Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,219,647 T→C 5.3% intergenic (‑166/+45) osmF ← / ← bglX putative ABC transporter periplasmic binding protein/beta‑D‑glucoside glucohydrolase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,219,6470TC 5.3% 297.9 / 5.2 94intergenic (‑166/+45)osmF/bglXputative ABC transporter periplasmic binding protein/beta‑D‑glucoside glucohydrolase, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (36/53);  new base C (2/3);  total (38/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

ACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGATTCC  >  NC_000913/2219502‑2219792
                                                                                                                                                 |                                                                                                                                                 
aCTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTa                                                                                                                                                  <  2:5378/147‑1 (MQ=255)
 cTGTTGTTTTCAGCGAATTAAGATAAAAGCATAGTTGATAATGGCGGGGTTAGGTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATc                                                                                                                                                <  2:175741/148‑1 (MQ=255)
 cTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATc                                                                                                                                                <  8:51960/148‑1 (MQ=255)
   gttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCg                                                                                                                                              >  8:293817/1‑148 (MQ=255)
   gttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCg                                                                                                                                              >  3:134843/1‑148 (MQ=255)
    ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCgg                                                                                                                                             >  7:455720/1‑148 (MQ=255)
    ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCgg                                                                                                                                             <  2:273913/148‑1 (MQ=255)
    ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATAAGGCGCTCGCGCCGCATcc                                                                                                                                               <  1:319818/146‑1 (MQ=255)
    ttgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATAAGGCGCTCGCGCCGCATcc                                                                                                                                               >  2:319818/1‑146 (MQ=255)
     tgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCgg                                                                                                                                             <  3:11383/147‑1 (MQ=255)
     tgttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCgg                                                                                                                                             >  4:11383/1‑147 (MQ=255)
      gttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGc                                                                                                                                            <  2:164490/147‑1 (MQ=255)
      gttTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATAAGGCGCTCGCGCCGCATCCGGc                                                                                                                                            <  6:429098/147‑1 (MQ=255)
            aGCGAATTAAGTGAAAAGCTTAGTTGATATTGGCTGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCATATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACaa                                                                                                                                      <  8:305990/147‑1 (MQ=39)
            aGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACaa                                                                                                                                      <  3:398390/147‑1 (MQ=255)
            aGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCGTATCCGGCCTACaa                                                                                                                                      <  1:297861/147‑1 (MQ=255)
               gAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAggg                                                                                                                                  >  8:366597/1‑148 (MQ=255)
               gAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAggg                                                                                                                                  <  3:226803/148‑1 (MQ=255)
               gAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAggg                                                                                                                                  <  1:212889/148‑1 (MQ=255)
                aaTTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGgc                                                                                                                                 <  3:5405/148‑1 (MQ=255)
                 aTTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGgcg                                                                                                                                <  7:293817/148‑1 (MQ=255)
                    aaGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCa                                                                                                                              >  8:456132/1‑147 (MQ=255)
                     agagAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCaa                                                                                                                             <  4:348019/147‑1 (MQ=255)
                      gagaAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAAc                                                                                                                           >  7:448935/1‑148 (MQ=255)
                            aGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTcgcg                                                                                                                     <  8:448935/148‑1 (MQ=255)
                                 aGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACa                                                                                                                                       >  4:244658/1‑125 (MQ=255)
                                 aGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACa                                                                                                                                       <  3:244658/125‑1 (MQ=255)
                                 aGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCt                                                                                                                 >  4:236938/1‑147 (MQ=255)
                                  gTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTa                                                                                                               >  6:282713/1‑148 (MQ=255)
                                  gTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTa                                                                                                               >  6:99267/1‑148 (MQ=255)
                                    tGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTAc                                                                                                              >  3:188474/1‑147 (MQ=255)
                                     gATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACt                                                                                                             <  8:271562/147‑1 (MQ=255)
                                      atcaTGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTaa                                                                                                           <  3:23370/145‑1 (MQ=255)
                                      aTAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTaa                                                                                                           <  7:454432/148‑1 (MQ=255)
                                      aTAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTa                                                                                                            <  7:456132/147‑1 (MQ=255)
                                        aaTGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTTAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAg                                                                                                         >  1:384746/1‑148 (MQ=255)
                                         aTGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAg                                                                                                         >  7:225555/1‑147 (MQ=255)
                                         aTGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAg                                                                                                         <  4:188474/147‑1 (MQ=255)
                                         aTGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGc                                                                                                        <  3:236938/148‑1 (MQ=255)
                                           ggCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCAt                                                                                                      >  7:328225/1‑148 (MQ=255)
                                              ggggTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTa                                                                                                    >  5:350886/1‑147 (MQ=255)
                                               gggTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCACTTACTAAAGCATTa                                                                                                    >  4:92335/1‑146 (MQ=255)
                                                   tAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCt                                                                                                                                          >  1:84723/1‑104 (MQ=255)
                                                   tAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCt                                                                                                                                          <  2:84723/104‑1 (MQ=255)
                                                   tAGCTTGAGCCGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCaa                                                                                              >  4:402660/1‑148 (MQ=255)
                                                     gCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATTAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACt                                                                                            <  3:129879/148‑1 (MQ=255)
                                                      cTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTc                                                                                           <  4:67666/148‑1 (MQ=255)
                                                       ttGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTc                                                                                           >  1:429865/1‑147 (MQ=255)
                                                        tGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCa                                                                                          >  1:180525/1‑147 (MQ=255)
                                                        tGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCa                                                                                          >  2:184564/1‑147 (MQ=255)
                                                                 ggATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCtt                                                                                <  7:366597/148‑1 (MQ=255)
                                                                 ggATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCAGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCtt                                                                                <  8:78321/148‑1 (MQ=255)
                                                                  gATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCttt                                                                               <  8:341207/148‑1 (MQ=255)
                                                                  gATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCttt                                                                               <  8:328225/148‑1 (MQ=255)
                                                                      cAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCtt                                                                            >  1:106622/1‑147 (MQ=255)
                                                                       aGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTaa                                                                          <  8:455720/148‑1 (MQ=255)
                                                                       aGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTa                                                                           <  5:99267/147‑1 (MQ=255)
                                                                        gTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTAc                                                                                                   >  2:160199/1‑122 (MQ=255)
                                                                        gTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTAc                                                                                                   <  1:160199/122‑1 (MQ=255)
                                                                         ttGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCTCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAAcg                                                                        <  4:91017/148‑1 (MQ=255)
                                                                                aTCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGAGTgag                                                                  >  3:89142/1‑145 (MQ=255)
                                                                                    gATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATc                                                             <  2:83957/148‑1 (MQ=255)
                                                                                    gATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATc                                                             <  2:348461/148‑1 (MQ=255)
                                                                                     aTAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCa                                                            <  2:429865/148‑1 (MQ=255)
                                                                                     aTAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCa                                                            <  3:402660/148‑1 (MQ=255)
                                                                                         aTACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCa                                                            <  2:328703/144‑1 (MQ=255)
                                                                                         aTACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCa                                                            >  1:328703/1‑144 (MQ=255)
                                                                                           aCTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGcc                                                       >  7:387868/1‑147 (MQ=255)
                                                                                              tAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGAt                                                    >  8:409550/1‑147 (MQ=255)
                                                                                                agagGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATaa                                                  <  8:225555/147‑1 (MQ=255)
                                                                                                    gCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCt                                                                                                                                          >  2:315083/1‑55 (MQ=255)
                                                                                                    gCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCt                                                                                                                                          <  1:315083/55‑1 (MQ=255)
                                                                                                        atTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTg                                          >  2:391559/1‑147 (MQ=255)
                                                                                                        atTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTg                                          >  5:269387/1‑147 (MQ=255)
                                                                                                         ttCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGaa                                        <  1:66670/148‑1 (MQ=255)
                                                                                                         ttCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGa                                         <  6:269387/147‑1 (MQ=255)
                                                                                                         ttCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGa                                         <  1:391559/147‑1 (MQ=255)
                                                                                                              tCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAAc                                       >  3:214790/1‑144 (MQ=255)
                                                                                                              tCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAAc                                       <  4:214790/144‑1 (MQ=255)
                                                                                                                cGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGGCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGcc                                  <  4:134843/147‑1 (MQ=255)
                                                                                                                 ggCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCtt                                                                                                                >  6:130387/1‑68 (MQ=255)
                                                                                                                 ggCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCtt                                                                                                                <  5:130387/68‑1 (MQ=255)
                                                                                                                         aggCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAAc                                       <  7:260575/131‑1 (MQ=39)
                                                                                                                         aggCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAAc                                       >  8:260575/3‑133 (MQ=39)
                                                                                                                            ccGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCACTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTc                      <  3:92335/147‑1 (MQ=255)
                                                                                                                                  gcggcgCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATAttt                >  5:281838/1‑147 (MQ=255)
                                                                                                                                      cgcgAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTg                                    <  5:308509/123‑1 (MQ=255)
                                                                                                                                      cgcgAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTg                                    >  6:308509/1‑123 (MQ=255)
                                                                                                                                       gcgAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTg          <  2:384746/148‑1 (MQ=255)
                                                                                                                                         gAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGtt        <  2:203401/148‑1 (MQ=255)
                                                                                                                                          aGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTg       <  2:106622/148‑1 (MQ=255)
                                                                                                                                            cgcCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGAt     <  8:387868/148‑1 (MQ=255)
                                                                                                                                             gcCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGAtt    >  4:143032/1‑148 (MQ=255)
                                                                                                                                               cTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGATTcc  <  6:281838/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTGAGCGGAAGGATTCAGTTGCAGAATCAGATAAATACTTAAGAGGCATATTCGGTCCGGCGTTTCCTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAAAGGGCGCAAACGTCGCGCCCTTACTAAAGCATTACAGCAACTCAAACTCGCCTTTCTTAACGCGTGCGGAATCAGTGCCGATAAAGACATTGAACTTGCCAGGCTCGGCGTCATATTTCATCTGTTGATTCC  >  NC_000913/2219502‑2219792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: