Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,340	C→T	intergenic (‑257/‑30)	ydiY ← / → pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,340	0	C	T	100.0%	97.4 / NA	28	intergenic (‑257/‑30)	ydiY/pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/15);  total (13/15)

ACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAG  >  NC_000913/1806196‑1806445                                                                                                                                                 |                                                                                                          aCTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGa                                                                                                        <  5:22815/148‑1 (MQ=255) aCTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGAc                                                                                                       <  4:4011/149‑1 (MQ=255)            tATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGcc                                                                                            >  8:112349/1‑149 (MQ=255)             aTTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGcc                                                                                            >  5:88325/1‑148 (MQ=255)                               gAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGaa                                                                                                   <  6:128156/123‑1 (MQ=255)                               gAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGaa                                                                                                   >  5:128156/1‑123 (MQ=255)                                        tgtgGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAatgatg                                                                           <  5:32879/138‑1 (MQ=255)                                        tgtgGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAatgatg                                                                           >  6:32879/1‑138 (MQ=255)                                          tgGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAatga                                                                             >  6:103059/1‑134 (MQ=255)                                          tgGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAatga                                                                             <  5:103059/134‑1 (MQ=255)                                                                  atatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCtata                                                                                        <  4:2155/99‑1 (MQ=255)                                                                     tatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATag                                                                                       >  5:196043/1‑97 (MQ=255)                                                                     tatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATag                                                                                       <  6:196043/97‑1 (MQ=255)                                                                       tttATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATaggagg                                                                                   <  2:97568/99‑1 (MQ=255)                                                                       tttATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATaggagg                                                                                   >  1:97568/1‑99 (MQ=255)                                                                           tCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGAt                                    >  6:87050/1‑142 (MQ=255)                                                                           tCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGAt                                    <  5:87050/142‑1 (MQ=255)                                                                             tGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaac                            >  6:179033/1‑148 (MQ=255)                                                                                  aaTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAc                      <  6:88325/149‑1 (MQ=255)                                                                                   aTTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcc                     <  7:112349/149‑1 (MQ=255)                                                                                                cTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGacac                                                     >  5:222137/1‑104 (MQ=255)                                                                                                cTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGacac                                                     <  6:222137/104‑1 (MQ=255)                                                                                                cTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATc        <  5:179033/149‑1 (MQ=255)                                                                                                      tAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctct                                        <  7:67660/111‑1 (MQ=255)                                                                                                      tAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctct                                        >  8:67660/1‑111 (MQ=255)                                                                                                                      tttCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATc        <  1:220307/127‑1 (MQ=255)                                                                                                                      tttCTTCACTTTCCGCTGATTCGGTGCTAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATc        >  2:220307/1‑127 (MQ=255)                                                                                                                                             gTGCTAGACTGAAATCAGCCTAAAGGAGGAAATGATGGTACGTATCTATACGTTGACCATTCCGCCCTCTCGCGATAGCGCAACAATTACCCCGCAAATTAATCCCGAAg  >  5:126683/1‑110 (MQ=255)                                                                                                                                                 |                                                                                                          ACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAG  >  NC_000913/1806196‑1806445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑