Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,235,689 C→T 9.2% intergenic (+32/+20) fadR → / ← ycgB fatty acid metabolism regulon transcriptional regulator/SpoVR family stationary phase protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,235,6890CT 9.2% 225.3 / 9.1 76intergenic (+32/+20)fadR/ycgBfatty acid metabolism regulon transcriptional regulator/SpoVR family stationary phase protein
Reads supporting (aligned to +/- strand):  ref base C (38/31);  new base T (3/4);  total (41/35)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01

AAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCC  >  NC_000913/1235542‑1235835
                                                                                                                                                   |                                                                                                                                                   
aaGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAAc                                                                                                                                                     >  3:231181/1‑148 (MQ=255)
 aGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGTTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGa                                                                                                                                                   <  2:183115/149‑1 (MQ=255)
 aGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCGCTTCCGTTTAAAGAGCAAACCCCTCAAAc                                                                                                                                                     >  1:179488/1‑147 (MQ=255)
 aGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCTAACCCCTCAAACGa                                                                                                                                                   <  8:116766/149‑1 (MQ=255)
 aGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGa                                                                                                                                                   <  2:172302/149‑1 (MQ=255)
       gcACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCGTTTGAGGGGtt                                                                                                                                            <  3:191729/149‑1 (MQ=255)
         aCGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGtttt                                                                                                                                          >  5:308386/1‑149 (MQ=255)
          cGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGttttt                                                                                                                                         <  7:138013/149‑1 (MQ=255)
            aTCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCGTTTGAGGGGtttgctc                                                                                                                                       <  3:368773/149‑5 (MQ=255)
             tCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTttgt                                                                                                                                      >  5:89358/1‑149 (MQ=255)
                     aagAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTa                                                                                                                              >  2:9618/3‑149 (MQ=255)
                      cGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTa                                                                                                                              >  5:149381/1‑148 (MQ=255)
                      cGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTa                                                                                                                              <  7:183490/148‑1 (MQ=255)
                       gAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTa                                                                                                                              >  6:27120/1‑147 (MQ=255)
                         aaCAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGtttt                                                                                                                                          >  2:4572/1‑133 (MQ=255)
                         aaCAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGtttt                                                                                                                                          <  1:4572/133‑1 (MQ=255)
                              tGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTcc                                                                                                                     >  8:274761/1‑149 (MQ=255)
                                   cGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTc                                                                                                                >  5:290382/1‑149 (MQ=255)
                                     cTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCtt                                                                                                              >  4:333253/1‑149 (MQ=255)
                                        tGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCtt                                                                                                              >  5:266536/1‑146 (MQ=255)
                                        tGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCtt                                                                                                              <  6:266536/146‑1 (MQ=255)
                                        tGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGc                                                                                                           >  3:188229/1‑149 (MQ=255)
                                          ggCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCgg                                                                                                         <  7:274761/149‑1 (MQ=255)
                                           gCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGc                                                                                                           <  6:399332/146‑1 (MQ=255)
                                           gCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGc                                                                                                           >  5:399332/1‑146 (MQ=255)
                                           gCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCggg                                                                                                        <  1:9618/149‑1 (MQ=255)
                                           gCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCggg                                                                                                        >  2:352868/1‑149 (MQ=255)
                                                    tGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTcc                                                                                               <  6:308386/149‑1 (MQ=255)
                                                      gCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGGGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAg                                                                                             <  2:24327/149‑1 (MQ=255)
                                                       cGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAg                                                                                             >  7:356730/1‑148 (MQ=255)
                                                           aTTTGGCAGCGGATGCAGAAATATCTGCCGGTTGATTTAGCGATTCAGGGGCGATAATCCCTTCAGTTTAAAGAGCTAACCCCTCAAACGAGTGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAAc                                                                                         <  8:92913/148‑1 (MQ=255)
                                                            tttGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTc                                                                                       >  1:301046/1‑149 (MQ=255)
                                                               ggCACCGTATGCAGAAAAATCTGCCGGGTTATTTAGCCATTCAGGGGCTATAATCCCTTCCGTTTAAACCGCAAACCCCACAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGa                                                                                     <  2:179488/148‑1 (MQ=255)
                                                                gCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGAt                                                                                    <  7:179254/148‑1 (MQ=255)
                                                                  aCCGGATGCAGAAAAAACAGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTGTTTTGTTGATTATACAGCGTTCCCATTCTTGGCGGGCAACGTTCCAGCACCTCGAtgcg                                                                                 >  6:304061/1‑148 (MQ=255)
                                                                           aGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTTCCGTCTTc                                                                        >  7:83410/1‑149 (MQ=255)
                                                                           aGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTc                                                                        >  7:238055/1‑149 (MQ=255)
                                                                           aGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTc                                                                        <  5:210338/149‑1 (MQ=255)
                                                                            gAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCg                                                                       >  2:19473/1‑149 (MQ=255)
                                                                              aaaaTCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGtt                                                                     <  6:149381/149‑1 (MQ=255)
                                                                                  tCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGc                                                                 >  3:330892/1‑149 (MQ=255)
                                                                                    tGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTg                                                               >  3:255306/1‑149 (MQ=255)
                                                                                      ccGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTc                                                                                                <  5:63018/114‑1 (MQ=255)
                                                                                      ccGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTc                                                                                                >  6:63018/1‑114 (MQ=255)
                                                                                         ggTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGa                                                          <  4:255306/149‑1 (MQ=255)
                                                                                             aTTTAGCCATTCAGGGGCGATAATCACTTCCGTTTAAAGAGCAAACCCCTCAAACGAGCGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGcat                                                      >  6:375496/1‑149 (MQ=255)
                                                                                               ttAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTg                                                                  >  5:46550/1‑135 (MQ=255)
                                                                                               ttAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTg                                                                  <  6:46550/135‑1 (MQ=255)
                                                                                                     aTTCATGGTCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGcc                                                                              <  8:294301/117‑1 (MQ=255)
                                                                                                     aTTCAGGGGCGATAATCCCTTCCGTTTAAAGATCAAACCCCTCAAACGATGGGTTTTTTGGTGTTTTTACAGATTTCCCAGTCTTGGCGGGCAACGTTCCAGCAACTAGATGCTGcc                                                                              >  7:294301/1‑117 (MQ=255)
                                                                                                        cAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATc                                           >  6:1246/1‑149 (MQ=255)
                                                                                                         aGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTAACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATcc                                          >  6:300891/1‑149 (MQ=255)
                                                                                                          ggggCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATccc                                         <  8:356730/149‑1 (MQ=255)
                                                                                                             gCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCcac                                      <  4:231181/149‑1 (MQ=255)
                                                                                                                aTAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAgg                                   >  6:380282/1‑149 (MQ=255)
                                                                                                                    tCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGcatcacat                                                 >  7:65541/1‑131 (MQ=255)
                                                                                                                    tCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGcatcacat                                                 <  8:65541/131‑1 (MQ=255)
                                                                                                                     cccTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGa                                <  2:205757/147‑1 (MQ=255)
                                                                                                                     cccTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGa                                >  1:205757/1‑147 (MQ=255)
                                                                                                                      ccTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATg                              <  5:380282/148‑1 (MQ=255)
                                                                                                                          ccGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGcac                           <  4:188229/147‑1 (MQ=255)
                                                                                                                                 aaGAGCAATCCCCTCAAACGAGGGGTTTTTTGTTGTTGTTACAGAATTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATACCCACAGGCGATGCACATGCTTCAg                  <  6:290382/149‑1 (MQ=255)
                                                                                                                                   gagCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCACCTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGa                >  5:151656/1‑149 (MQ=255)
                                                                                                                                   gagCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGa                >  5:157687/1‑149 (MQ=255)
                                                                                                                                   gagCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTATTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGa                >  7:182489/1‑149 (MQ=255)
                                                                                                                                    aaaaaaCCCCTCGTTTGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGcatcacatc                                                <  6:128837/113‑1 (MQ=255)
                                                                                                                                    aaaaaaCCCCTCGTTTGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGcatcacatc                                                >  5:128837/4‑116 (MQ=255)
                                                                                                                                      aaaaCCCCTCGTTTGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGc                                                                 >  6:399745/2‑97 (MQ=255)
                                                                                                                                      aaaaCCCCTCGTTTGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGc                                                                 <  5:399745/96‑1 (MQ=255)
                                                                                                                                      aaaaCCCCTCGTTTGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGAct              >  2:42975/2‑148 (MQ=255)
                                                                                                                                       aaaCCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCACCTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGActtc            <  1:83666/149‑1 (MQ=255)
                                                                                                                                        aaCCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGActtct           >  8:227048/1‑149 (MQ=255)
                                                                                                                                         aCCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGActtctt          >  3:108443/1‑149 (MQ=255)
                                                                                                                                          ccccTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCttt         <  8:377397/149‑1 (MQ=255)
                                                                                                                                           cccTCAAACGAGGGGTTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCttt         >  4:383601/1‑149 (MQ=255)
                                                                                                                                              tCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTgcgc     >  6:156186/1‑149 (MQ=255)
                                                                                                                                               cAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGcc    >  5:389178/1‑149 (MQ=255)
                                                                                                                                                 aaCGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGcccc  <  4:330892/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCC  >  NC_000913/1235542‑1235835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: