                               gaaTTATCGGTGGCGATCACGGCACGGCGTGGGCTATTCGTATCACGGGGGAAGCGGTGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCAt                                                                                                                 <  6:342843/148‑1 (MQ=255)
                                    aTCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAAc                                                                                                             <  8:76947/148‑1 (MQ=255)
                                       cgTGGAGATCACGGCACGGCTGGGGCTATTCGTATGACGGGGCAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGa                                                                                                          <  6:96643/147‑1 (MQ=255)
                                         tGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGGTGGTGCATGAACTGACGa                                                                                                       >  6:17327/1‑149 (MQ=255)
                                         tGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGa                                                                                                       <  2:12167/149‑1 (MQ=255)
                                         tGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGa                                                                                                       >  7:308956/1‑149 (MQ=255)
                                          ggCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTTACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGAt                                                                                                      >  4:278498/1‑149 (MQ=255)
                                              cccccggcaaggcGGGGCCGCTCCGTCTCACGGGGGAAGCGGCGCTGCGGGCTGGTGCTGGGTTAGGCCGCGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGAc                                                                                                  <  2:389975/145‑1 (MQ=255)
                                              aTCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGAc                                                                                                  >  5:68165/1‑149 (MQ=255)
                                                      ccggcggTGGCTATGCGTATGCAGGCGGCAGCGGCTCTGCGTGCTGGTGCTGGGTTAGTCCGAGTACTGACCCGCAGTGAAGACATTGCGCAGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTAc                                                                                          <  6:150035/148‑1 (MQ=255)
                                                      ccggcggGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTAc                                                                                          <  6:68165/148‑1 (MQ=255)
                                                      acggcGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTAc                                                                                          <  7:195549/149‑1 (MQ=255)
                                                       cggcggGGGCTATTCGTATGCCGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTAcc                                                                                         <  3:269848/149‑1 (MQ=255)
                                                       cggcggGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTAcc                                                                                         <  8:308956/149‑1 (MQ=255)
                                                               gCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCt                                                                                 >  6:15884/1‑149 (MQ=255)
                                                                cTATTCGTATGACGGGGTAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGTCTGCACTACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTg                                                                                <  5:92727/149‑1 (MQ=255)
                                                                 tATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTAc                                                                                          >  6:52453/1‑138 (MQ=255)
                                                                 tATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTAc                                                                                          <  5:52453/138‑1 (MQ=255)
                                                                  aTTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCAGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTgg                                                                               >  7:140929/1‑148 (MQ=255)
                                                                   ttCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGaa                                                                             >  8:301380/1‑149 (MQ=255)
                                                                      gTATGCCGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATgg                                                                          <  7:134734/149‑1 (MQ=255)
                                                                      gTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATgg                                                                          <  2:401266/149‑1 (MQ=255)
                                                                            cGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGAt                                                                    <  7:163641/149‑1 (MQ=255)
                                                                                 gAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAAt                                                                                                                            >  6:321164/1‑88 (MQ=255)
                                                                                 gAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAAt                                                                                                                            <  5:321164/88‑1 (MQ=255)
                                                                                  aaGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGGTGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATgtggtg                                                              <  5:17327/149‑1 (MQ=255)
                                                                                  aaGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATgtggtg                                                              <  5:15884/149‑1 (MQ=255)
                                                                                   aGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGAct                                                                                                 <  7:44753/113‑1 (MQ=255)
                                                                                   aGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGAct                                                                                                 >  8:44753/1‑113 (MQ=255)
                                                                                          cTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAAAATTGCGCCGCTGCGGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATCGACTCTCTTACCGAATGCCTGGAATGGGCCGATGTGGTGGTGATTgg                                                      <  4:226480/149‑1 (MQ=255)
                                                                                               tgctggtgctggTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCg                                                 >  4:113134/1‑149 (MQ=255)
                                                                                                  tggtgctggtTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGCCTGCACGACCGGAATTGATGGTGCATGAACTGATGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTc                                              >  6:335083/1‑149 (MQ=255)
                                                                                                        tggtTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGc                                         >  7:271009/1‑148 (MQ=255)
                                                                                                         ggtTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGc                                         >  1:361649/1‑147 (MQ=255)
                                                                                                            ttAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGc                                     >  8:111175/1‑148 (MQ=255)
                                                                                                               gTCCGAGTACTGACCCGCAGTGAATACATTGGGCCGCTGCTGACTTCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGTAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAaga                                 <  4:83744/149‑1 (MQ=255)
                                                                                                               gTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCaa                                   >  5:271752/1‑147 (MQ=255)
                                                                                                                   gAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATgtggtg                                                              >  5:300508/1‑116 (MQ=255)
                                                                                                                   gAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATgtggtg                                                              <  6:300508/116‑1 (MQ=255)
                                                                                                                   gAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgg                             <  4:176713/149‑1 (MQ=255)
                                                                                                                      tACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTggg                            >  8:45745/1‑147 (MQ=255)
                                                                                                                      tACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTggg                            <  7:45745/147‑1 (MQ=255)
                                                                                                                         tGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgg                             <  8:60424/143‑1 (MQ=255)
                                                                                                                         tGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgg                             >  7:60424/1‑143 (MQ=255)
                                                                                                                         tGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGaa                        >  8:225390/1‑148 (MQ=255)
                                                                                                                           aCCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGaaaa                      >  3:162375/1‑148 (MQ=255)
                                                                                                                           aCCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGaaaa                      >  4:399330/1‑148 (MQ=255)
                                                                                                                               gCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAga                  >  6:23336/1‑147 (MQ=255)
                                                                                                                                    gAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgg                             >  4:200032/1‑132 (MQ=255)
                                                                                                                                    gAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgg                             <  3:200032/132‑1 (MQ=255)
                                                                                                                                     aaaaCATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTggg                            >  3:160306/1‑132 (MQ=255)
                                                                                                                                     aaaaCATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTggg                            <  4:160306/132‑1 (MQ=255)
                                                                                                                                     aaaaCATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCa            >  3:167744/1‑148 (MQ=255)
                                                                                                                                     aaaaCATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGGCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCa            >  6:123150/1‑148 (MQ=255)
                                                                                                                                       aaCATTGCGCCACTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGa                                                          >  8:286874/1‑100 (MQ=255)
                                                                                                                                       aaCATTGCGCCACTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGa                                                          >  8:293632/1‑100 (MQ=255)
                                                                                                                                       aaCATTGCGCCACTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGa                                                          <  7:286874/100‑1 (MQ=255)
                                                                                                                                       aaCATTGCGCCACTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGa                                                          <  7:293632/100‑1 (MQ=255)
                                                                                                                                              cgccgcTGCTGACTTCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTga   <  3:278498/148‑1 (MQ=255)
                                                                                                                                              cgccgcTGCTGACTGCACGACCGGAATTGATGGTGCATGATCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGtt     >  5:266461/1‑146 (MQ=255)
                                                                                                                                              cgccgcTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTga   <  7:345353/148‑1 (MQ=255)
                                                                                                                                              cgccgcTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTga   <  8:271009/148‑1 (MQ=255)
                                                                                                                                              cgccgcTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTga   <  7:111175/148‑1 (MQ=255)
                                                                                                                                               gccgcTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTgag  >  3:375986/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ACTTCGCATAAAGGCGATCACGGGCGGCTGGTAATTATCGGTGGCGATCACGGCACGGCGGGGGCTATTCGTATGACGGGGGAAGCGGCGCTGCGTGCTGGTGCTGGTTTAGTCCGAGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAG  >  NC_000913/4394807‑4395097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑