Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 151,715 G→A 5.3% R173C (CGC→TGC)  yadL ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913151,7150GA 5.3% 364.2 / 5.2 112R173C (CGC→TGC) yadLputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base G (47/59);  new base A (3/3);  total (50/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

TTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAG  >  NC_000913/151571‑151852
                                                                                                                                                |                                                                                                                                         
ttCATCTGGTTACGTTGAGTGGGTTGCATATATAGTTCCTGATTCTGTAAGTCTGTTAGTTATTGGTTACTGTTAATGTGGCTTGTGCAGAAAACTTACCCGGAGTGAGATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGt                                                                                                                                          <  5:4294/146‑1 (MQ=255)
  catcaGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGc                                                                                                                                        <  7:411363/146‑1 (MQ=255)
     caGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCa                                                                                                                                   >  3:266287/1‑148 (MQ=255)
      aGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTTGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCAt                                                                                                                                  <  1:361898/148‑1 (MQ=255)
        cTTACGTTGAGTGGGATGAATATATAGTTCCTGAATCTGAAAGTCTGTTAGTTATAGGTTACTGTATATGTGGCTGGTGAAGAAAACTTACCCGCAGTGACAAAGATGAAGGTATTATTTTTTTACACGCCCAGGCGTGCACTCATCg                                                                                                                                >  6:316488/1‑148 (MQ=255)
         ttACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCg                                                                                                                                >  2:391298/1‑147 (MQ=255)
          tACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCAt                                                                                                                                  <  3:2195/144‑1 (MQ=255)
            cGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGat                                                                                                                             >  4:252127/1‑147 (MQ=255)
              ttGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGataa                                                                                                                           >  4:113852/1‑147 (MQ=255)
                 aGTGGGATGCATATATAGTTCCTGAATCTGTTAGTCTGATAGTTATAGGTTACTGTAAATGTGGCCGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACGAGGCGTGCACTCTTCGGAtaataa                                                                                                                        <  2:398449/147‑1 (MQ=255)
                   tGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACg                                                                                                                     <  7:182655/148‑1 (MQ=255)
                     ggATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGa                                                                                                                    <  3:28494/147‑1 (MQ=255)
                     ggATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGAc                                                                                                                   <  3:252127/148‑1 (MQ=255)
                            tatataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGAc                                                                                                                   >  7:408205/1‑141 (MQ=255)
                            tatataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGAc                                                                                                                   <  8:408205/141‑1 (MQ=255)
                             atataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTTCTGTAAATGTGGATGGTGCAGAAAAGTTACCCGCAGTGACATTGTTTAAGGTTTTATTTTTTTCGACGACCAGGCATGCAGTCATAGGATTATAAAGGACCGCAGTgg                                                                                                           >  7:358305/1‑148 (MQ=255)
                             atataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGat                                                                                                                             >  5:343314/1‑130 (MQ=255)
                             atataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATaaa                                                                                                                       <  6:99119/136‑1 (MQ=255)
                             atataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATaaa                                                                                                                       >  5:99119/1‑136 (MQ=255)
                             atataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTgg                                                                                                           >  4:297974/1‑148 (MQ=255)
                             atataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTgg                                                                                                           >  6:97782/1‑148 (MQ=255)
                             atataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTg                                                                                                            >  1:10745/1‑147 (MQ=255)
                             atataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCAGGCACTCATCGGATAATAAACGACCGCATTgt                                                                                                           >  7:358304/1‑147 (MQ=255)
                             atataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACACGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACAAGGCATGCACTCATCGGATAATAAACGACCGCATTgg                                                                                                           >  7:352137/1‑148 (MQ=255)
                              tataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGc                                                                                                          >  3:137180/1‑148 (MQ=255)
                              tataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGc                                                                                                          >  3:181567/1‑148 (MQ=255)
                                 aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTg                                                                                                        >  7:104016/1‑147 (MQ=255)
                                  gTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTCAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGc                                                                                                       <  2:275312/147‑1 (MQ=255)
                                  gTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGc                                                                                                       <  3:297974/147‑1 (MQ=255)
                                  gTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGc                                                                                                       <  3:272832/147‑1 (MQ=255)
                                    tCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGt                                                                                                    >  2:343155/1‑148 (MQ=255)
                                      cTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGt                                                                                                    >  6:16905/1‑146 (MQ=255)
                                        gAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTg                                                                                                   >  3:162855/1‑145 (MQ=255)
                                         aaTCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGgag                                                                                                <  1:343155/147‑1 (MQ=255)
                                         aaTCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGgag                                                                                                <  4:266287/147‑1 (MQ=255)
                                          aTCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGAt                                                                                              <  1:188223/148‑1 (MQ=255)
                                            cTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTATACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTg                                                                                            >  2:256133/1‑148 (MQ=255)
                                               tAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTg                                                                                            <  1:391298/145‑1 (MQ=255)
                                               tAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGtt                                                                                          <  3:113852/147‑1 (MQ=255)
                                               tAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGtt                                                                                          <  5:97782/147‑1 (MQ=255)
                                               tAAGTCGGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTg                                                                                            <  8:320073/145‑1 (MQ=255)
                                                aaGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGataa                                                                                                                           >  4:117028/1‑113 (MQ=255)
                                                   tCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGAtaataa                                                                                                                        <  5:16854/113‑1 (MQ=255)
                                                   tCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGAtaataa                                                                                                                        >  6:16854/1‑113 (MQ=255)
                                                   tCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGgag                                                                                                <  1:111629/137‑1 (MQ=255)
                                                   tCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGgag                                                                                                >  2:111629/1‑137 (MQ=255)
                                                     tGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCtt                                                                                   <  8:438464/148‑1 (MQ=255)
                                                      gttagttaTAGGTTACTGTAAATGTTTCTGGAGCAGAAAACTTACCCGCAGTGACATTGATTACGGTTTTATTTTTTACCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTCCCTGCAGAGGAGATTGTTACCTCTtg                                                                                  >  8:82392/1‑148 (MQ=255)
                                                      gttagttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCa                                                                                                      >  4:190565/1‑128 (MQ=255)
                                                      gttagttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCa                                                                                                      <  3:190565/128‑1 (MQ=255)
                                                       ttagttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTtgt                                                                                 <  2:439207/148‑1 (MQ=255)
                                                        tagttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTAGTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGGGGAGATTGTTACCTCTtgtg                                                                                >  6:281162/1‑148 (MQ=255)
                                                         agttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTtgtg                                                                                <  4:162855/147‑1 (MQ=255)
                                                         agttaTAGGTTACTGTAAATGTGGCTGGGGCATAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGCCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTtgtg                                                                                <  1:293441/147‑1 (MQ=255)
                                                            tataGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGataata                                                                                                                         <  1:139614/103‑1 (MQ=255)
                                                            tataGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGataata                                                                                                                         >  2:139614/1‑103 (MQ=255)
                                                            tataGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCTCATTGGCTGCAGTGGAGATTg                                                                                            >  8:317502/1‑132 (MQ=255)
                                                            tataGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTg                                                                                            <  7:317502/132‑1 (MQ=255)
                                                                gggtagtGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTTCGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTgg                                                                         <  1:50485/141‑1 (MQ=255)
                                                                ggTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGGGGAGATTGTTACCTCTTGTGATGCTgg                                                                         <  1:141454/147‑1 (MQ=255)
                                                                  ttACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGgtgt                                                                      >  3:259885/1‑148 (MQ=255)
                                                                  ttACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGgtgt                                                                      <  8:240603/148‑1 (MQ=255)
                                                                   tACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTtgtg                                                                                >  2:350798/1‑137 (MQ=255)
                                                                   tACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTtgtg                                                                                <  1:350798/137‑1 (MQ=255)
                                                                       gTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGAtaataa                                                                                                                        >  7:433143/1‑93 (MQ=255)
                                                                       gTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGAtaataa                                                                                                                        <  8:433143/93‑1 (MQ=255)
                                                                        tAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTacac                                                                 <  2:322764/147‑1 (MQ=255)
                                                                        tAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTaca                                                                  <  1:311477/146‑1 (MQ=255)
                                                                         aaaTGTGGCTGGTGAAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACctct                                                                                    <  8:62031/127‑1 (MQ=255)
                                                                            tgtgGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTa                                                            <  1:386546/148‑1 (MQ=255)
                                                                              tgGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTaaa                                                          <  8:104016/148‑1 (MQ=255)
                                                                                 cTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATtg                                                       >  3:238112/1‑148 (MQ=255)
                                                                                    gTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATtgtg                                                     <  4:137180/147‑1 (MQ=255)
                                                                                    gTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTgtgt                                                    <  4:238112/148‑1 (MQ=255)
                                                                                    gTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGAGAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTgtgt                                                    >  2:176987/1‑148 (MQ=255)
                                                                                    gTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTAATATTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTgtgt                                                    <  8:431134/148‑1 (MQ=255)
                                                                                    gTGCAGAAAACTTACCCGCAGTGACAATGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGATACCTCTTGTGATGCTGGTGTTACACAGCTAAATtgtg                                                     <  5:316488/147‑1 (MQ=255)
                                                                                        aGAAAACTTACCCGCAGTTACTTTGTTTACGGATTTATTTTTTTCCACGACCAGGCATGCAGTCATGGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTAACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCa                                                <  8:352137/148‑1 (MQ=255)
                                                                                        aGAAAAATTACCCTCTGTGACATTTATTACCCTTTTATTTTATTCCACGAGCAGGCATGCACTCATCGGATAATAAACGACCTCATTGCCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCa                                                <  8:358305/148‑1 (MQ=255)
                                                                                                tACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACctct                                                                                    <  6:96502/104‑1 (MQ=255)
                                                                                                tACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACctct                                                                                    >  5:96502/1‑104 (MQ=255)
                                                                                                  ccggctgTGAGATTGTTTACGTTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGTATAATAAACGAGCGCTTTGGCTGCAGTTGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTg                                      <  7:82392/142‑1 (MQ=255)
                                                                                                    cGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCa                                                                                                               >  4:5273/1‑73 (MQ=255)
                                                                                                    cGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCa                                                                                                               <  3:5273/73‑1 (MQ=255)
                                                                                                    cGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGcc                                    >  2:88789/1‑148 (MQ=255)
                                                                                                           aCATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGtt                              <  4:181567/147‑1 (MQ=255)
                                                                                                              ttGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAg                          >  3:159838/1‑148 (MQ=255)
                                                                                                               tGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGt                         >  1:448299/1‑148 (MQ=255)
                                                                                                                        ttttATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGAc                <  1:256133/148‑1 (MQ=255)
                                                                                                                         tttATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTAc                            >  6:425544/1‑135 (MQ=255)
                                                                                                                         tttATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTAc                            <  5:425544/135‑1 (MQ=255)
                                                                                                                           tATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCGAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACtt              >  8:108309/1‑147 (MQ=255)
                                                                                                                               tttttCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTc                     >  5:368362/1‑136 (MQ=255)
                                                                                                                               tttttCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTc                     <  6:368362/136‑1 (MQ=255)
                                                                                                                                     cccGACCAGGCGTGCACTCATCGGATAATAACCGACCGCATTGGCTGCAGGGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACCGCTAAATTGTGTTGCCCGCCCTGTTGCCGGAGTTACAGTGCTCCCGACTTCACCGGCGa     >  5:367827/3‑146 (MQ=255)
                                                                                                                                      aCGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAg  >  2:1197/1‑148 (MQ=255)
                                                                                                                                      aCGACCAGGCGTGCACTCATCGGATAATAAAAGAACGCATTGGCTGCAGTGGAGATTGTTACCACTTGTGATGCTGGTGTTACACAGCTAAATTATGTTGCACTCCCTGTTGGCGGATTTACAGTGCTCCAGACTTCAAAGGCGACAg  >  5:419735/1‑148 (MQ=255)
                                                                                                                                             ggCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTgtgt                                                    >  4:357408/1‑91 (MQ=255)
                                                                                                                                             ggCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTgtgt                                                    <  3:357408/91‑1 (MQ=255)
                                                                                                                                                gTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCgg                                  >  4:385157/1‑106 (MQ=255)
                                                                                                                                                |                                                                                                                                         
TTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAG  >  NC_000913/151571‑151852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: