| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,330,402 | 0 | A | . | 24.2% | 83.5 / 24.3 | 34 | intergenic (+21/+15) | sohB/yciN | inner membrane protein, S49 peptidase family protein/DUF2498 protein YciN |
| Reads supporting (aligned to +/- strand): ref base A (11/14); new base . (4/4); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGC > NC_000913/1330254‑1330547 | gTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGa > 5:15947/1‑149 (MQ=255) cAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGttt < 7:100691/149‑1 (MQ=255) aaTGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGttt < 2:22521/148‑1 (MQ=255) gCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTaa < 1:64214/149‑1 (MQ=255) gaaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGata > 4:19061/2‑149 (MQ=255) aaaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGtttgtc < 8:88243/130‑4 (MQ=255) aaaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGtttgtc > 7:88243/1‑127 (MQ=255) cTCATTGACCGATTCTCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTc < 6:15947/149‑1 (MQ=255) cTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTc < 8:62797/149‑1 (MQ=255) aTTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt > 5:82153/1‑147 (MQ=255) aTTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt < 6:82153/147‑1 (MQ=255) aCCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAg > 7:55828/1‑148 (MQ=255) aCCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAg < 8:55828/148‑1 (MQ=255) ccGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGtttg > 5:28081/1‑105 (MQ=39) ccGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGtttg < 6:28081/106‑2 (MQ=39) gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAgat < 5:57421/103‑1 (MQ=255) gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAgat > 6:57421/1‑103 (MQ=255) tggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTgg < 3:19061/149‑1 (MQ=255) aGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAAt < 8:25150/149‑1 (MQ=255) aaaaGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAAc > 1:57945/1‑61 (MQ=255) aaaaGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAAc < 2:57945/61‑1 (MQ=255) cATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaa > 8:9770/1‑148 (MQ=255) tAAAAGACAAACGCGAGG‑CTAAGCCCTCGCGTTTTGATTTAATCAACCAGATTATATTTTTCTGAAAGAACATGGGCCAGGTGTTTTAACATATTAAACACAGAGTTGATTTTGTCTGTTGGAAATACTTGATAATCTAAAAagtaga > 7:32524/1‑147 (MQ=255) tAAAAGACAAACGCGAGG‑CTAAGACCTAGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagtagt < 8:32524/148‑1 (MQ=255) aaaaGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg > 1:84160/1‑149 (MQ=255) agcaaaaCGCGAGGTCTTAG‑CCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTg < 6:72052/106‑1 (MQ=38) agcaaaaCGCGAGGTCTTAG‑CCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTg > 5:72052/5‑110 (MQ=38) aGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCg > 6:54991/1‑149 (MQ=255) gACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCgg < 7:9770/149‑1 (MQ=255) aaaaCGCGAGGTCTTAG‑CCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGt > 3:53257/2‑148 (MQ=255) aaaCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa > 6:125594/1‑135 (MQ=255) aaaCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa < 5:125594/135‑1 (MQ=255) aaaCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa > 5:48763/1‑148 (MQ=255) gg‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCa < 2:84160/147‑1 (MQ=255) aaGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCGAGGTGTTTGAACATATAAAACACCGAGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCTACGGAAAATACCAGCACGc > 7:79272/1‑149 (MQ=255) | GTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGC > NC_000913/1330254‑1330547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |