| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,392,506 | 0 | C | G | 29.4% | 17.4 / 11.7 | 18 | noncoding (35/76 nt) | glyX | tRNA‑Gly |
| Reads supporting (aligned to +/- strand): ref base C (6/6); new base G (3/2); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAA‑GCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCT > NC_000913/4392389‑4392644 | ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAA‑GCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtgc < 8:91042/129‑4 (MQ=255)ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAA‑GCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtgc > 7:91042/1‑126 (MQ=255)ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAA‑GCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAg > 1:55085/1‑122 (MQ=40)ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAA‑GCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAg < 2:55085/122‑1 (MQ=40)ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAA‑GCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg > 2:177525/1‑127 (MQ=40) tCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAATTGATTC‑AAGCACAAGCCAACCAACACCGTAGTAGATCGAATGGTAGAGCACGAACTTGACAAGGTCGGGGTCGCGAGATCGAGTAACGATTCCAGCTCCAAAATTTg > 6:19357/1‑149 (MQ=9) tcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAA‑GCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg > 2:102260/1‑94 (MQ=255) tcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAA‑GCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg < 1:102260/94‑1 (MQ=255) ttccccGCCCCAAAATTTGAAAAGTTCTGCCAAGCAGAGACCACCCAA‑GCGTGAATAGGGCCGGTGGTCGACCACGACGTTGCCAAGGGCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGcaca < 5:19357/146‑1 (MQ=18) aaaGCACAGACCACCCAA‑GCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGt > 5:70909/1‑72 (MQ=34) aaaGCACAGACCACCCAA‑GCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGt < 6:70909/72‑1 (MQ=34) ttGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGc < 2:73764/121‑1 (MQ=40) ttGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGc > 1:73764/1‑121 (MQ=40) gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAACTTTGAAAGTGCTGTAAGGCACAGACCCCCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgc < 3:14755/138‑1 (MQ=255) gCACGACCTTGCCAAGGTCGGGGTCGCGAGATCGAGTCACGTTTCACGATCCAAAATTTGAAAGTGCTGTAAGGCAAATCCCACCCAAGCGGGAAAATATAAGAAGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGATCggagg > 4:14755/1‑145 (MQ=11) cccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTg > 1:86047/3‑105 (MQ=255) cccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTg < 2:86047/103‑1 (MQ=255) cccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct > 2:136547/3‑149 (MQ=255) | CCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAA‑GCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCT > NC_000913/4392389‑4392644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |