Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,478,995 A→G 5.4% K257R (AAA→AGA)  ynbB → putative CDP‑diglyceride synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,478,9950AG 5.4% 230.4 / 2.1 74K257R (AAA→AGA) ynbBputative CDP‑diglyceride synthase
Reads supporting (aligned to +/- strand):  ref base A (39/31);  new base G (2/2);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTTTTTATTTTATACGCTACTGCTGTTACTGAAGGAAAATAGAGAAAAT  >  NC_000913/1478856‑1479139
                                                                                                                                           |                                                                                                                                                
ggcggcGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGat                                                                                                                                           <  1:272152/147‑1 (MQ=255)
    gcgTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTgg                                                                                                                                      <  7:152432/148‑1 (MQ=255)
        catcaCCATCATGATTGCGTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGgtgt                                                                                                                                   >  7:223251/1‑147 (MQ=255)
        catcaCCATCATGATTGCGTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGgtgt                                                                                                                                   >  7:52942/1‑147 (MQ=255)
        catcaCCATCATGATTGCGTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGgtgt                                                                                                                                   >  7:59970/1‑147 (MQ=255)
          tcaCCATCATGACTGCGTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGGGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTa                                                                                                                                 >  7:421427/1‑147 (MQ=255)
           caCCATCATGATTGCGTCACTGATTATCGGACCGTTACTGACACCGCTATATACATTACAGGCTTTATTAGCGGGTTTGTTAATTGGTATTAGTGGGTTTTGCGGCGATGTCGGGATGTCAGCCATCAAACGAGATATTGGGGTTaa                                                                                                                                <  7:28972/147‑1 (MQ=255)
            aCCATCATGATTGCGTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTATTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAACCGAGATATTGGTGTTAAAg                                                                                                                              >  8:28972/1‑148 (MQ=255)
            aCCATCATGATTGCGTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTgg                                                                                                                                      <  1:16378/140‑1 (MQ=255)
            aCCATCATGATTGCGTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTgg                                                                                                                                      >  2:16378/1‑140 (MQ=255)
               atcatGATTGCGTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGa                                                                                                                             >  3:54782/1‑146 (MQ=255)
               atcatGATTGCGTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGa                                                                                                                             >  3:60574/1‑146 (MQ=255)
                 catGATTGCGTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAGACGAGATATTGGTGTTAAAGATAGc                                                                                                                         >  2:90729/1‑148 (MQ=255)
                 catGATTGCGTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGc                                                                                                                         >  8:336924/1‑148 (MQ=255)
                          gTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTa                                                                                                                <  4:60574/148‑1 (MQ=255)
                          gTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTa                                                                                                                <  8:59970/148‑1 (MQ=255)
                          gTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTa                                                                                                                <  4:54782/148‑1 (MQ=255)
                          gTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTa                                                                                                                <  8:52942/148‑1 (MQ=255)
                           tCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTAt                                                                                                               >  7:290608/1‑148 (MQ=255)
                            cACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTgg                                                                                                                                      >  6:216812/1‑124 (MQ=255)
                            cACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTgg                                                                                                                                      <  5:216812/124‑1 (MQ=255)
                                 aTTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGGGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGcc                                                                                                           <  8:421427/146‑1 (MQ=255)
                                    aTCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGa                                                                                                       <  8:290608/147‑1 (MQ=255)
                                           cGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAGACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCgg                                                                                               <  1:90729/148‑1 (MQ=255)
                                                   acacCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAGACGAGATATTGTTGTTAAAGATAGAGGAAAACTATTGCCAGGACATGGCGGACTCCtt                                                                                        >  5:42411/1‑147 (MQ=255)
                                                     acCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGAc                                                                                     <  8:223251/148‑1 (MQ=255)
                                                       cGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAg                                                                                   >  2:86853/1‑148 (MQ=255)
                                                        gCTAAATAAATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTCCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGATAAAGATAGCGGAAAACTATTGCCAGGACATGTCGGACTCCTTCACAg                                                                                   >  6:79112/1‑147 (MQ=255)
                                                           aaaTACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGAtt                                                                               >  8:260257/1‑148 (MQ=255)
                                                           aaaTACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGAtt                                                                               >  2:399433/1‑148 (MQ=255)
                                                            aaTACATTACTGGCATTATTAGGGGTTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAGACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTg                                                                              <  6:42411/148‑1 (MQ=255)
                                                            aaTACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAACCTATTGCCAGGACATGGCGGACTcc                                                                                          <  4:226766/136‑1 (MQ=255)
                                                            aaTACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAACCTATTGCCAGGACATGGCGGACTcc                                                                                          >  3:226766/1‑136 (MQ=255)
                                                                cATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCgg                                                                                               >  6:78089/1‑127 (MQ=255)
                                                                cATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCgg                                                                                               <  5:78089/127‑1 (MQ=255)
                                                                cATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACt                                                                           >  6:138237/1‑147 (MQ=255)
                                                                   tACAGGCATTATTAGCGGGTTTGTAAATTGCTATTAGTGGTTTATGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGTCATGGCGGACTCCTTGACAGGATTGACTCAt                                                                        >  4:6617/1‑147 (MQ=255)
                                                                     cAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTaa                                                                     >  3:254399/1‑148 (MQ=255)
                                                                         cATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTg                                                                              <  8:261297/135‑1 (MQ=255)
                                                                         cATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTg                                                                              >  7:261297/1‑135 (MQ=255)
                                                                         cATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAAtttt                                                                 <  5:138237/148‑1 (MQ=255)
                                                                          attattAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTc                                                                <  1:86853/148‑1 (MQ=255)
                                                                           ttattaGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCa                                                               >  1:397593/1‑148 (MQ=255)
                                                                            tattaGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGaaa                                                                                                                    >  2:172400/1‑94 (MQ=255)
                                                                            tattaGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGaaa                                                                                                                    <  1:172400/94‑1 (MQ=255)
                                                                            tattaGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCAc                                                              >  6:368962/1‑148 (MQ=255)
                                                                            tattaGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCAc                                                              >  4:30826/1‑148 (MQ=255)
                                                                               taGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATg                                                                                                   >  1:343967/1‑108 (MQ=255)
                                                                               taGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATg                                                                                                   <  2:343967/108‑1 (MQ=255)
                                                                                       ttGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGcc                                                                                                           >  1:119289/1‑92 (MQ=255)
                                                                                       ttGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGcc                                                                                                           <  2:119289/92‑1 (MQ=255)
                                                                                             aTTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTa                                                                      <  8:122385/123‑1 (MQ=255)
                                                                                             aTTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTa                                                                      >  7:122385/1‑123 (MQ=255)
                                                                                              ttGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATA‑‑GGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTttttatt                                          >  5:124085/1‑148 (MQ=255)
                                                                                               tGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTttttat                                           >  8:93758/1‑148 (MQ=255)
                                                                                                ggTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTttttat                                           <  3:30826/147‑1 (MQ=255)
                                                                                                    ttAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGTAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCTTTAATTTTCACCGCTCCGGTATTTTtttattttat                                      <  4:37331/148‑1 (MQ=255)
                                                                                                    ttAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTttttatttta                                       >  3:96638/1‑147 (MQ=255)
                                                                                                    ttAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTTtttattttat                                      <  5:368962/148‑1 (MQ=255)
                                                                                                     tAGTGGTTTTTGCGGCGATGTCCTGATGTCAGCCATCAAACGAGATAATGTTGTTAAAGATAGCGGAAAACTATTGCATTGACATGGCGGAATCATTGACAGGATTGAATCATTAATTTTCACCGCTCCGGTATTTttatatattaa                                      >  4:209847/1‑146 (MQ=255)
                                                                                                           tttttGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTTTTTATTTTATACGCTAc                               <  7:127288/148‑1 (MQ=255)
                                                                                                                  ggcgATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTTTTTATTTTATACGCTACTGCTGtt                        <  4:254399/148‑1 (MQ=255)
                                                                                                                      aTGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCACCGGTATTTTTTTATTTTATACGCTACTGCTGTTACt                     >  7:431180/1‑147 (MQ=255)
                                                                                                                       tGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGAc                                                                                     >  6:220407/1‑82 (MQ=255)
                                                                                                                       tGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGAc                                                                                     <  5:220407/82‑1 (MQ=255)
                                                                                                                              atggcttccATGAAACGATATATTCGTGTTAAAGATAGATCAAAACTATTGCCAGGAGATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTGACCGCTCCGGTATTTTTTTATTTTATACGCTACTGCTGTTACTGAAGGaaa             <  3:6617/140‑1 (MQ=255)
                                                                                                                              aTGTCAGGCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCGAGGACATGTCTGACTCATTGACATTATTGACTCATTAATTTTCACCGCTCCGGTATTTTTTTATTTTATACGCTACTGCTGTTACTGAAGGaaaa            <  5:79112/148‑1 (MQ=255)
                                                                                                                              aTGTCAGCCATCAAACGAGATATTGGTGTTAAAGATA‑‑GGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTTTTTATTTTATACGCTACTGCTGTTACTGAAGGAAAAt           <  6:124085/147‑1 (MQ=255)
                                                                                                                                 tCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTTTTTATTTTATACGCTACTGCTGTTACTGAAGGaaaa            >  3:330223/1‑145 (MQ=255)
                                                                                                                                  cAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTTTTTATTTTATACGCTACTGCTGTTACTGAAGGAAAATa          >  8:180247/1‑146 (MQ=255)
                                                                                                                                    gCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTTTTTATTTTATACGCTACTGCTGTTACTGAAGGAAAATagag       >  8:332480/1‑147 (MQ=255)
                                                                                                                                        tCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTTTTTATTTTATACGCt                                 >  2:429490/1‑117 (MQ=255)
                                                                                                                                        tCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTTTTTATTTTATACGCt                                 <  1:429490/117‑1 (MQ=255)
                                                                                                                                        tCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTTTTTATTTTATACGCTACTGCTGTTACTGAAGGAAAATAGAGAAAAt  >  8:120266/1‑148 (MQ=255)
                                                                                                                                           aaCGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTTTTTATTTTATACGCt                                 <  5:35655/114‑1 (MQ=255)
                                                                                                                                           aaCGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTTTTTATTTTATACGCt                                 >  6:35655/1‑114 (MQ=255)
                                                                                                                                           |                                                                                                                                                
GGCGGCGTCATCACCATCATGATTGCGTCACTGATTATCGGACCGTTACTGACACCGCTAAATACATTACAGGCATTATTAGCGGGTTTGTTAATTGGTATTAGTGGTTTTTGCGGCGATGTCGTGATGTCAGCCATCAAACGAGATATTGGTGTTAAAGATAGCGGAAAACTATTGCCAGGACATGGCGGACTCCTTGACAGGATTGACTCATTAATTTTCACCGCTCCGGTATTTTTTTATTTTATACGCTACTGCTGTTACTGAAGGAAAATAGAGAAAAT  >  NC_000913/1478856‑1479139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: