Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 53,272 T→G 5.9% M49L (ATG→CTG)  pdxA ← 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091353,2720TG 5.9% 240.8 / 3.6 78M49L (ATG→CTG) pdxA4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base T (35/38);  new base G (2/3);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACATAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAG  >  NC_000913/53126‑53419
                                                                                                                                                  |                                                                                                                                                   
cacaTAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATc                                                                                                                                                    <  3:254593/148‑1 (MQ=255)
 acaTCATGCCCATTTTCAACCGCTAACTGCCACGCAGTGACATGTGCACGTAGCGCGACATGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGTAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCg                                                                                                                                                   <  4:377533/148‑1 (MQ=255)
 acaTAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCg                                                                                                                                                   >  1:356935/1‑148 (MQ=255)
  caTAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGc                                                                                                                                                  >  6:47800/1‑148 (MQ=255)
    tAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTg                                                                                                                                                >  3:239423/1‑148 (MQ=255)
      aTGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGcc                                                                                                                                              <  6:18413/148‑1 (MQ=255)
         cccATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCgg                                                                                                                                           >  8:62548/1‑148 (MQ=255)
          ccATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGt                                                                                                                                          <  5:47800/148‑1 (MQ=255)
          ccATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGt                                                                                                                                          <  3:378709/148‑1 (MQ=255)
             ttttCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGGGCAGCGATGCTCGGtttg                                                                                                                                       <  5:208584/148‑3 (MQ=255)
             ttttCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTgg                                                                                                                                       <  2:122438/148‑1 (MQ=255)
              tttCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTgg                                                                                                                                       >  2:291899/1‑147 (MQ=255)
               ttCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGGAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGt                                                                                                                                      <  2:356935/147‑1 (MQ=255)
                    ccGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGgag                                                                                                                                <  1:291899/148‑1 (MQ=255)
                      gCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGTCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGagag                                                                                                                              <  6:423802/148‑1 (MQ=255)
                       cTAACTGCCCGGCAGTGACAGGTTGACGTAGCTCGACAGGAAGTATGGTTTATGTGCCGGCTGTTTGCGGTTGTGCAGGGGATTTGGGGGAATAAGGGGGGAGGGTGAGCGGCAAACCGTGCATCGCTGccgg                                                                                                                                            <  4:407350/133‑3 (MQ=255)
                       cTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGt                                                                                                                             <  4:239423/148‑1 (MQ=255)
                       cTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGt                                                                                                                             <  6:164210/148‑1 (MQ=255)
                              ccccGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCAt                                                                                                                        <  4:132541/146‑1 (MQ=255)
                               cccGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGTGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCgg                                                                                                                     <  4:19926/148‑1 (MQ=255)
                                         cAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAaacaac                                                                                                           <  2:54514/148‑1 (MQ=255)
                                         cAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAaacaac                                                                                                           >  6:360409/1‑148 (MQ=255)
                                           ggTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTTCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTg                                                                                                                            >  7:54194/1‑129 (MQ=255)
                                             tGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGgag                                                                                                                                >  3:134953/1‑123 (MQ=255)
                                             tGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGgag                                                                                                                                <  4:134953/123‑1 (MQ=255)
                                             tGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGt                                                                                                       <  2:376709/148‑1 (MQ=255)
                                                 cGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGgaga                                                                                                                               >  7:452230/1‑120 (MQ=255)
                                                 cGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGgaga                                                                                                                               <  8:452230/120‑1 (MQ=255)
                                                  gTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGAc                                                                                                  <  8:83125/148‑1 (MQ=255)
                                                   tAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGgag                                                                                                                                >  1:238180/1‑117 (MQ=255)
                                                   tAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGgag                                                                                                                                <  2:238180/117‑1 (MQ=255)
                                                   tAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGAcc                                                                                                 >  5:46131/1‑148 (MQ=255)
                                                        cgACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCa                                                                                            >  8:345369/1‑148 (MQ=255)
                                                        cgACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCa                                                                                            >  2:40986/1‑148 (MQ=255)
                                                          aCAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACt                                                                                          >  3:123379/1‑148 (MQ=255)
                                                           cAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCAGCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTc                                                                                         <  7:426241/148‑1 (MQ=255)
                                                               aaGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCgg                                                                                                                                           >  3:7075/1‑94 (MQ=255)
                                                               aaGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCgg                                                                                                                                           <  4:7075/94‑1 (MQ=255)
                                                               aaGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGc                                                                                     <  6:46131/148‑1 (MQ=255)
                                                                   aGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCtgtg                                                                                 >  4:156745/1‑148 (MQ=255)
                                                                       ttAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAg                                                                             >  8:405434/1‑148 (MQ=255)
                                                                                ccGCAGTTTGCGGTTTTGCAGGGGAGTAGGGCGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGCCACAAACAACCAGATCGACCGGCCACTCACGCTGTGCAAGCTGGACAAc                                                                    <  5:121792/148‑1 (MQ=255)
                                                                                ccGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGTTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACAAGTTCGACCGGCCACTAACGATGTGCAAGCTGCACAAc                                                                    >  6:121792/1‑148 (MQ=255)
                                                                                ccGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCTAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGTCCACTCCCGCTGTGAAAGCTGGACAAc                                                                    >  6:127668/1‑148 (MQ=255)
                                                                                ccGAAGTTTGCGGTTTTTCAGGGTAGTTGGGGTAATAAGGGCGGAGTGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAAc                                                                    <  5:127668/148‑1 (MQ=255)
                                                                                  gCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTa                                                                  <  5:360409/148‑1 (MQ=255)
                                                                                            gTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACACCTAAGTCCGGGc                                                         >  8:392420/1‑147 (MQ=255)
                                                                                             ttGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCtgtg                                                                                 <  7:143868/122‑1 (MQ=255)
                                                                                             ttGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCtgtg                                                                                 >  8:143868/1‑122 (MQ=255)
                                                                                              tgtgCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCg                                                                                                    >  6:187718/1‑102 (MQ=255)
                                                                                              tgtgCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCg                                                                                                    <  5:187718/102‑1 (MQ=255)
                                                                                                tgCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATc                                                    <  3:156745/148‑1 (MQ=255)
                                                                                                 gCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACaa                                                                     >  2:446952/1‑130 (MQ=255)
                                                                                                 gCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACaa                                                                     <  1:446952/130‑1 (MQ=255)
                                                                                                  cAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCa                                                                               <  8:2368/119‑1 (MQ=255)
                                                                                                  cAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCa                                                                               >  7:2368/1‑119 (MQ=255)
                                                                                                     gggAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTgg                                                                         >  8:96339/1‑122 (MQ=255)
                                                                                                     gggAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTgg                                                                         <  7:96339/122‑1 (MQ=255)
                                                                                                           tGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGc                                           <  3:131661/146‑1 (MQ=255)
                                                                                                           tGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGc                                           >  4:131661/1‑146 (MQ=255)
                                                                                                             ggggAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGc                                       >  4:351344/1‑148 (MQ=255)
                                                                                                                 aaTAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCggg                                   <  7:405434/148‑1 (MQ=255)
                                                                                                                   tAAGGGCGGAGGGTGAGCGGCTAACCGAGCATCGCTGGCCTGTTGGTAAGGAGAGTGGCATCGGCAGAAACAACCAGTTCGACCGGCCACTGAGGCTGTGCAAGCTGGACAACTATGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAg                                 <  2:339354/148‑1 (MQ=255)
                                                                                                                    aaGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGt                                >  4:174902/1‑148 (MQ=255)
                                                                                                                       ggCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCacaaa                                                                                                               >  2:36634/1‑66 (MQ=255)
                                                                                                                       ggCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCacaaa                                                                                                               <  1:36634/66‑1 (MQ=255)
                                                                                                                       ggCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGAt                             >  7:27947/1‑148 (MQ=255)
                                                                                                                       ggCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCACCGGGAGTGAt                             <  7:345369/148‑1 (MQ=255)
                                                                                                                        gCGGAGGGGGAGCGGCAAACCGAGCAGCGCTGCCCGGTTGGTAAGGAGAGGGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCGGGACAACTAAGGCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATc                            <  7:392420/148‑1 (MQ=255)
                                                                                                                            aGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCacaa                        <  6:422749/148‑1 (MQ=255)
                                                                                                                               gTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAacac                     >  7:436401/1‑148 (MQ=255)
                                                                                                                                 gAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGt                   >  3:299419/1‑148 (MQ=255)
                                                                                                                                     taccaaCCGGGCAGCGATGCTCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTggg               >  7:206706/5‑148 (MQ=255)
                                                                                                                                      gCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGt              >  1:106936/1‑148 (MQ=255)
                                                                                                                                          aCCGGGCAGCGATGCTCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTAGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTa          >  6:415929/1‑148 (MQ=255)
                                                                                                                                            cGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGAGCCCGCCGGGATTg                               <  6:228824/125‑1 (MQ=255)
                                                                                                                                            cGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGAGCCCGCCGGGATTg                               >  5:228824/1‑125 (MQ=255)
                                                                                                                                               gCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCAt     >  8:406334/1‑148 (MQ=255)
                                                                                                                                                  tCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAg  <  3:174902/148‑1 (MQ=255)
                                                                                                                                                  tCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAg  <  8:436401/148‑1 (MQ=255)
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CACATAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGTGATCACAACACGTTGGGTTTTAACCATTAG  >  NC_000913/53126‑53419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: