Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 743,396 T→C 5.8% F190F (TTT→TTC ybgI → NIF3 family metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913743,3960TC 5.8% 208.1 / 3.0 69F190F (TTT→TTCybgINIF3 family metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (29/36);  new base C (2/2);  total (31/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

GCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACC  >  NC_000913/743249‑743543
                                                                                                                                                   |                                                                                                                                                   
gCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTtta                                                                                                                                                    >  1:185253/1‑149 (MQ=255)
gCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTtta                                                                                                                                                    >  5:338303/1‑149 (MQ=255)
 cTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTttat                                                                                                                                                   <  1:226434/149‑1 (MQ=255)
 cTGGCTTCCGGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGGGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTttat                                                                                                                                                   <  1:122221/149‑1 (MQ=255)
     cTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACt                                                                                                                                               >  7:230292/1‑149 (MQ=255)
          tGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACt                                                                                                                                               <  1:314334/144‑1 (MQ=255)
          tGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGAGGCGGGTAAAGTTTTATCGATAGCGCCGCGCGTTTTTGCGTGGATGCTTTTATTACTGTCg                                                                                                                                           >  8:166207/1‑148 (MQ=255)
          tGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGAGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGAATAAACGATAGCGCCGCGCGAATAGGCGACGATCCATATATTACt                                                                                                                                               >  2:314334/1‑144 (MQ=255)
             aTTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGt                                                                                                                                       >  3:167761/1‑149 (MQ=255)
                 aaGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCt                                                                                                                                   <  2:185253/149‑1 (MQ=255)
                 aaGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGACTGGTGCACGGGCGGCGGGCAAAGTTTTATCGAAAGAGCCGCGCGTTATGGCGTGGATCCTTTTATTACTTGCGAAGTTTCt                                                                                                                                   >  7:243594/1‑149 (MQ=255)
                       gTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGaa                                                                                                                                >  8:132228/1‑146 (MQ=255)
                        tCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGa                                                                                                                            <  2:162895/149‑1 (MQ=255)
                            ggACCTAAGCCGTTAAGGTCTGGCGATACCGGACCTGAGGAGGACCAGCGCCTCTCCAGCTGCACCGCCGCCGGGCAAAGATTTATCTATAGCGCCGCGCGTATTCGCGTGGATGCAGTTATAACAGGCGAAGTTTCTGAACAGACcat                                                                                                                        >  2:153171/1‑149 (MQ=255)
                              aCGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACcatt                                                                                                                       >  7:379394/1‑148 (MQ=255)
                                gTAAGACGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACcattcat                                                                                                                    <  7:403381/149‑1 (MQ=255)
                                 gaaGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACcattcatt                                                                                                                   <  3:54791/148‑1 (MQ=255)
                                   aGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACcattcatt                                                                                                                   >  8:248240/1‑147 (MQ=255)
                                    gCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAg                                                                                                                <  5:237036/149‑1 (MQ=255)
                                                tgGGGATACCGGTCCTGAGGTGGTCCAGGGGGTCGCCTGGTGGAAGGGAGGCGGGCAAAGTTTTATCGATAGCGCCGCGGGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCGGCGAGCAgt                                                                                                    <  8:243594/149‑2 (MQ=255)
                                                       aCCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCa                                                                                             >  6:328458/1‑149 (MQ=255)
                                                           gACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCAtttt                                                                                         <  8:57911/149‑1 (MQ=255)
                                                             ccTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTa                                                                                       <  5:111803/149‑1 (MQ=255)
                                                              cTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTAt                                                                                      >  7:118106/1‑149 (MQ=255)
                                                              cTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTAt                                                                                      <  6:125916/149‑1 (MQ=255)
                                                               tGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTAtg                                                                                     >  5:146851/1‑149 (MQ=255)
                                                                 aGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTAtgc                                                                                    <  7:32895/148‑1 (MQ=255)
                                                                 aGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTAtg                                                                                     <  3:347352/147‑1 (MQ=255)
                                                                 aGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTAtg                                                                                     <  6:338303/147‑1 (MQ=255)
                                                                 aGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTAtg                                                                                     <  5:328458/147‑1 (MQ=255)
                                                                   gtggtCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTCATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTAtgctg                                                                                  >  6:85275/1‑148 (MQ=255)
                                                                   gtggtCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTCATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTAtgctg                                                                                  >  2:146477/1‑148 (MQ=255)
                                                                    tggtCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGGCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCa                                                                                <  1:271086/149‑1 (MQ=255)
                                                                     ggtCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGTCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAg                                                                               <  8:211799/149‑1 (MQ=255)
                                                                     gggccAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAg                                                                               <  6:64398/146‑1 (MQ=255)
                                                                      gtCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAg                                                                               <  8:230292/148‑1 (MQ=255)
                                                                      gtCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAg                                                                               <  7:132228/148‑1 (MQ=255)
                                                                       tCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGt                                                                             >  5:165139/1‑149 (MQ=255)
                                                                        ccAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTAtgctg                                                                                  <  7:335376/143‑1 (MQ=255)
                                                                        ccAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTAtgctg                                                                                  >  8:335376/1‑143 (MQ=255)
                                                                        ccAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTAGCGATAGCGCCGCGCGTGTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAAAAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTCCAggg                                                                             >  2:316604/1‑147 (MQ=255)
                                                                         cAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCa                                                                           <  1:93528/149‑1 (MQ=255)
                                                                               gTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGc                                                                     <  6:165139/149‑1 (MQ=255)
                                                                                  gCCTGGTGCACGGGCGGCGGGTAAAGTTTTATCTATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCa                                                                   <  7:166207/148‑1 (MQ=255)
                                                                                    cTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACcattcatt                                                                                                                   >  7:138750/1‑98 (MQ=255)
                                                                                    cTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACcattcatt                                                                                                                   <  8:138750/98‑1 (MQ=255)
                                                                                       gTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGTCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGaa                                                              <  3:292598/148‑1 (MQ=255)
                                                                                          cACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTCATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACgt                                                           <  1:146477/148‑1 (MQ=255)
                                                                                          cACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTCATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACgt                                                           <  5:85275/148‑1 (MQ=255)
                                                                                             gggCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGATCAGACGATTGATTCAGCCGGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACgtggtg                                                       >  1:32716/1‑149 (MQ=255)
                                                                                                   cggGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTc                                                 >  4:83501/1‑149 (MQ=255)
                                                                                                    gggCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTcg                                                >  3:96201/1‑149 (MQ=255)
                                                                                                      gCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAAc                                                             >  8:302187/1‑134 (MQ=255)
                                                                                                      gCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAAc                                                             <  7:302187/134‑1 (MQ=255)
                                                                                                       cAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTcgcg                                              >  3:106984/1‑148 (MQ=255)
                                                                                                         aaGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCAt                                           >  4:42536/1‑149 (MQ=255)
                                                                                                           gTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCAtt                                          >  6:185012/1‑148 (MQ=255)
                                                                                                            ttttATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCAtt                                          >  2:75710/1‑147 (MQ=255)
                                                                                                               tATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGc                                      <  4:167761/148‑1 (MQ=255)
                                                                                                                aTCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGc                                      >  5:70879/1‑147 (MQ=255)
                                                                                                                   gATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGt                                  <  6:146851/148‑1 (MQ=255)
                                                                                                                         gccgcGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCtgaa                           <  1:75710/149‑1 (MQ=255)
                                                                                                                             cgcgTTTTGGCGTGGATGCTATTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCAGCAGGTCACCATGCCCCTGAACGAGGTGGTATTCGAGCATTGAGCGAGTGGCtcaatga                        >  7:203406/1‑148 (MQ=255)
                                                                                                                              gcgTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTTCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTTGCTGAATGaaa                      <  1:316604/149‑1 (MQ=255)
                                                                                                                                gtgttGGCGTGGAGGCTTTTATTACTGGCGAAGCTTCTGAACAGACCATTCCTTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCCTGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAAt                    <  2:401678/146‑1 (MQ=255)
                                                                                                                                      gCGTGGATGCTTTTATTACTGGCGAACTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTAAGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGa               <  2:32716/148‑1 (MQ=255)
                                                                                                                                        gTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCt            <  7:248240/149‑1 (MQ=255)
                                                                                                                                         tGGATGCTTTTATTATTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCtt           >  2:337169/1‑149 (MQ=255)
                                                                                                                                         tGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCtt           >  2:97649/1‑149 (MQ=255)
                                                                                                                                           gATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTg          <  6:70879/148‑1 (MQ=255)
                                                                                                                                           gATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGa         >  6:268235/1‑149 (MQ=255)
                                                                                                                                            aTGCTTTTATTACTGGCGCAGTTTCTGAACAGACCATTCATTCCGCCCGCGAGCAGGGATTGCATTTTTAAGCTGCAGGTCACCCTGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGAt        <  2:191822/149‑1 (MQ=255)
                                                                                                                                            aTGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGAt        <  5:185012/149‑1 (MQ=255)
                                                                                                                                                  ttattaCTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGAcc  >  6:342083/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACCGGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGATAGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATTCATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACC  >  NC_000913/743249‑743543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: