Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,714,557	A→T	6.2%	T61S (ACT→TCT)	gstA →	glutathionine S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,714,557	0	A	T	6.2%	205.3 / 3.9	65	T61S (ACT→TCT)	gstA	glutathionine S‑transferase
Reads supporting (aligned to +/- strand):  ref base A (32/29);  new base T (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

CTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCA  >  NC_000913/1714410‑1714704                                                                                                                                                    |                                                                                                                                                    ctcGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTa                                                                                                                                                     >  6:90308/1‑148 (MQ=255)  tcGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTAc                                                                                                                                                    >  7:386083/1‑148 (MQ=255)   cGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGAGTCGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGCTGCCTGCATTGCTGCTGGATGACGGTACt                                                                                                                                                   >  1:370554/1‑148 (MQ=255)     cTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACttt                                                                                                                                                 <  5:90308/148‑1 (MQ=255)       tCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGc                                                                                                                                               >  7:140562/1‑148 (MQ=255)             atCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTAGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGCCGGTACTTTGCTGACg                                                                                                                                          >  3:85600/1‑147 (MQ=255)               cACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACgg                                                                                                                                         >  4:77005/1‑146 (MQ=255)                   cTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCg                                                                                                                                   >  3:220735/1‑148 (MQ=255)                        tGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGa                                                                                                                                        <  4:248868/138‑1 (MQ=255)                        tGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGa                                                                                                                                        >  3:248868/1‑138 (MQ=255)                          agagCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGAt                                                                                                                            <  7:778/148‑1 (MQ=255)                           gagCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCATAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGAt                                                                                                                            >  1:392113/1‑147 (MQ=255)                              cGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATg                                                                                                                        <  2:23083/148‑1 (MQ=255)                               ggAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTTCTTTGCTGACGGAAGGCGTAg                                                                                                                                >  3:252180/1‑139 (MQ=255)                               ggAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTTCTTTGCTGACGGAAGGCGTAg                                                                                                                                >  3:257838/1‑139 (MQ=255)                               ggAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTTCTTTGCTGACGGAAGGCGTAg                                                                                                                                <  4:257838/139‑1 (MQ=255)                               ggAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTTCTTTGCTGACGGAAGGCGTAg                                                                                                                                <  4:252180/139‑1 (MQ=255)                                gAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGATAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGa                                                                                                                                        <  3:375124/130‑1 (MQ=255)                                gAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGa                                                                                                                                        >  4:375124/1‑130 (MQ=255)                                 aaaGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACTGAAGGCGTAGCGATTATGCAg                                                                                                                     >  7:421840/1‑148 (MQ=255)                                  aaGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGTTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTTCTGACGGAAGGCGTAGCGATTATGCAg                                                                                                                     >  1:279449/1‑147 (MQ=255)                                  aaGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGa                                                                                                                                        >  5:33664/1‑128 (MQ=255)                                  aaGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGa                                                                                                                                        <  6:33664/128‑1 (MQ=255)                                   aGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTa                                                                                                                   >  7:218538/1‑148 (MQ=255)                                     gATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATc                                                                                                                 <  7:38950/148‑1 (MQ=255)                                       ttttACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCtt                                                                                                               <  1:402691/148‑1 (MQ=255)                                             ccTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGAc                                                                                                         <  3:77005/148‑1 (MQ=255)                                              cTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGa                                                                                                          >  8:20709/1‑146 (MQ=255)                                                cGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGc                                                                                                      >  6:267052/1‑148 (MQ=255)                                                    aGTGTGTATTTAATGAATAAACTTCTCGAAAACGGTGTCGATTACTTTGCTGTTAAAACTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGGAGCGATTATGCAGTATCTTGCCGACAGCGTc                                                                                                   <  2:279449/147‑1 (MQ=255)                                                            tttAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGa                                                                                                                                        >  4:405930/1‑102 (MQ=255)                                                            tttAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGa                                                                                                                                        <  3:405930/102‑1 (MQ=255)                                                            tttAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGTCAGCGTCCCCGACCGc                                                                                          >  4:230456/1‑148 (MQ=255)                                                            tttAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGc                                                                                          <  6:388782/148‑1 (MQ=255)                                                                aTGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGt                                                                                      <  5:267052/148‑1 (MQ=255)                                                                aTGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGt                                                                                      >  8:33582/1‑148 (MQ=255)                                                                 tGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGtt                                                                                     >  8:355681/1‑148 (MQ=255)                                                                   aaaaaaCGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGTTGACGGTACTTTGCTGACGGAAg                                                                                                                                      <  5:10353/97‑1 (MQ=255)                                                                   aaaaaaCGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGTTGACGGTACTTTGCTGACGGAAg                                                                                                                                      >  6:10353/1‑97 (MQ=255)                                                                   aaaaaaCGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTg                                                                                                                                             <  3:5973/90‑1 (MQ=255)                                                                   aaaaaaCGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTg                                                                                                                                             >  4:5973/1‑90 (MQ=255)                                                                    aaaaaCGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACa                                                                                                        >  7:293660/1‑126 (MQ=255)                                                                    aaaaaCGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTACt                                                                                  <  2:392113/148‑1 (MQ=255)                                                                       aaCGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTgg                                                                                >  5:353247/1‑147 (MQ=255)                                                                                            ataaCTTTGCCGTTAACCCTAAGGGGCAGGAGCCTGCATTGCTGCTGGATGACGGTACATAGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGCCCGCCAGTTGCAGGCACCGGTAAACAGTATTTCCCg                                                          >  3:69653/4‑148 (MQ=255)                                                                                                  ttGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCtata                                                     >  4:148628/1‑147 (MQ=255)                                                                                                   tGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATaa                                                    <  3:148628/147‑1 (MQ=255)                                                                                                           aCCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGAc                                                                                                         <  8:448493/86‑1 (MQ=255)                                                                                                           aCCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGAc                                                                                                         >  7:448493/1‑86 (MQ=255)                                                                                                           aCCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGa                                           <  7:20709/148‑1 (MQ=255)                                                                                                           aCCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGa                                           <  4:85600/148‑1 (MQ=255)                                                                                                           aCCCTAAGGGGCAGGGGCCTGCATTGCTGCTGGATGACGGTACTTTGCGGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGa                                           <  2:370554/148‑1 (MQ=255)                                                                                                                    ggCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGaa                                  <  8:421840/148‑1 (MQ=255)                                                                                                                         gTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGAGTATGGAGTATCTTGCCGACCGCGTCGCCGACCGCCAGTAGATGTCACCGGTACACAGTATTTCCCGCTATAACACCAGCGAAGGTCTGAATTACa                             >  4:292796/1‑148 (MQ=255)                                                                                                                          tGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACAt                            <  7:33582/148‑1 (MQ=255)                                                                                                                           gCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGt                                                                  <  3:141257/109‑1 (MQ=255)                                                                                                                           gCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGt                                                                  >  4:141257/1‑109 (MQ=255)                                                                                                                               gCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCa                       >  6:120991/1‑148 (MQ=255)                                                                                                                                 aTTGATGCTGGATGACGGTACTTTGCTGACTGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCAcc                     <  4:220735/148‑1 (MQ=255)                                                                                                                                   tgctgcTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATc                                             <  6:156723/122‑1 (MQ=255)                                                                                                                                   tgctgcTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATc                                             >  5:156723/1‑122 (MQ=255)                                                                                                                                   tgctgcTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCAcc                     >  8:210203/1‑146 (MQ=255)                                                                                                                                           gATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCAt            >  7:218632/1‑147 (MQ=255)                                                                                                                                                 ggTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGtt     <  8:218538/148‑1 (MQ=255)                                                                                                                                                 ggTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGtt     <  7:355681/148‑1 (MQ=255)                                                                                                                                                   tACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGTCAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTc   <  3:230456/148‑1 (MQ=255)                                                                                                                                                    aCTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTca  >  3:127854/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTACCCTCGTCAGTGTGGATTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACAGCGTCCCCGACCGCCAGTTGCTGGCACCGGTAAACAGTATTTCCCGCTATAAAACCATCGAATGGCTGAATTACATCGCCACCGAGCTGCATAAAGGTTTCA  >  NC_000913/1714410‑1714704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑