Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,374,672 G→A 5.2% T111M (ACG→ATG)  menE ← O‑succinylbenzoate‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,374,6720GA 5.2% 252.3 / 2.4 76T111M (ACG→ATG) menEO‑succinylbenzoate‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (36/36);  new base A (2/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01

AGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCG  >  NC_000913/2374525‑2374816
                                                                                                                                                   |                                                                                                                                                
aGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCTTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgt                                                                                                                                                 <  8:243033/149‑1 (MQ=255)
aGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgt                                                                                                                                                 >  8:10732/1‑149 (MQ=255)
aGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgt                                                                                                                                                 <  8:336309/149‑1 (MQ=255)
    gggCCTGATAAGTATGGACTGCGGCTTTCGGCAAACCGGTAGAACGCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGtt                                                                                                                                              <  8:309660/148‑1 (MQ=255)
      gCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCCGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTc                                                                                                                                           >  6:189100/1‑149 (MQ=255)
      gCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTc                                                                                                                                           <  7:56240/149‑1 (MQ=255)
         tGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTccc                                                                                                                                         >  6:148536/1‑148 (MQ=255)
               gTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGAGACGTTCAAGCCGGACACGTGTTTTCCCCATCCgg                                                                                                                                  >  4:11687/1‑149 (MQ=255)
                 aTGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCa                                                                                                                                >  1:329444/1‑149 (MQ=255)
                  tGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTcc                                                                                                                                          >  1:316067/1‑138 (MQ=255)
                  tGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTcc                                                                                                                                          <  2:316067/138‑1 (MQ=255)
                  tGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCAc                                                                                                                               >  5:194381/1‑149 (MQ=255)
                    gACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCAc                                                                                                                               >  5:325559/1‑147 (MQ=255)
                     aCAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCCTCCGGCACCag                                                                                                                            >  2:273364/1‑149 (MQ=255)
                        gCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGc                                                                                                                         <  6:194381/149‑1 (MQ=255)
                        gCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTCTCCCCATCCGGCACCa                                                                                                                             >  8:120198/1‑145 (MQ=255)
                        gCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTCTCCCCATCCGGCACCa                                                                                                                             <  7:120198/145‑1 (MQ=255)
                            cTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAAt                                                                                                                     >  4:186340/1‑149 (MQ=255)
                             tttCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAAtt                                                                                                                    >  4:264512/1‑149 (MQ=255)
                                  gCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGc                                                                                                               <  2:329444/149‑1 (MQ=255)
                                      aCCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAACCGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGt                                                                                                             >  4:354802/1‑147 (MQ=255)
                                        cGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGa                                                                                                         <  3:184267/149‑1 (MQ=255)
                                         ggTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGAt                                                                                                        <  3:328659/149‑1 (MQ=255)
                                              aaCCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTggg                                                                                                    <  3:188883/148‑1 (MQ=255)
                                              aaCCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTggg                                                                                                    >  4:188883/1‑148 (MQ=255)
                                                cccGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGgcag                                                                                                 >  7:152573/1‑149 (MQ=255)
                                                       tCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGTCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAAttct                                                                                          <  6:232262/149‑1 (MQ=255)
                                                           cGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAAttcttc                                                                                        >  8:103183/1‑147 (MQ=255)
                                                            gTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAg                                                                                     <  4:108726/149‑1 (MQ=255)
                                                                ttGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAAc                                                                                 >  7:342556/1‑149 (MQ=255)
                                                                    gCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACATGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAAttcttc                                                                                        <  1:48680/138‑1 (MQ=255)
                                                                    gCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACATGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAAttcttc                                                                                        >  2:48680/1‑138 (MQ=255)
                                                                    gCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACATGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAAttcttc                                                                                        <  1:42382/138‑1 (MQ=255)
                                                                    gCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACATGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAAttcttc                                                                                        >  2:42382/1‑138 (MQ=255)
                                                                         gACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGc                                                                        <  2:213540/149‑1 (MQ=255)
                                                                                 ggCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAgg                                                                <  7:10732/149‑1 (MQ=255)
                                                                                  gCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAggg                                                               <  7:354458/149‑1 (MQ=255)
                                                                                   cTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGt                                                              <  3:186340/149‑1 (MQ=255)
                                                                                    tGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGtt                                                             >  8:313178/1‑149 (MQ=255)
                                                                                    tGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGt                                                              >  3:230195/1‑148 (MQ=255)
                                                                                     gCCAAGTAGCGGCATGAGCGCCTTCAACCAGCAGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATACGGCACCAGAGCAAATTGCAGCGTCAGATTAGGCACCAATTCTTAAAGCAACGTTTGCGGACTCTGAGGGtt                                                             >  6:79948/1‑148 (MQ=255)
                                                                                       cACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCAc                                                          <  7:20257/149‑1 (MQ=255)
                                                                                         cGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACgg                                                        <  1:228080/149‑1 (MQ=255)
                                                                                         cGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGGTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACgg                                                        <  4:304796/149‑1 (MQ=255)
                                                                                          gTAGCGGCATGTGCGCCTTCAACCAGCTGAATGGGCAGCGACGTTAATGCCGGAAACGTGGTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACggg                                                       <  1:273364/149‑1 (MQ=255)
                                                                                                gCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAg                                                                                     <  2:235399/113‑1 (MQ=255)
                                                                                                gCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAg                                                                                     >  1:235399/1‑113 (MQ=255)
                                                                                                  aTGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGAAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACAccc                                               >  1:111401/1‑149 (MQ=255)
                                                                                                   tgtgCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAggg                                                               <  7:324672/132‑1 (MQ=255)
                                                                                                   tgtgCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAggg                                                               >  8:324672/1‑132 (MQ=255)
                                                                                                        gcCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAggg                                                               <  8:120217/127‑1 (MQ=255)
                                                                                                        gcCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAggg                                                               >  7:120217/1‑127 (MQ=255)
                                                                                                          cTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCAc                                                          <  6:37753/130‑1 (MQ=255)
                                                                                                          cTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCAc                                                          >  5:37753/1‑130 (MQ=255)
                                                                                                          cTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCg                                       >  8:81214/1‑149 (MQ=255)
                                                                                                               aacAGCTTAATCAGCAGAGACGATAAAGACGGAAACGTGTTTTACCCAACCGCCACCAGAGCAAATTGCAGCGTCAGATACGGCAGCAATTCTAAAAGCAACGGTTGCGGCACCTGAGGGTTCACGGGCAACACACCCTCCCCGCAtt                                   >  4:169805/3‑148 (MQ=255)
                                                                                                                ccAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGc                                 <  6:325559/149‑1 (MQ=255)
                                                                                                                 cAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCAt                                    <  6:389745/145‑1 (MQ=255)
                                                                                                                 cAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCAt                                    >  5:389745/1‑145 (MQ=255)
                                                                                                                     tGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTaa                            <  2:111401/149‑1 (MQ=255)
                                                                                                                      gAATGTGCAGCGACGTTAATGCCTGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAAc                                                                                 <  8:95244/95‑1 (MQ=255)
                                                                                                                      gAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAAc                                                                                 >  7:89298/1‑95 (MQ=255)
                                                                                                                      gAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAAc                                                                                 >  7:95244/1‑95 (MQ=255)
                                                                                                                      gAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAAc                           <  8:138822/149‑1 (MQ=255)
                                                                                                                       aaTGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAAc                           <  3:264512/148‑1 (MQ=255)
                                                                                                                            gCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACgccagc                     >  8:75643/1‑149 (MQ=255)
                                                                                                                            gCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACgccagc                     >  5:190453/1‑149 (MQ=255)
                                                                                                                                  aCGTTAATGCCGGAAACGTGTTTTCCCCATCCGGAACCAGAGCAAATTGGAGCGTCAGATTGGGAATCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCAGCATTGCACTAACgccagcacggc                >  4:293888/1‑143 (MQ=255)
                                                                                                                                      tAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACggg                                                       >  4:355718/1‑105 (MQ=255)
                                                                                                                                      tAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACggg                                                       <  3:355718/105‑1 (MQ=255)
                                                                                                                                        aTGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGagcagc         <  7:103183/149‑1 (MQ=255)
                                                                                                                                           ccGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGtt      <  3:11687/149‑1 (MQ=255)
                                                                                                                                             ggAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGagca           >  6:404026/1‑142 (MQ=255)
                                                                                                                                             ggAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGagca           <  5:404026/142‑1 (MQ=255)
                                                                                                                                             ggAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTCGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTg    >  1:249748/1‑149 (MQ=255)
                                                                                                                                              gAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTgc   <  5:148536/149‑1 (MQ=255)
                                                                                                                                               aaaCGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCTGATTGGGCAGCAATTCTGCAAGCATCGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTgcg  <  3:293888/149‑1 (MQ=255)
                                                                                                                                               aaaCGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTgcg  <  3:169805/149‑1 (MQ=255)
                                                                                                                                               aaaCGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTgcg  >  1:238138/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCG  >  NC_000913/2374525‑2374816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: