Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,396,264 A→C 5.0% C63G (TGT→GGT)  nuoI ← NADH:ubiquinone oxidoreductase, chain I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,396,2640AC 5.0% 257.1 / 5.2 80C63G (TGT→GGT) nuoINADH:ubiquinone oxidoreductase, chain I
Reads supporting (aligned to +/- strand):  ref base A (37/39);  new base C (2/2);  total (39/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

TGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAAC  >  NC_000913/2396117‑2396411
                                                                                                                                                   |                                                                                                                                                   
tGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACa                                                                                                                                                     <  8:234756/148‑1 (MQ=255)
  gTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAg                                                                                                                                                    <  1:16851/147‑1 (MQ=255)
        cAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACg                                                                                                                                              >  7:344584/1‑147 (MQ=255)
            cttcttCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACg                                                                                                                                         >  6:388893/1‑148 (MQ=255)
                 tcGCACAGCCCACAGAAAATGCAGCGTGAGAAGTTAATGCGGAAAAATACCGGGTACCACAGACCGTCTTTCGTATCTCCATTTTGAAGCGAGATACAGCCGACCGTGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCtt                                                                                                                                    >  1:118293/1‑148 (MQ=255)
                  cGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGctct                                                                                                                                     <  8:344584/146‑1 (MQ=255)
                   gCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTc                                                                                                                                   <  5:388893/147‑1 (MQ=255)
                      caGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCTCAGAGGTTACAGGCTACGCAACGCTCTTCGCCg                                                                                                                               <  2:1032/148‑1 (MQ=255)
                      caGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCg                                                                                                                               >  3:404291/1‑148 (MQ=255)
                      caGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCg                                                                                                                               >  2:253637/1‑148 (MQ=255)
                       aGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACCGGCTACGCAACGCTCTTCGCCGt                                                                                                                              >  2:416104/1‑148 (MQ=255)
                       aGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACCGGCTACGCAACGCTCTTCGCCGt                                                                                                                              >  8:222861/1‑148 (MQ=255)
                        gACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTc                                                                                                                             <  5:340639/148‑1 (MQ=255)
                              aGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTc                                                                                                                       <  5:381247/148‑1 (MQ=255)
                              aGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTc                                                                                                                       >  5:395916/1‑148 (MQ=255)
                                 aaaTGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTcgcg                                                                                                                    >  2:137668/1‑148 (MQ=255)
                                   aTGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCggg                                                                                                                  >  5:221902/1‑148 (MQ=255)
                                   aTGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCggg                                                                                                                  <  8:297794/148‑1 (MQ=255)
                                        gCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCATTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGa                                                                                                             <  5:238518/148‑1 (MQ=255)
                                           tGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACg                                                                                                          <  6:395916/148‑1 (MQ=255)
                                            gagaAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTACGCCGTCCGGGTCGCGGGTCAGAACGa                                                                                                         >  5:399424/1‑148 (MQ=255)
                                             agaAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACCGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGAt                                                                                                        <  7:222861/148‑1 (MQ=255)
                                             agaAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACCGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGAt                                                                                                        <  1:416104/148‑1 (MQ=255)
                                             agaAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGAt                                                                                                        <  6:221902/148‑1 (MQ=255)
                                              gaAGTTGATGAGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGATTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGAt                                                                                                        <  6:86912/147‑1 (MQ=255)
                                                  ttGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGa                                                                                                             >  8:166787/1‑138 (MQ=255)
                                                  ttGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGa                                                                                                             <  7:166787/138‑1 (MQ=255)
                                                  ttGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTCGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTCAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGAcc                                                                                                  <  2:393719/148‑1 (MQ=255)
                                                   tGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTcc                                                                                                                            >  6:206740/1‑122 (MQ=255)
                                                   tGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTcc                                                                                                                            <  5:206740/122‑1 (MQ=255)
                                                     aTGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCa                                                                                                 <  1:253637/147‑1 (MQ=255)
                                                      tGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGAAACGACCACg                                                                                               <  3:331771/148‑1 (MQ=255)
                                                         ggAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCt                                                                                                                                                 >  8:141392/1‑95 (MQ=255)
                                                         ggAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCt                                                                                                                                                 <  7:141392/95‑1 (MQ=255)
                                                           aaaaaTTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAAc                                                                                          <  1:137668/148‑1 (MQ=255)
                                                                     ggTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGggcgg                                                                                   >  8:378216/1‑145 (MQ=255)
                                                                      gTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGa                                                                               <  4:404291/148‑1 (MQ=255)
                                                                       tACCAGCGCGCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGTTTACAGGCTTCGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATATCGGGGCGGCAGAt                                                                              <  2:118293/148‑1 (MQ=255)
                                                                         ccAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGa                                                                               >  2:128612/1‑145 (MQ=255)
                                                                           aGCGAACGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGTCAGGCTACCGCGCAGAGGTTACAGGCTACCCAACGCTCTTCGCCGTCCGGGACGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGAc                                                                          >  6:310988/1‑148 (MQ=255)
                                                                             cGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCg                                                                        >  5:311313/1‑148 (MQ=255)
                                                                              gACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCgg                                                                       <  4:123716/148‑1 (MQ=255)
                                                                                    cTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTc                                                                 >  8:270603/1‑148 (MQ=255)
                                                                                     tttGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGa                                                                                                         <  7:214976/107‑1 (MQ=255)
                                                                                     tttGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGa                                                                                                         >  8:214976/1‑107 (MQ=255)
                                                                                     tttGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGggc                                                                                     >  5:303666/1‑127 (MQ=255)
                                                                                     tttGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGggc                                                                                     <  6:303666/127‑1 (MQ=255)
                                                                                      ttGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCg                                                               >  8:181195/1‑148 (MQ=255)
                                                                                           ttCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTa                                                           >  2:419000/1‑147 (MQ=255)
                                                                                            tCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACgg                                                                                        <  1:376980/117‑1 (MQ=255)
                                                                                            tCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACgg                                                                                        >  2:376980/1‑117 (MQ=255)
                                                                                             cTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACAt                                                        >  7:427616/1‑148 (MQ=255)
                                                                                             cTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACAt                                                        >  3:140933/1‑148 (MQ=255)
                                                                                               gCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACg                                                                                         >  3:256752/1‑113 (MQ=255)
                                                                                               gCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACg                                                                                         <  4:256752/113‑1 (MQ=255)
                                                                                                cTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACAttcg                                                     >  5:202046/1‑148 (MQ=255)
                                                                                                cTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACAtt                                                       <  6:311313/146‑1 (MQ=255)
                                                                                                   tttGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACAttcgcgt                                                  >  1:377872/1‑148 (MQ=255)
                                                                                                       cAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCACTTCGCCTTCCGGGTCGCGGGTCAGAACCATACGACCACGATAACTGGGCGGCAGATAGAACGGATCTTCCGGGTACAttcgcgattcg                                              >  5:18892/1‑148 (MQ=255)
                                                                                                            agaTACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTcgcgtttcgcgttt                                         <  8:427616/148‑1 (MQ=255)
                                                                                                               tACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGAt                                                                              >  6:40380/1‑108 (MQ=255)
                                                                                                               tACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGAt                                                                              <  5:40380/108‑1 (MQ=255)
                                                                                                               tACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGc                                      >  7:381480/1‑148 (MQ=255)
                                                                                                                  aGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGaa                                   >  8:218456/1‑148 (MQ=255)
                                                                                                                    ccGACCGGGCAGGCTAGCGGGCAGAGGTTACAGGCTTCGCAACGCTGTTCGCCGTCCGGGTCGCGGGTCAGATCGATAGGTCCACTTTAACGGGGCGGCAGATAGACCGGCTCTTCAGGGTACATTCGCGTTTCGCGTTTGGCGAAcg                                 <  5:310988/148‑1 (MQ=255)
                                                                                                                     cGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAAcgc                                >  5:405072/1‑148 (MQ=255)
                                                                                                                        ccGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTg                             >  5:180905/1‑148 (MQ=255)
                                                                                                                         cGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGc                            <  6:202046/148‑1 (MQ=255)
                                                                                                                         cGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGc                            <  6:180905/148‑1 (MQ=255)
                                                                                                                         cGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGc                            <  8:381480/148‑1 (MQ=255)
                                                                                                                               ggCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCg                      <  7:291947/148‑1 (MQ=255)
                                                                                                                               ggCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCg                      <  1:128612/148‑1 (MQ=255)
                                                                                                                                 cTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGat                    <  7:218456/148‑1 (MQ=255)
                                                                                                                                   aCCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACAttcgcgtttcg                                              >  4:22130/1‑120 (MQ=255)
                                                                                                                                   aCCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACAttcgcgtttcg                                              <  3:22130/120‑1 (MQ=255)
                                                                                                                                             ggTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTg                             <  5:434830/127‑1 (MQ=255)
                                                                                                                                             ggTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTg                             >  6:434830/1‑127 (MQ=255)
                                                                                                                                              gTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGAtacta       <  7:378216/148‑1 (MQ=255)
                                                                                                                                               ttACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGAtactac      >  5:92118/1‑148 (MQ=255)
                                                                                                                                                tACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTg                             >  2:174033/1‑124 (MQ=255)
                                                                                                                                                tACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTg                             <  1:174033/124‑1 (MQ=255)
                                                                                                                                                   aGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAAc  <  6:399424/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAAC  >  NC_000913/2396117‑2396411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: