Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,335,762 IS5 (–) +4 bp intergenic (+304/‑66) yciX → / → acnA uncharacterized protein/aconitate hydratase 1; aconitase A

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1335762 =0 (0.000)18 (0.490) 18/256 1.3 100% intergenic (+304/‑69) yciX/acnA uncharacterized protein/aconitate hydratase 1; aconitase A
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 13357650 (0.000)50 (1.350) 39/256 0.1 100% intergenic (+307/‑66) yciX/acnA uncharacterized protein/aconitate hydratase 1; aconitase A
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region

CTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGC  >  NC_000913/1335618‑1335902
                                                                                                                                                |                                                                                                                                            
ctctGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGg                                                                                                                                          >  7:31355‑M1/1‑148 (MQ=255)
acaagaaaatctcttttctggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaccccaggtctagggctccagatgaccaacatgatctcataacaggcacttcttcgcaccgtccTTtgt                                                                                                                                          <  8:125121‑M1/5‑1 (MQ=255)
        atctcttttctggtctgacggcgcttactgctgaattcactgtcggcgacggtaagttgatgtctcatgatgaaccctgtgctatggctccagatgacaaacatgatctcatatccgggacttgttcgccccttccTTTGTGTTATCtt                                                                                                                                  >  7:125121‑M1/137‑149 (MQ=255)
            tcttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaa                                                                                                                               >  7:21747‑M1/1‑136 (MQ=255)
              ttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaat                                                                                                                              <  8:21747‑M1/147‑14 (MQ=255)
                    gtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctcctgatgacaaacatgatctcatatcagggacttgttcgcaccttccTTTGTGTTATCTTTAATATTCAccc                                                                                                                      <  2:110044‑M1/25‑1 (MQ=255)
                    gCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggg                                                                                                                       >  4:142861‑M1/1‑128 (MQ=255)
                     ccGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcgggga                                                                                                                     <  8:31355‑M1/149‑23 (MQ=255)
                               ctGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggt                                                                                                                                    >  5:58918‑M1/1‑117 (MQ=255)
                               ctGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggt                                                                                                                                    <  6:58918‑M1/124‑8 (MQ=255)
                                  actgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctcctgatgacaaacatgatctcatatcagggacttgttcgcaccttccTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAg                                                                                                         >  1:110044‑M1/111‑148 (MQ=255)
                                   aTCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggct                                                                                                       <  1:184524‑M1/149‑37 (MQ=255)
                                        gaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCg                                                                                                  <  2:197309‑M1/45‑1 (MQ=255)
                                                   ccTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcat                                                                                       >  1:40245‑M1/1‑97 (MQ=255)
                                                    cTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatt                                                                                      >  5:93186‑M1/1‑96 (MQ=255)
                                                       gACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcattt                                                                                     <  6:93186‑M1/147‑55 (MQ=255)
                                                        aCCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcat                                                                                       <  2:128988‑M1/144‑53 (MQ=255)
                                                        aCCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcat                                                                                       >  1:128988‑M1/1‑92 (MQ=255)
                                                          cccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttca                                                                                >  3:17357‑M1/1‑90 (MQ=255)
                                                          cccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcg                                                                                                                         >  8:12215‑M1/1‑90 (MQ=255)
                                                          cccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcg                                                                                                                         <  7:12215‑M1/108‑19 (MQ=255)
                                                          cccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCATTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttca                                                                                >  4:129783‑M1/1‑90 (MQ=255)
                                                            cGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatg                                                                              >  3:111682‑M1/1‑88 (MQ=255)
                                                                   atgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATg                                                                       <  5:148974‑M1/72‑1 (MQ=255)
                                                                             atgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTc                                                                                                   >  7:8586‑M1/68‑111 (MQ=255)
                                                                             atgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTc                                                                                                   <  8:8586‑M1/44‑1 (MQ=255)
                                                                              tcttgctttgccaCAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggttctaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttc                                                             <  1:178904‑M1/146‑79 (MQ=255)
                                                                               gaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTa                                                           <  6:157884‑M1/84‑1 (MQ=255)
                                                                                  gctttgcCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctccc                                                         <  3:142861‑M1/148‑83 (MQ=255)
                                                                                  gctttgcAACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttt                                                             >  2:178904‑M1/1‑66 (MQ=255)
                                                                                    tttgcCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgt                                                       >  1:108320‑M1/1‑64 (MQ=255)
                                                                                       gcCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaa                                                    <  4:111682‑M1/148‑88 (MQ=255)
                                                                                       gcCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgt                                                                             <  1:199441‑M1/123‑63 (MQ=255)
                                                                                       gcCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgt                                                                             >  2:199441‑M1/1‑61 (MQ=255)
                                                                                            aaGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgcctt                                              <  2:40245‑M1/149‑94 (MQ=255)
                                                                                                tttCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaat                                          >  3:153659‑M1/1‑52 (MQ=255)
                                                                                                 ttCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaat                                          >  2:5611‑M1/1‑51 (MQ=255)
                                                                                                  tctcCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatc                                         <  4:17357‑M1/148‑99 (MQ=255)
                                                                                                  cagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGaca                                        >  5:157884‑M1/47‑149 (MQ=255)
                                                                                                      ctctTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcc                                     >  3:21339‑M1/1‑46 (MQ=255)
                                                                                                      ctctTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcc                                     >  7:87716‑M1/1‑46 (MQ=255)
                                                                                                       tctTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagccta                                   >  8:137746‑M1/1‑45 (MQ=255)
                                                                                                         atttaTCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctat                                  <  3:129783‑M1/147‑106 (MQ=255)
                                                                                                              tCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagac                            >  5:83475‑M1/1‑38 (MQ=255)
                                                                                                                gatctcatatcagggacttgttcgcaccttccTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCa                            <  1:85298‑M1/115‑1 (MQ=255)
                                                                                                                 atctcatatcagggacttgttcgcaccttccTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCaaa                          >  1:197309‑M1/32‑148 (MQ=255)
                                                                                                                     gggTTGTTATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttc                      <  2:108320‑M1/148‑118 (MQ=255)
                                                                                                                      atatcagggacttgttcgcaccttccTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAag                                                                                  <  3:26987‑M1/61‑1 (MQ=255)
                                                                                                                      atatcagggacttgttcgcaccttccTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAag                                                                                  >  4:26987‑M1/27‑87 (MQ=255)
                                                                                                                       tatcagggacttgttcgcaccttccTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATaaaa                   >  2:85298‑M1/26‑149 (MQ=255)
                                                                                                                       gttgttATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttga                                            >  6:176816‑M1/1‑29 (MQ=255)
                                                                                                                       gttgttATCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttga                                            <  5:176816‑M1/124‑96 (MQ=255)
                                                                                                                        atcagggacttgttcgcaccttccTTTGTGTTATCTTTAATATTCAc                                                                                                                        <  4:154962‑M1/23‑1 (MQ=255)
                                                                                                                             aTCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatt             >  6:9112‑M1/1‑23 (MQ=255)
                                                                                                                              tCAAATCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatt             >  2:158235‑M1/1‑22 (MQ=255)
                                                                                                                                aaaTCGTTACGCGATGTTTGggaaggtgcgaataagcgtggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaa          <  4:21339‑M1/149‑130 (MQ=255)
                                                                                                                                aaaTCGTTACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaa          <  6:83475‑M1/149‑130 (MQ=255)
                                                                                                                                   gttcgcaccttccTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAATCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCactac        <  4:44297‑M1/135‑1 (MQ=255)
                                                                                                                                      ttACGCGATGTTTGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgt     >  7:73966‑M1/1‑14 (MQ=255)
                                                                                                                                        caccttccTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGc  <  7:91279‑M1/141‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                            
CTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGC  >  NC_000913/1335618‑1335902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.