Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,736,692 C→T 5.5% R200H (CGT→CAT)  ydhP ← putative MFS transporter, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,736,6920CT 5.5% 237.0 / 2.1 73R200H (CGT→CAT) ydhPputative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (32/37);  new base T (2/2);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

ACACCAATCAGCACCAGCATTGCCGTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAAC  >  NC_000913/1736548‑1736838
                                                                                                                                                |                                                                                                                                                  
acacCAATCAGCACCAGCATTGCCGTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCAt                                                                                                                                                <  1:107222/149‑1 (MQ=255)
   ccAATCAGCACCAGCATTGCCGTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTaa                                                                                                                                             <  2:275679/149‑1 (MQ=255)
    cAATCAGCACCAGCATTGCCGTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTaa                                                                                                                                             >  8:347769/1‑148 (MQ=255)
     aaTCAGCACCAGCATTGCCGTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATa                                                                                                                                           <  8:78373/149‑1 (MQ=255)
       tCAGCACCAGCATTGCCGTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATAcc                                                                                                                                         <  7:262911/149‑1 (MQ=255)
         aGCCCCAGCATTGCCGTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGGGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCCGCAATGCAGACAGCACCTGCGGACGCATTAATACCGc                                                                                                                                       <  7:306206/149‑1 (MQ=255)
            aCCAGCATTGCCGTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAg                                                                                                                                    >  6:356098/1‑149 (MQ=255)
                gCATTGCCGTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGc                                                                                                                                       >  5:180855/1‑142 (MQ=255)
                gCATTGCCGTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGc                                                                                                                                       <  6:180855/142‑1 (MQ=255)
                   ttGCCGTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCtttt                                                                                                                             >  1:336065/1‑149 (MQ=255)
                   ttGCCGTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCtttt                                                                                                                             >  5:381854/1‑149 (MQ=255)
                     gCCGTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCttttt                                                                                                                            <  4:223083/148‑1 (MQ=255)
                        gTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCAc                                                                                                                        <  8:34934/149‑1 (MQ=255)
                        gTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCAc                                                                                                                        <  4:342360/149‑1 (MQ=255)
                         tGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTaa                                                                                                                                             >  2:98916/1‑127 (MQ=255)
                         tGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTaa                                                                                                                                             <  1:98916/127‑1 (MQ=255)
                           aCGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACtt                                                                                                                      >  8:155647/1‑148 (MQ=255)
                           aCGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTc                                                                                                                     <  3:187844/149‑1 (MQ=255)
                           aCGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTc                                                                                                                     <  6:340575/149‑1 (MQ=255)
                            cGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCa                                                                                                                    <  8:307386/149‑1 (MQ=255)
                                cacCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTc                                                                                                                >  8:169987/1‑149 (MQ=255)
                                  ccGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGt                                                                                                              <  7:402631/149‑1 (MQ=255)
                                   cGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTg                                                                                                             <  4:205008/149‑1 (MQ=255)
                                      tgtTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGccc                                                                                                          >  7:62722/1‑149 (MQ=255)
                                          gCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTg                                                                                                       <  4:393793/148‑1 (MQ=255)
                                          gCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTg                                                                                                       >  3:393793/1‑148 (MQ=255)
                                           cGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGc                                                                                                      <  5:149653/148‑1 (MQ=255)
                                                gTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCaccacc                                                                                                >  5:223838/1‑149 (MQ=255)
                                                 tAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGAGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCt                                                                                               <  6:161640/149‑1 (MQ=255)
                                                  aaTACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCtt                                                                                              >  7:173502/1‑149 (MQ=255)
                                                  aaTACTATGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACAACCTCCCAGTACAGTCGTCAGCAATGCAGACAGCACCTGCGGATGCATGAATCCCCCCATCTCTTTTTACACTTCAGGACCAGCCGCAGcaccacact                                                                                              >  1:137738/1‑146 (MQ=255)
                                                     aCTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAg                                                                                           >  1:22478/1‑149 (MQ=255)
                                                      cTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAg                                                                                           <  8:409228/148‑1 (MQ=255)
                                                      cTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAg                                                                                           <  5:356098/148‑1 (MQ=255)
                                                         tGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTa                                                                                        <  7:169987/148‑1 (MQ=255)
                                                         tGCAGGACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTaa                                                                                       >  1:274279/1‑149 (MQ=255)
                                                                ccGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGagaa                                                                                 <  8:71269/148‑1 (MQ=255)
                                                                ccGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGagaa                                                                                 <  6:381854/148‑1 (MQ=255)
                                                                 cGGAGAGCTATAGGTGTAGAGAGTAAACGGTGCAGCAGCTCCCAGTACCGTCGTCAGAAATGCAGACAGCACCTGCGGCCGCATTACTCCCGCCAGCTCTTTTTTGACTTCAGGTCGTGCCGCTGCACCAGCTGGAGGTAATGagaag                                                                                >  8:397797/1‑148 (MQ=255)
                                                                 cGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGATGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGagaag                                                                                >  3:77219/1‑148 (MQ=255)
                                                                      agaTATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGa                                                                          <  2:164593/149‑1 (MQ=255)
                                                                        atatAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACt                                                                        <  3:128586/149‑1 (MQ=255)
                                                                          ataGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCGTTAGGGAATGAGAAGCACAGCCTTa                                                                      >  6:235969/1‑149 (MQ=255)
                                                                               tgtAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGCCAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCAtt                                                                 >  8:270835/1‑149 (MQ=255)
                                                                                 tAGAGAGTAATCATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTg                                                                >  6:204944/1‑148 (MQ=255)
                                                                                    agagTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATTCAGACAGCCCCGGCGGACGCATTAATACCGCCAGCTCTTTTTTCCCTTCAGGTCGTGCCCCTGCACCCCCGTTAGGTAATGAGAAGCACAGACTTACCAGTGACAt                                                            >  6:220130/1‑149 (MQ=255)
                                                                                        tAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACt                                                        >  6:210389/1‑149 (MQ=255)
                                                                                         aaaCATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGa                                                                          >  4:240744/1‑130 (MQ=255)
                                                                                         aaaCATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGa                                                                          <  3:240744/130‑1 (MQ=255)
                                                                                          aaCATTGCACCAGCTCCCAGTACCGTCGTCAGCACTGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTcc                                                      <  2:382425/149‑1 (MQ=255)
                                                                                           aCATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTACCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTcc                                                      >  3:49848/1‑148 (MQ=255)
                                                                                                 cACCAGGTTCCTTTACCGTCGTCTGCAATGCAGACAGCACCTGCGGACGCATTAATACCGGCAGTTCTTTTTTCACTTCAGGTCGTGCCACTGCACCACCTTTTGGTATTGAGAAGATCAGAGTTATCATTGAAATCACTCGCAGcccc                                               <  4:262858/149‑1 (MQ=255)
                                                                                                    cAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCttt                                                                                                                              >  6:265137/1‑67 (MQ=255)
                                                                                                    cAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCttt                                                                                                                              >  2:369776/1‑67 (MQ=255)
                                                                                                    cAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCttt                                                                                                                              <  5:265137/67‑1 (MQ=255)
                                                                                                    cAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCttt                                                                                                                              <  1:369776/67‑1 (MQ=255)
                                                                                                      gCTCCCAGTACCGTCGGCAGCAATGCAGACAGCACCTGCTGACGCATTAATACCGCCATCTCGTTTTTCACTTCCGGGCGTCCCCCTTCACCACCTTTAGGGAATGAGAAGAACAGACTTACCATTGAACTCACTCCCAGCCCCGCCGt                                          <  4:83516/149‑1 (MQ=255)
                                                                                                       cTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGtt                                         <  7:347769/149‑1 (MQ=255)
                                                                                                       cTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGtt                                         <  8:173502/149‑1 (MQ=255)
                                                                                                       cTCCCAGTACCGGCGTCTGCAAGTAAGACAGCACCTGCGGATGCATTAATACGGCCAGCTCTTTTTTCACTTCTGGTCGTGCCCCTGCTCCACCTTTAGGTTATGAGATGAACAGTCTTTCCATTGAAATCACTCCGAGCCCCGCCGtt                                         <  2:137738/149‑1 (MQ=255)
                                                                                                                   tcgtcAGCAATGCAGACAGCACCTGCGGATGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGAc                             <  4:77219/149‑1 (MQ=255)
                                                                                                                     gtcAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGt                                          >  7:113183/1‑134 (MQ=255)
                                                                                                                     gtcAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGt                                          <  8:113183/134‑1 (MQ=255)
                                                                                                                      tcAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATc                          <  7:270835/149‑1 (MQ=255)
                                                                                                                      tcAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATc                          >  6:288779/1‑149 (MQ=255)
                                                                                                                      tcAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATc                          >  1:292996/1‑149 (MQ=255)
                                                                                                                      tcAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATc                          <  6:223838/149‑1 (MQ=255)
                                                                                                                         gCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGc                       >  4:301229/1‑149 (MQ=255)
                                                                                                                          cAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGc                       >  3:45717/1‑148 (MQ=255)
                                                                                                                                agccAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATAACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGa                >  4:242884/4‑149 (MQ=255)
                                                                                                                                aGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTACCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGa                <  4:49848/149‑1 (MQ=255)
                                                                                                                                  aCAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATg              >  1:212844/1‑149 (MQ=255)
                                                                                                                                               aCGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAAAAGACTTACCATTGAAATCACTCACATCCCCGCCGTTGCCAGAAATGACATCCGCCAGACGATGTTGTCACCCAAc  >  5:331171/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
ACACCAATCAGCACCAGCATTGCCGTGACGAACACCGGTGTTGCGTGGGTAATACTTTGCAGTACCGGAGAGATATAGGTGTAGAGAGTAAACATTGCACCAGCTCCCAGTACCGTCGTCAGCAATGCAGACAGCACCTGCGGACGCATTAATACCGCCAGCTCTTTTTTCACTTCAGGTCGTGCCCCTGCACCACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAAC  >  NC_000913/1736548‑1736838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: