Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,374,901 A→T 5.7% L11F (TTA→TTT dcuD → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,374,9010AT 5.7% 359.4 / 8.1 117L11F (TTA→TTTdcuDputative transporter
Reads supporting (aligned to +/- strand):  ref base A (59/51);  new base T (3/4);  total (62/55)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 134 total aligned reads displayed.

ACAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATA  >  NC_000913/3374755‑3375049
                                                                                                                                                   |                                                                                                                                                    
aCAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGTGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTattaat                                                                                                                                                    >  5:125712/1‑149 (MQ=255)
aCAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTattaat                                                                                                                                                    >  7:406860/1‑149 (MQ=255)
   aTTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATAAAACATCGTTGTTATCAATCTGCCGTTTATGGGCTTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTAc                                                                                                                                                 <  2:263285/149‑1 (MQ=255)
    ttAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGtaattaatac                                                                                                                                                  >  4:232668/1‑146 (MQ=255)
    ttAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTTATTAc                                                                                                                                                 >  4:40644/1‑148 (MQ=255)
    ttAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTAc                                                                                                                                                 >  7:127790/1‑148 (MQ=255)
       aaaaCACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATg                                                                                                                                             <  8:392736/149‑1 (MQ=255)
       aaaaCACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAAACTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATg                                                                                                                                             >  5:84520/1‑149 (MQ=255)
         aaCACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATggg                                                                                                                                           <  2:414946/149‑1 (MQ=255)
                  tCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGAt                                                                                                                                  >  8:62770/1‑149 (MQ=255)
                   ccGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATc                                                                                                                                 >  4:102296/1‑149 (MQ=255)
                             aaTAGCCTTTCAAAAAATATAACATAGTTGTTTTCAATCTGCCGTTTAGGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACt                                                                                                                       <  6:373547/149‑1 (MQ=255)
                                    ttGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAAcc                                                                                                                >  1:313720/1‑149 (MQ=255)
                                    ttGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACAGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAAcc                                                                                                                >  6:289103/1‑149 (MQ=255)
                                     tGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCt                                                                                                               >  8:268583/1‑149 (MQ=255)
                                        aaaaaaTATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAg                                                                                                            <  1:68232/149‑1 (MQ=255)
                                         aaaaaTATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGa                                                                                                                                               <  4:56726/113‑1 (MQ=255)
                                         aaaaaTATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAgg                                                                                                           >  5:106244/1‑149 (MQ=255)
                                          aaaaTATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCCTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCAACGTATTAATTACGATGGGCAATTTGATCCTGAAAAACTACAAACCTCAggt                                                                                                          >  7:233367/1‑149 (MQ=255)
                                                  aCATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTgctg                                                                                                   <  3:102296/148‑1 (MQ=255)
                                                  aCATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTgct                                                                                                    <  2:377742/147‑1 (MQ=255)
                                                    aTCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACaaa                                                                                                                  <  1:231119/131‑1 (MQ=255)
                                                    aTCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAggt                                                                                                          >  8:111017/1‑139 (MQ=255)
                                                    aTCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAggt                                                                                                          <  7:111017/139‑1 (MQ=255)
                                                       gttgttTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGc                                                                                              <  3:41741/148‑1 (MQ=255)
                                                        ttgttTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCg                                                                                            >  1:329001/1‑149 (MQ=255)
                                                           ttttCAATCTGCCGTGTCTGGGATTGACCGGTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAg                                                                                         <  6:125712/149‑1 (MQ=255)
                                                           tttgCAATCTGCCGTGTATGGGATTGACCGTTTTCTTTTGACCCGGAGATCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCa                                                                                          <  5:165830/148‑1 (MQ=255)
                                                            tttCAATCTGCCGTTTATTGTATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAg                                                                                         <  3:43303/148‑1 (MQ=255)
                                                                aaTCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATc                                                                                    >  1:107362/1‑149 (MQ=255)
                                                                      ccGTTTATGGGATTGACCGTTTTCTTTTGACACTGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTcc                                                                                <  1:277123/147‑1 (MQ=255)
                                                                      ccGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTcc                                                                                <  1:406423/147‑1 (MQ=255)
                                                                      ccGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTc                                                                                 <  8:32571/146‑1 (MQ=255)
                                                                      ccGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCtg                                                                              <  8:122411/149‑1 (MQ=255)
                                                                      ccGGTTATTGGATTGTCCGTCTTCTTTTGACACGGCGTTCAACAATGCTCGGAATAATTCTATCTGTCATCGTATTAATTACTATGGGCTATTTGATACTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCtg                                                                              <  6:227154/149‑1 (MQ=255)
                                                                        gTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTc                                                                                 <  6:8233/144‑1 (MQ=255)
                                                                        gTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCtgat                                                                            >  1:393708/1‑149 (MQ=255)
                                                                           tATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGACCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCtgatga                                                                          <  5:91379/148‑1 (MQ=255)
                                                                            aTGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTgatgat                                                                         <  2:269208/148‑1 (MQ=255)
                                                                             tGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGAtgt                                                                       >  6:412158/1‑149 (MQ=255)
                                                                                gATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTAt                                                                                     <  1:46743/132‑1 (MQ=255)
                                                                                gATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTAt                                                                                     <  3:389171/132‑1 (MQ=255)
                                                                                gATTGACAGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTAt                                                                                     >  2:46743/1‑132 (MQ=255)
                                                                                 aTTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCgg                                                                   <  7:268583/149‑1 (MQ=255)
                                                                                 aTTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCgg                                                                   >  8:159552/1‑149 (MQ=255)
                                                                                  ttGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGgt                                                                  <  3:82040/149‑1 (MQ=255)
                                                                                    gACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTgctggc                                                                                                 >  6:53046/1‑116 (MQ=255)
                                                                                     aCCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTTTCATCGTATTAATTACGa                                                                                                                                               <  1:255153/69‑1 (MQ=255)
                                                                                     aCCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGgtgt                                                                <  6:84520/148‑1 (MQ=255)
                                                                                     aCCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTc                                                               >  7:271855/1‑149 (MQ=255)
                                                                                     aCCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTc                                                               >  2:60481/1‑149 (MQ=255)
                                                                                     aCCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTc                                                               >  2:124533/1‑149 (MQ=255)
                                                                                     aCCGTTTTCTTTTGACACGGAGTTCAACAATGTTAGGCATAATTATATCTGTCATCTTATTAATTACGa                                                                                                                                               >  2:255153/1‑69 (MQ=255)
                                                                                      ccGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCt                                                              >  5:198658/1‑149 (MQ=255)
                                                                                                ttGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAggg                                                      <  5:289103/147‑1 (MQ=255)
                                                                                                  gACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCg                                                  >  3:50172/1‑149 (MQ=255)
                                                                                                   acacGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCgg                                                 >  2:302215/1‑149 (MQ=255)
                                                                                                     acGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTTTTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCggtg                                               <  5:89272/149‑1 (MQ=255)
                                                                                                     acGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCggtg                                               <  8:127790/149‑1 (MQ=255)
                                                                                                     acGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCggtg                                               <  6:106244/149‑1 (MQ=255)
                                                                                                      cGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCggtgg                                              >  4:19378/1‑149 (MQ=255)
                                                                                                       ggAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAgg                                                       >  8:425531/1‑139 (MQ=255)
                                                                                                       ggAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAgg                                                       <  7:425531/139‑1 (MQ=255)
                                                                                                        gAGTTCAACAATGTTCGGCATAATTATATCTGTCATCTTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGGATCTTCCTTATGATGTGCGGTGTCTGGTTAGGGTTCggtgggg                                            >  6:57716/1‑147 (MQ=255)
                                                                                                           ttCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCg                                                                    >  4:185805/1‑122 (MQ=255)
                                                                                                           ttCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCg                                                                    <  3:185805/122‑1 (MQ=255)
                                                                                                           ttCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGCTGATGGCTGCCGCAGGTATCTTCATGATTATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTAc                                         >  4:67277/1‑149 (MQ=255)
                                                                                                             caacaaTGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACt                                        <  5:412158/148‑1 (MQ=255)
                                                                                                             caacaaTGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACt                                        <  2:230012/148‑1 (MQ=255)
                                                                                                                  ttGTTCTGCATAATTATATCTGTCATCGTATTTATTTCGATGGGCTATTTGATGGTTTAAAACTACTAACCGAATGTGGTTCTTGCTGCCGCAGGTTTCTTCCTGATGATTTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATcc                                  <  3:67277/148‑1 (MQ=255)
                                                                                                                        ggCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTTGTTAGGGTTCGGTGGTGTACTCGATCacacca                             >  5:60419/1‑148 (MQ=255)
                                                                                                                         gCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGATAGGGTTCGGTGGTGTACTCGATCCCACCaa                            >  1:281366/1‑148 (MQ=255)
                                                                                                                          gaTAATTATATCTGTCATCGTATTAATGTCGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCaa                            >  1:89665/2‑147 (MQ=255)
                                                                                                                               ttATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGc                                                                     >  3:201739/1‑101 (MQ=255)
                                                                                                                               ttATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAagcagc                     <  2:393708/149‑1 (MQ=255)
                                                                                                                                 atatCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACTAACCGCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTTggg                                                      >  3:11946/1‑114 (MQ=255)
                                                                                                                                 atatCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAggg                                                      <  4:11946/114‑1 (MQ=255)
                                                                                                                                  tatCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCgg                   <  7:159552/148‑1 (MQ=255)
                                                                                                                                     cTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTAc               >  8:377264/1‑149 (MQ=255)
                                                                                                                                      tGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACt              >  5:315927/1‑149 (MQ=255)
                                                                                                                                      tGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACt              >  4:156456/1‑149 (MQ=255)
                                                                                                                                      tGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACt              >  7:266492/1‑149 (MQ=255)
                                                                                                                                      tGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGAGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACt              >  3:47968/1‑149 (MQ=255)
                                                                                                                                      gcccATCGTAATTAATACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCccacc                              >  5:247034/4‑133 (MQ=255)
                                                                                                                                      gcccATCGTAATTAATACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCccacc                              <  6:247034/130‑1 (MQ=255)
                                                                                                                                       cccATCGTAATTAATACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACtt             >  4:40001/3‑149 (MQ=255)
                                                                                                                                        tCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCggtggt                                             >  8:191046/1‑116 (MQ=255)
                                                                                                                                           tCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGAtc         >  2:160950/1‑149 (MQ=255)
                                                                                                                                            cGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCt                                                                               <  6:87527/78‑1 (MQ=255)
                                                                                                                                            cGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCt                                                                               >  5:87527/1‑78 (MQ=255)
                                                                                                                                              taattaatacGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGAtcgtc      <  3:40001/147‑1 (MQ=255)
                                                                                                                                              tATTAATTACGATGGGCTATTTGATCCTGAAACACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGAtcgtc      >  3:106818/1‑149 (MQ=255)
                                                                                                                                              tATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGAtcgtc      <  6:315927/149‑1 (MQ=255)
                                                                                                                                               ttttaattaTGATGGGATATTTGATCCTGAATAACTACAAACCTGAGGTGGTGCTGGCTGCCGCATGTATCTTAGTGTTGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCACACCAAAAGCAGCGGCTACTTGAtcgtc      <  4:48844/147‑1 (MQ=255)
                                                                                                                                                attaattaCGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGgt                                                                  <  3:235410/88‑1 (MQ=255)
                                                                                                                                                attaattaCGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGgt                                                                  >  4:235410/1‑88 (MQ=255)
                                                                                                                                                 ttaattaCGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGa    >  3:162989/1‑149 (MQ=255)
                                                                                                                                                 ttaattaCGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGa    <  4:162989/149‑1 (MQ=255)
                                                                                                                                                   aattaCGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGata  >  4:213179/1‑149 (MQ=255)
                                                                                                                                                   aattaCGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGGGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGat   <  6:198658/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACAATTAAAAACACGACATCCGCACCATAAATAGCCTTGCAAAAAATATAACATCGTTGTTTTCAATCTGCCGTTTATGGGATTGACCGTTTTCTTTTGACACGGAGTTCAACAATGTTCGGCATAATTATATCTGTCATCGTATTAATTACGATGGGCTATTTGATCCTGAAAAACTACAAACCTCAGGTGGTGCTGGCTGCCGCAGGTATCTTCCTGATGATGTGCGGTGTCTGGTTAGGGTTCGGTGGTGTACTCGATCCCACCAAAAGCAGCGGCTACTTGATCGTCGATA  >  NC_000913/3374755‑3375049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: