Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,806,974	C→T	18.0%	P202L (CCG→CTG)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,974	0	C	T	18.0%	204.0 / 35.2	89	P202L (CCG→CTG)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (33/40);  new base T (8/8);  total (41/48)
Fisher's exact test for biased strand distribution p-value = 7.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-02

TTTCCGCTGCGCAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCA  >  NC_000913/1806827‑1807115                                                                                                                                                    |                                                                                                                                              tttCCGCTGCGCAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGACCTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCg                                                                                                                                              <  5:194088/149‑1 (MQ=255) tttCCGCTGCGCAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAAGTCACCCAGCCg                                                                                                                                              >  6:386378/1‑149 (MQ=255)       cTGCGCAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGAt                                                                                                                                        >  1:41476/1‑149 (MQ=255)          cgcAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCGGTGCGCTGGTGAATCGCGAACTCACCCAGCTGGACGATGTc                                                                                                                                     <  1:397373/149‑1 (MQ=255)           gcAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCTGGACGATGTcc                                                                                                                                    <  3:229912/149‑1 (MQ=255)           gcAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCTGGACGATGTcc                                                                                                                                    >  2:75301/1‑149 (MQ=255)           gcAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTcc                                                                                                                                    <  6:73522/149‑1 (MQ=255)             aaaaaCAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGc                                                                                                                                  <  6:67654/149‑1 (MQ=255)                aaCAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCaaa                                                                                                                               <  3:245602/149‑1 (MQ=255)                aaCAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCaaa                                                                                                                               <  5:324652/149‑1 (MQ=255)                     gggATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCcgc                                                                                                                          >  4:166054/1‑149 (MQ=255)                        aTCCGATGCATCGTCGACAGGTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCa                                                                                                                       <  8:228029/149‑1 (MQ=255)                          ccGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAg                                                                                                                      >  3:289729/1‑148 (MQ=255)                             cTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGaaa                                                                                                                  <  4:289729/149‑1 (MQ=255)                                  tcgtcgACAGTTCTGGCGAAGCGTTAAGGGCAGCACTGGCAATTGGCAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATAGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGtt                                                                                                             <  8:101900/149‑1 (MQ=255)                                    gtcgACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAAGTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTa                                                                                                            <  5:386378/148‑1 (MQ=255)                                     tcgACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTaa                                                                                                           >  3:377295/1‑148 (MQ=255)                                       gACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCTGGACGATGTCCGCAAAGCCGCGCAGGAAATCGtacagtt                                                                                                        >  5:403201/1‑143 (MQ=255)                                          aGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCgg                                                                                                     >  7:15459/1‑149 (MQ=255)                                                 gCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAAGTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAg                                                                                                 <  2:41476/146‑1 (MQ=255)                                                 gCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCTGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGc                                                                                               >  1:264748/1‑148 (MQ=255)                                                 gCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGAc                                                                                                                                           <  3:380332/104‑1 (MQ=255)                                                 gCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGAc                                                                                                                                           >  4:380332/1‑104 (MQ=255)                                                   gAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCTGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCa                                                                                             >  7:10471/1‑148 (MQ=255)                                                    aaGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATa                                                                                                         >  2:271011/1‑135 (MQ=255)                                                    aaGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATa                                                                                                         <  1:271011/135‑1 (MQ=255)                                                    aaGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCaaa                                                                                           <  4:78440/149‑1 (MQ=255)                                                      gCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGGGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACg                                                                                         <  8:347103/149‑1 (MQ=255)                                                        gTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCTGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACggg                                                                                       <  2:264748/149‑1 (MQ=255)                                                           aaGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCTGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGtt                                                                                     <  8:10471/148‑1 (MQ=255)                                                            aGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGt                                                                                   <  2:201329/149‑1 (MQ=255)                                                             gTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAAGTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTc                                                                                  >  5:371488/1‑149 (MQ=255)                                                             gTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTc                                                                                  >  7:46119/1‑149 (MQ=255)                                                                 agcaCTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGttt                                                                              >  5:116390/1‑149 (MQ=255)                                                                  gcaCTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCTGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTc                                                                             <  1:75301/149‑1 (MQ=255)                                                                     cTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCTCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCt                                                                          <  2:404966/149‑1 (MQ=255)                                                                     cTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCt                                                                          <  5:257430/149‑1 (MQ=255)                                                                     cTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCt                                                                          <  4:377295/149‑1 (MQ=255)                                                                        gCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCTGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTggg                                                                       >  1:160864/1‑149 (MQ=255)                                                                          aaTTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTc                                                                     <  3:166054/149‑1 (MQ=255)                                                                           aTTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCTGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTg                                                                         >  2:275530/1‑144 (MQ=255)                                                                           aTTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCTGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTg                                                                         <  1:275530/144‑1 (MQ=255)                                                                            ttGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCca                                                                   >  5:287626/1‑149 (MQ=255)                                                                                tAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAg                                                               >  3:319285/1‑149 (MQ=255)                                                                                tAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAg                                                               <  8:21417/149‑1 (MQ=255)                                                                                  aCATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGt                                                                      <  5:258159/140‑1 (MQ=255)                                                                                  aCATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGt                                                                      >  6:258159/1‑140 (MQ=255)                                                                                          ttGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTggg                                                     >  7:204704/1‑149 (MQ=255)                                                                                          ttGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTggg                                                     >  8:65553/1‑149 (MQ=255)                                                                                                  gCCTAACCAAAAAGTACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATa                                             <  3:271806/149‑1 (MQ=255)                                                                                                  gCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAAGTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGa                                               >  7:227062/1‑147 (MQ=255)                                                                                                  gCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGTCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGa                                               >  2:31818/1‑147 (MQ=255)                                                                                                  gCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATa                                             <  5:88134/149‑1 (MQ=255)                                                                                                    cTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGGCCACAAGGAGCGCTGGGTGTTGATAGt                                           <  1:16778/149‑1 (MQ=255)                                                                                                     tAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAgg                                                              <  8:202684/129‑1 (MQ=255)                                                                                                     tAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAgg                                                              >  7:202684/1‑129 (MQ=255)                                                                                                        ccAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaa                                        >  4:183049/1‑148 (MQ=255)                                                                                                         cAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaaaa                                      >  5:356753/1‑149 (MQ=255)                                                                                                         cAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaaaa                                      <  6:356753/149‑1 (MQ=255)                                                                                                          aaaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTc                                                                                  <  7:343139/104‑1 (MQ=255)                                                                                                          aaaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTc                                                                                  >  8:343139/1‑104 (MQ=255)                                                                                                          aaaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaaaa                                      >  8:168754/1‑148 (MQ=255)                                                                                                          aaaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAAc                                     >  6:226525/1‑149 (MQ=255)                                                                                                            aaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATa                                             <  2:397868/139‑1 (MQ=255)                                                                                                            aaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTg                                   <  8:46119/149‑1 (MQ=255)                                                                                                            aaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCACTGGGTCCACAATGAGCGCTGGGTGTTGATa                                             >  1:397868/1‑139 (MQ=255)                                                                                                               gAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGTCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTAt                                <  1:31818/149‑1 (MQ=255)                                                                                                                     aGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAg                            <  3:183049/147‑1 (MQ=255)                                                                                                                                 aaTCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCcaccac               <  6:116390/148‑1 (MQ=255)                                                                                                                                   tCGCGAACTCACCCAGCTGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCa                             <  6:321760/132‑1 (MQ=255)                                                                                                                                   tCGCGAACTCACCCAGCTGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCa                             >  5:321760/1‑132 (MQ=255)                                                                                                                                   tCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACggg                                                                                       >  1:143158/1‑74 (MQ=255)                                                                                                                                   tCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACggg                                                                                       <  2:143158/74‑1 (MQ=255)                                                                                                                                   tCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAAAGGGTTGTCGTTTCCCTGGTTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAgg            >  6:230823/1‑149 (MQ=255)                                                                                                                                   tCGCGAACTCACCCACCCGGACGATGTCCGCAAAGCCTCGCAGGAAAACGTTAATAGCGGCAAGCCCAAACGGGTTGTCGTTTCCCTGGGTCCACAACGAGCGCTGTGTCATGATAGTGAAAACTGTATTCAGTTGGTGCCACCACAgg            >  4:150279/1‑149 (MQ=255)                                                                                                                                     gcgAACTCACCCAGCTGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAg                            >  7:163183/1‑131 (MQ=255)                                                                                                                                     gcgAACTCACCCAGCTGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAg                            <  8:163183/131‑1 (MQ=255)                                                                                                                                     gcgAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGt           <  7:168754/148‑1 (MQ=255)                                                                                                                                         aCTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGaaaa      <  3:150279/149‑1 (MQ=255)                                                                                                                                         aCTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGaaaa      <  4:319285/149‑1 (MQ=255)                                                                                                                                          gtCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAg     >  8:79365/2‑149 (MQ=255)                                                                                                                                          cTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGGGGCAAGGAGAATCTGTTTGTCTTTTGCCTGGGTCCTCAAGGAGCTCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAg     <  8:15459/149‑1 (MQ=255)                                                                                                                                           tCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaaaa                                      >  1:355930/1‑115 (MQ=255)                                                                                                                                           tCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaaaa                                      <  2:355930/115‑1 (MQ=255)                                                                                                                                             aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCa  <  7:79365/149‑1 (MQ=255)                                                                                                                                             aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGc    >  4:226371/1‑147 (MQ=255)                                                                                                                                               ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGcc   <  6:330712/146‑1 (MQ=255)                                                                                                                                               ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGcc   >  5:330712/1‑146 (MQ=255)                                                                                                                                               ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACAGGTGAAAAGCCa  >  6:385317/1‑147 (MQ=255)                                                                                                                                                    |                                                                                                                                              TTTCCGCTGCGCAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCA  >  NC_000913/1806827‑1807115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑