Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,775,006 C→T 5.2% S107S (AGC→AGT aroD → 3‑dehydroquinate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,775,0060CT 5.2% 251.6 / 2.5 77S107S (AGC→AGTaroD3‑dehydroquinate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (33/40);  new base T (2/2);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

GGAGTCTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCAT  >  NC_000913/1774859‑1775149
                                                                                                                                                   |                                                                                                                                               
ggAGTCTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGc                                                                                                                                                 >  2:374292/1‑148 (MQ=255)
        tCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCt                                                                                                                                            <  3:100150/145‑1 (MQ=255)
        tCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTg                                                                                                                                           <  7:202900/146‑1 (MQ=255)
         cATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCAACGACAGCGGCCTGGtt                                                                                                                                        >  1:60353/1‑148 (MQ=255)
          aTGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGTTTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTg                                                                                                                                       <  5:246687/148‑1 (MQ=255)
             gcggcAGCAAAAATTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGat                                                                                                                                     <  8:285751/147‑1 (MQ=255)
             gcggcAGCAAAAATTCTCCGTGAGACCATGCCAGAAAAACCGATGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGat                                                                                                                                     <  5:400995/147‑1 (MQ=255)
                   gcaAAAATTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATgatcg                                                                                                                              <  2:195787/148‑1 (MQ=255)
                     aaaaaTTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGat                                                                                                                                     >  8:79672/1‑139 (MQ=255)
                     aaaaaTTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGat                                                                                                                                     <  7:79672/139‑1 (MQ=255)
                     aaaaaTTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATgatcgat                                                                                                                            >  3:409585/1‑148 (MQ=255)
                     aaaaaTTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATgatcgat                                                                                                                            <  6:141147/148‑1 (MQ=255)
                        aaTTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTg                                                                                                                         <  3:116476/148‑1 (MQ=255)
                             tcCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTg                                                                                                                                           >  7:176582/1‑125 (MQ=255)
                             tcCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTg                                                                                                                                           <  8:176582/125‑1 (MQ=255)
                               cGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGt                                                                                                                     <  7:435174/145‑1 (MQ=255)
                               cGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTa                                                                                                                   <  7:374398/147‑1 (MQ=255)
                                      ccATGCCAGAAAAACCGCTGCTGTTTACCTTCAGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATgatcga                                                                                                                             <  7:305081/130‑1 (MQ=255)
                                      ccATGCCAGAAAAACCGCTGCTGTTTACCTTCAGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATgatcga                                                                                                                             >  8:305081/1‑130 (MQ=255)
                                           ccAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGc                                                                                                                                              >  2:144532/1‑108 (MQ=255)
                                           ccAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGc                                                                                                                                              <  1:144532/108‑1 (MQ=255)
                                               aaaaaCCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGTGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTgatgat                                                                                                    >  6:27726/1‑146 (MQ=255)
                                               aaaaaCCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGTGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTgatgat                                                                                                    >  6:154052/1‑146 (MQ=255)
                                                aaaaCCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAg                                                                                                 >  4:69611/1‑148 (MQ=255)
                                                aaaaCCGATGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGACGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGATATTTAACGGTGATGATCAg                                                                                                 >  5:217059/1‑148 (MQ=255)
                                                  aaCCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCTAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGt                                                                                               <  1:381178/148‑1 (MQ=255)
                                                   aCCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGt                                                                                               >  6:348373/1‑147 (MQ=255)
                                                            gTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTgg                                                                                                                                          >  6:165063/1‑95 (MQ=255)
                                                            gTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTgg                                                                                                                                          <  5:165063/95‑1 (MQ=255)
                                                            gTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAAcc                                                                                     >  2:89500/1‑148 (MQ=255)
                                                              ttACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGaaa                                                                                       >  7:1059/1‑144 (MQ=255)
                                                              ttACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGaaa                                                                                       <  8:1059/144‑1 (MQ=255)
                                                               tACCTTCCGCAGTTCCAAAGAAGGCGGCTAGCAGGCTATTTCCTCCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCTGCCTGGTTGATATGATCGATCTGTAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGt                                                                                   <  2:60353/147‑1 (MQ=255)
                                                               tACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGt                                                                                   <  3:69611/147‑1 (MQ=255)
                                                                 ccTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCg                                                                                 <  4:409585/147‑1 (MQ=255)
                                                                        cAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGcc                                                                         >  8:226125/1‑148 (MQ=255)
                                                                         aGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGcc                                                                         >  1:399775/1‑147 (MQ=255)
                                                                              cAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGtgatga                                                                                                     >  8:312384/1‑114 (MQ=255)
                                                                              cAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGtgatga                                                                                                     <  7:312384/114‑1 (MQ=255)
                                                                               aaaGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATc                                                                                                   <  3:51503/115‑1 (MQ=255)
                                                                               aaaGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATc                                                                                                   >  4:51503/1‑115 (MQ=255)
                                                                                gagaagGCGGCGAGCAGGCGATTTCCACCGAGGCTTAGATTGCACTCAATCGTGCCGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTAGTTACCGGTGATGAGCAGGTTAAAGAAACCGTCGCCTACGCCCAcgcg                                                                   >  6:411350/2‑146 (MQ=255)
                                                                                 agaagGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCat                                                                <  1:374292/148‑1 (MQ=255)
                                                                                   aagGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCatga                                                              <  2:399775/148‑1 (MQ=255)
                                                                                       cggcgAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGGTATGATCGATCTGGAGTTAtt                                                                                                                 <  5:206382/93‑1 (MQ=255)
                                                                                       cggcgAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGGTATGATCGATCTGGAGTTAtt                                                                                                                 >  6:206382/1‑93 (MQ=255)
                                                                                       cggcgAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACgcgc                                                                  >  4:22203/1‑140 (MQ=255)
                                                                                       cggcgAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACgcgc                                                                  <  3:22203/140‑1 (MQ=255)
                                                                                         gcgAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGaa                                                        <  5:348373/148‑1 (MQ=255)
                                                                                            aGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAagt                                                     <  1:89500/148‑1 (MQ=255)
                                                                                               aGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAagtagt                                                  <  7:226125/148‑1 (MQ=255)
                                                                                                  cGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTaa                                                                                            <  8:437042/103‑1 (MQ=255)
                                                                                                  cGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTaa                                                                                            >  7:437042/1‑103 (MQ=255)
                                                                                                   gATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATg                                              >  1:426455/1‑148 (MQ=255)
                                                                                                    tttttCACCGAGGCTTATTTTGCTCTCTATCGTGCAGCCATCTACAGCGGCCTGGTTGTTATGATCGATCTGGAGTTATTTTCCGGTTATGATCAGGTTATAGTAACCGTCGCCTACGCCCACGCGCATGATGTGAATGTATTCATGt                                             <  6:217059/147‑1 (MQ=255)
                                                                                                     tttCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTc                                            >  4:233491/1‑148 (MQ=255)
                                                                                                         cACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGTGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCaa                                         <  5:154052/147‑1 (MQ=255)
                                                                                                         cACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGTGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCaa                                         <  5:27726/147‑1 (MQ=255)
                                                                                                           ccGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCa                                      <  2:426455/148‑1 (MQ=255)
                                                                                                              aGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGa                                   <  3:233491/148‑1 (MQ=255)
                                                                                                              aGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGa                                   >  5:29905/1‑148 (MQ=255)
                                                                                                                   tataTTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGaaa                                                                                       <  8:129711/91‑1 (MQ=255)
                                                                                                                   tataTTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGaaa                                                                                       >  7:129711/1‑91 (MQ=255)
                                                                                                                    atatTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGaa                                                        >  1:213489/1‑121 (MQ=255)
                                                                                                                    atatTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGaa                                                        <  2:213489/121‑1 (MQ=255)
                                                                                                                     tatTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGAtgtg                                                          <  6:287847/118‑1 (MQ=255)
                                                                                                                     tatTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGAtgtg                                                          >  5:287847/1‑118 (MQ=255)
                                                                                                                        tGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATaa                          >  5:49428/1‑147 (MQ=255)
                                                                                                                          cACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACgcgc                                                                  >  7:143684/1‑105 (MQ=255)
                                                                                                                          cACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACgcgc                                                                  <  8:143684/105‑1 (MQ=255)
                                                                                                                              cAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCa                                                >  7:403528/1‑119 (MQ=255)
                                                                                                                              cAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCa                                                <  8:403528/119‑1 (MQ=255)
                                                                                                                                      cAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCg            >  2:428902/1‑147 (MQ=255)
                                                                                                                                        gCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCg            >  8:158952/1‑145 (MQ=255)
                                                                                                                                             cGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAAt     >  6:200015/1‑147 (MQ=255)
                                                                                                                                               aCAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCAt  <  6:49428/148‑1 (MQ=255)
                                                                                                                                                  gCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGcc                    >  2:421441/1‑127 (MQ=255)
                                                                                                                                                  gCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGcc                    <  1:421441/127‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GGAGTCTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATATTGCACTCAATCGTGCAGCCATCGACAGCGGCCTGGTTGATATGATCGATCTGGAGTTATTTACCGGTGATGATCAGGTTAAAGAAACCGTCGCCTACGCCCACGCGCATGATGTGAAAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCAT  >  NC_000913/1774859‑1775149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: