Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,336,597 T→C 5.0% V16V (GTA→GTG)  yfaA ← DUF2138 family protein, putative host defense protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,336,5970TC 5.0% 251.5 / 2.6 79V16V (GTA→GTG) yfaADUF2138 family protein, putative host defense protein
Reads supporting (aligned to +/- strand):  ref base T (39/36);  new base C (2/2);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

GTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTT  >  NC_000913/2336450‑2336744
                                                                                                                                                   |                                                                                                                                                   
gTCGCTGGCGAGAAAGAGGTCCGGCTCATTGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCt                                                                                                                                                     <  1:275188/148‑1 (MQ=255)
   gCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACa                                                                                                                                                  <  2:152147/148‑1 (MQ=255)
     tGTCGAGAAAGAGGTCCGGCTCATTGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTAc                                                                                                                                                   >  5:421689/1‑145 (MQ=255)
        cGAGAAAGAGGTCCGGCTCATTGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGAc                                                                                                                                              >  7:437796/1‑147 (MQ=255)
         gagaAATAGTTCCGGCTAATTGATATATATTTGAATATTGTTGTGCACCACCATCTGCGACAACTTTTTTTCTGGCCCACTGCCAGCATATTGCAACTCCGAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCa                                                                                                                                            <  2:73212/148‑1 (MQ=255)
          agaAAGAGGTCCGGCTCATTGAGATCGATTTGCAGATTGTTGTTCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCAAGCATATAGCAACGCCCCGACGCCAGCGGAAAGCAGTGCTATTGCTCCAAAACCTACCAGACCa                                                                                                                                            >  6:90462/1‑147 (MQ=255)
          agaAAGAGGTCCGGCTCATTGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCa                                                                                                                                            >  5:103787/1‑147 (MQ=255)
           gaAAGAGGTCCGGCTCATTGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCa                                                                                                                                            >  7:154450/1‑146 (MQ=255)
               gagGTCCGGCTCATTGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAAc                                                                                                                                      >  6:254915/1‑148 (MQ=255)
                           aTTGAGATCGATTTGCAGATTGTTGTGCACAACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCttt                                                                                                                           <  5:251577/147‑1 (MQ=255)
                            ttGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGaaa                                                                                                                                      <  2:446005/135‑2 (MQ=255)
                            ttGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGaaa                                                                                                                                      >  1:446005/1‑134 (MQ=255)
                            ttGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCg                                                                                                                         <  6:103787/148‑1 (MQ=255)
                            ttGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCg                                                                                                                         >  4:385728/1‑148 (MQ=255)
                            ttGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCg                                                                                                                         >  7:121347/1‑148 (MQ=255)
                             tGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACAATAGAACCGCCAGCCTTTCGc                                                                                                                        >  5:325763/1‑148 (MQ=255)
                              gagaTCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGcc                                                                                                                       >  7:452719/1‑148 (MQ=255)
                                  tCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCtttt                                                                                                                   >  7:324508/1‑148 (MQ=255)
                                   cGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCttttt                                                                                                                  >  7:203785/1‑148 (MQ=255)
                                        tGCAGATTGTGGTGCACCACCAGCGGCGACACCGTTTTTTCTGGCCCCCTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTc                                                                                                               <  2:443744/146‑1 (MQ=255)
                                          cAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTcacca                                                                                                           >  3:165139/1‑148 (MQ=255)
                                            gATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACt                                                                                                         >  1:437270/1‑148 (MQ=255)
                                             aTTGTTGTGCTCCCCCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCATCATATTGCAAAGCCCAGACTCCAGCGGAATTCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGACTTTTTTTCACCACTc                                                                                                        <  6:325763/148‑1 (MQ=255)
                                             aTTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACt                                                                                                         >  6:145886/1‑147 (MQ=255)
                                              ttgttgTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCa                                                                                                       <  8:121266/148‑1 (MQ=255)
                                                    tgCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGcc                                                                                                  <  3:132777/147‑1 (MQ=255)
                                                      caccacCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCCTTTTTTCACCACTCATTGCcac                                                                                                <  6:421689/147‑1 (MQ=255)
                                                         caccaGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTc                                                                                            <  3:190100/148‑1 (MQ=255)
                                                           ccaGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCt                                                                                          >  8:244056/1‑148 (MQ=255)
                                                           ccaGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCt                                                                                          <  5:254915/148‑1 (MQ=255)
                                                            caGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCttttttt                                                                                                                >  6:75288/1‑125 (MQ=255)
                                                            caGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCttttttt                                                                                                                <  5:75288/125‑1 (MQ=255)
                                                             aGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTc                                                                                                        <  4:67936/132‑1 (MQ=255)
                                                             aGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTc                                                                                                        >  3:67936/1‑132 (MQ=255)
                                                             aGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCCACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGcc                                                                                                  >  6:416862/1‑138 (MQ=255)
                                                             aGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCCACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGcc                                                                                                  <  5:416862/138‑1 (MQ=255)
                                                                 gcgACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGta                                                                                    <  5:145886/148‑1 (MQ=255)
                                                                    aCAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCCACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATag                                                                                 >  7:416533/1‑148 (MQ=255)
                                                                     cAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTc                                                                                                               <  5:3642/117‑1 (MQ=255)
                                                                     cAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTc                                                                                                               >  6:3642/1‑117 (MQ=255)
                                                                         gTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATagccagc                                                                            >  5:321891/1‑148 (MQ=255)
                                                                          ttttttCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATagccagc                                                                            >  5:243118/1‑147 (MQ=255)
                                                                           tttttCTGGCCCTCTCCCCGCATAGTGCAACGACCAGACGCCACCGGAAAGCAGTGCGATTGCGCCAAAACCTACAAGACCATAGAACCTCCAGCCTTTCGCC‑TTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGACATCCAt                                                                         >  4:25835/1‑148 (MQ=255)
                                                                            ttttCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAgccagcca                                                                          <  2:437270/147‑1 (MQ=255)
                                                                                    cccACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACg                                                                 <  7:244056/148‑1 (MQ=255)
                                                                                       aCTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCGTGCGCCTTTTTTTCCCCACTCATGGCCACATGCCTTGTGTATAGCCAGCCATTTTTTACGGGc                                                              >  7:321404/1‑148 (MQ=255)
                                                                                        cTGCCATCATATTGCAACGCCCTTACGCCTGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCTCCAGGCTTTCGCCTTTTTTTCACCACTCATTGCCACTTTCCTTGTGTATAGCCAGCCATTTTTTACGGGca                                                             <  3:115447/148‑1 (MQ=255)
                                                                                           ccAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGcaca                                                           <  6:243118/147‑1 (MQ=255)
                                                                                            cAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGc                                                         >  2:176210/1‑148 (MQ=255)
                                                                                             aGCATATTCCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGc                                                         >  3:152817/1‑147 (MQ=255)
                                                                                               cATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCcaca                                                                                               >  4:167955/1‑107 (MQ=255)
                                                                                               cATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCcaca                                                                                               <  3:167955/107‑1 (MQ=255)
                                                                                               cATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCaa                                                      <  8:437796/148‑1 (MQ=255)
                                                                                                  atTGCCACGCCCATACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACt                                                   >  2:284425/1‑148 (MQ=255)
                                                                                                  atTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTtgt                                                                                       >  3:15132/1‑112 (MQ=255)
                                                                                                  atTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTtgt                                                                                       <  4:15132/112‑1 (MQ=255)
                                                                                                    tGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCAtttttt                                                                    >  8:430496/1‑129 (MQ=255)
                                                                                                    tGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCAtttttt                                                                    <  7:430496/129‑1 (MQ=255)
                                                                                                    tGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGACAAACtt                                                  >  6:79210/1‑147 (MQ=255)
                                                                                                      cAACGCCCAGACGCGAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTAc                                               >  2:183773/1‑148 (MQ=255)
                                                                                                      cAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTa                                                <  6:321891/147‑1 (MQ=255)
                                                                                                      cAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTa                                                <  5:171740/147‑1 (MQ=255)
                                                                                                        aCGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCg                                             >  8:13317/1‑148 (MQ=255)
                                                                                                           cccAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCtt                                                                                         >  2:143285/1‑101 (MQ=255)
                                                                                                           cccAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCtt                                                                                         <  1:143285/101‑1 (MQ=255)
                                                                                                           cccAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCCACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGc                                          <  8:416533/148‑1 (MQ=255)
                                                                                                             ccgccGCCAGCGGAAAGCAGTGCTATAGCGCCAAAACCTACAAGACCAAAGAACCGCCAGCCATTCGCCTTTTTTTCACCACGCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGccc                                        <  5:90462/144‑1 (MQ=255)
                                                                                                             cAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCCGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGccc                                        >  5:123398/1‑148 (MQ=255)
                                                                                                                aCGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTaa                                     <  4:165139/148‑1 (MQ=255)
                                                                                                                                gTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAg                     <  3:385728/148‑1 (MQ=255)
                                                                                                                                  gCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTtg                                                                                        <  6:231621/79‑1 (MQ=255)
                                                                                                                                  gCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTtg                                                                                        >  5:231621/1‑79 (MQ=255)
                                                                                                                                      ttGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTg               <  8:121347/148‑1 (MQ=255)
                                                                                                                                      ttGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTg               <  4:152817/148‑1 (MQ=255)
                                                                                                                                            cAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCAt                                                                         <  8:177113/84‑1 (MQ=255)
                                                                                                                                            cAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCAt                                                                         >  7:177113/1‑84 (MQ=255)
                                                                                                                                             aaaaCCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGccc                       >  7:344435/1‑133 (MQ=255)
                                                                                                                                             aaaaCCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGccc                       <  8:344435/133‑1 (MQ=255)
                                                                                                                                             aaaaCCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTg        >  8:199869/1‑148 (MQ=255)
                                                                                                                                               aaCCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGa      <  8:452719/148‑1 (MQ=255)
                                                                                                                                                   tACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACttt  >  7:320077/1‑148 (MQ=255)
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GTCGCTGTCGAGAAAGAGGTCCGGCTCATTGAGATCGATTTGCAGATTGTTGTGCACCACCAGCGGCGACAACGTTTTTTCTGGCCCACTGCCAGCATATTGCAACGCCCAGACGCCAGCGGAAAGCAGTGCTATTGCGCCAAAACCTACAAGACCATAGAACCGCCAGCCTTTCGCCTTTTTTTCACCACTCATTGCCACATTCCTTGTGTATAGCCAGCCATTTTTTACGGGCACAGCCAAACTTTACCGTGCCCTAATACGACAAAAGCCCAGACTTTGCAGCCTGGACTTT  >  NC_000913/2336450‑2336744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑