Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,522,154 Δ1 bp 5.1% coding (598/780 nt) hofM ← DNA catabolic putative pilus assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,522,1540C. 5.1% 268.6 / 7.7 78coding (598/780 nt)hofMDNA catabolic putative pilus assembly protein
Reads supporting (aligned to +/- strand):  ref base C (37/37);  new base . (2/2);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

ATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACA  >  NC_000913/3522006‑3522299
                                                                                                                                                    |                                                                                                                                                 
aTGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCAc                                                                                                                                                   <  8:186929/149‑1 (MQ=255)
 tGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCAcc                                                                                                                                                  >  2:330882/1‑149 (MQ=255)
   gCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGCCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGc                                                                                                                                                <  6:41266/149‑1 (MQ=255)
     aaaGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGc                                                                                                                                                >  3:242441/1‑147 (MQ=255)
     aaaGGCTCCTCCTGGCGGCGGTAGCGGCGGCTGACGAACCGAACCGGCCTCCCAGGGTTCAAATCCGCCTTCGCCACATCTCGCGCCGCTTTCGGGATCAACGGATAACGGTGCCTCCAGCTCCTTCGCGCTAGGCATCCCCACCGcga                                                                                                                                              <  6:241043/149‑3 (MQ=255)
        gTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGACGCCAGCGCCTTCGCGCTAGTCATACCCCCCGCCAGtt                                                                                                                                           >  3:174402/1‑149 (MQ=255)
           tcCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTgc                                                                                                                                        >  2:40335/1‑149 (MQ=255)
            ccACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTgcg                                                                                                                                       >  7:209726/1‑149 (MQ=255)
                cTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGcccc                                                                                                                                   >  4:82023/1‑149 (MQ=255)
                  ggcggcGGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGcccc                                                                                                                                   >  8:319849/1‑147 (MQ=255)
                   gcggcgGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCa                                                                                                                                 <  8:128430/148‑1 (MQ=255)
                   gcggcgGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCa                                                                                                                                 <  2:208894/148‑1 (MQ=255)
                   gcggcgGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAg                                                                                                                                <  2:331957/149‑1 (MQ=255)
                   gcggcgGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAg                                                                                                                                >  4:113853/1‑149 (MQ=255)
                         gTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATACCCACCGCCAGTTTGCGCCCCCAGCTATAg                                                                                                                          >  4:37542/1‑149 (MQ=255)
                          tAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAgc                                                                                                                         >  2:177962/1‑149 (MQ=255)
                          tAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAgc                                                                                                                         <  3:82023/149‑1 (MQ=255)
                          tAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAgc                                                                                                                         <  1:177962/149‑1 (MQ=255)
                                 ggcTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGc                                                                                                                  <  6:275060/149‑1 (MQ=255)
                                 ggcTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGc                                                                                                                  <  7:319849/149‑1 (MQ=255)
                                   cTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGccc                                                                                                                <  1:325640/149‑1 (MQ=255)
                                    tGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCa                                                                                                                                              >  3:6796/1‑118 (MQ=255)
                                    tGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCa                                                                                                                                              <  4:6796/118‑1 (MQ=255)
                                    tGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCca                                                                                                               >  6:93692/1‑149 (MQ=255)
                                      aCGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCcaca                                                                                                             <  3:167861/149‑1 (MQ=255)
                                      aCGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGGCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCcaca                                                                                                             <  7:139600/149‑1 (MQ=255)
                                        gAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGc                                                                                                           >  4:192434/1‑149 (MQ=255)
                                         aaCAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGc                                                                                                           >  4:287070/1‑148 (MQ=255)
                                                 cGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCg                                                                                                  <  6:283527/149‑1 (MQ=255)
                                                     cTCCCAGGGATCAAATCCGCCTTCGCCACATCTCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTGTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTAt                                                                                              <  2:50405/149‑1 (MQ=255)
                                                      tCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCTACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTAt                                                                                              >  7:108131/1‑148 (MQ=255)
                                                      tCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGACAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATc                                                                                             >  4:203126/1‑149 (MQ=255)
                                                        ccAGGGCTCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGTGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCa                                                                                            <  2:246884/148‑1 (MQ=255)
                                                             gATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACgccag                                                                                      >  3:242001/1‑149 (MQ=255)
                                                             gATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGAGCGTCGCCCACAGCCACTGTTCGTTATCACgccag                                                                                      >  2:125294/1‑149 (MQ=255)
                                                              aTCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCcaca                                                                                                             <  6:148394/125‑1 (MQ=255)
                                                                   aTCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGa                                                                                <  1:40335/149‑1 (MQ=255)
                                                                     ccGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCA‑CGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACAt                                                                             >  2:265261/1‑149 (MQ=255)
                                                                     ccGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCA‑CGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACAt                                                                             >  2:260797/1‑149 (MQ=255)
                                                                      cGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACa                                                                              <  1:330882/148‑1 (MQ=255)
                                                                         cTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGc                                                                          >  3:48309/1‑149 (MQ=255)
                                                                             gCCACATATCGCGCCGCTTTCGGGATCAACTGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCtgat                                                                      >  8:248392/1‑149 (MQ=255)
                                                                              ccACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCtgatg                                                                     <  2:340152/149‑1 (MQ=255)
                                                                              ccACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCtgat                                                                      >  8:43175/1‑148 (MQ=255)
                                                                               cacaTATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCtgatga                                                                    <  7:269638/149‑1 (MQ=255)
                                                                               cacaTATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCtgatga                                                                    <  3:287070/149‑1 (MQ=255)
                                                                                 caTATCGCGTCGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATgaga                                                                  <  4:242001/149‑1 (MQ=255)
                                                                                  atatCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATgag                                                                   >  2:16190/1‑147 (MQ=255)
                                                                                           cGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTaaaaaa                                                        <  1:16190/149‑1 (MQ=255)
                                                                                           cGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTaaaaaa                                                        <  3:113853/149‑1 (MQ=255)
                                                                                              tttCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCCCAGCCACTGTTCGTTATCACGCCCGGCCAGACATTGCTGATGAGAAGGTAAAAAAgg                                                      >  6:297211/1‑148 (MQ=255)
                                                                                              tttCGGGATCAACGGATAACGATGCCGCCAGCTCCTTCGCGATAGACATCCCCACCGCAATTTAGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCCCTGTTCGATATCACCCAAGGCCACTCATTGCTTAAGACAAGGTAAAAAAgg                                                      >  6:2786/1‑148 (MQ=255)
                                                                                                tCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGc                                                     <  4:242441/147‑1 (MQ=255)
                                                                                                tCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCa                                                    <  5:2169/148‑1 (MQ=255)
                                                                                                  gggATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAgg                                                  >  1:206137/1‑148 (MQ=255)
                                                                                                   ggATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGCCATTGTTGATGAGAAGGTAAAAAAGGCAGGaa                                                >  1:75366/1‑149 (MQ=255)
                                                                                                     aTCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGCTAAAAAAGGCAGGAATc                                              >  7:321091/1‑149 (MQ=255)
                                                                                                      tCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCTTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCg                                             <  3:37542/149‑1 (MQ=255)
                                                                                                      tCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCg                                             <  5:93692/149‑1 (MQ=255)
                                                                                                        aaCGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCt                                           >  1:22675/1‑149 (MQ=255)
                                                                                                          cGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGt                                         <  4:129587/149‑1 (MQ=255)
                                                                                                          cGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCA‑CGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTa                                        <  1:265261/149‑1 (MQ=255)
                                                                                                          cGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCA‑CGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTa                                        <  1:260797/149‑1 (MQ=255)
                                                                                                            gATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTaa                                       <  8:16963/149‑1 (MQ=255)
                                                                                                                 cGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCAc                                  >  7:234282/1‑149 (MQ=255)
                                                                                                                    tGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTgg                               >  2:57354/1‑149 (MQ=255)
                                                                                                                              tCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTAACACGCCAGGCCAGACATTGCTGATGAG‑ACTTAAAAAACGCCGGCATCCCTGTAAATCACAGGCATACCGGGt                     >  8:290664/1‑148 (MQ=255)
                                                                                                                                cTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCACCAGCATTAGCGCGTCGCCCACAGCCAGTGTTCGTTATCACGCCAGGCCAGCCATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTg                    >  1:120510/1‑148 (MQ=255)
                                                                                                                                 ttCGCGCTAGTCATCCCCACCGCCAGTTTGAGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGAt                  >  6:20828/1‑149 (MQ=255)
                                                                                                                                 ttCGCGCTAGTCATACCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTTCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGAt                  >  6:255198/1‑149 (MQ=255)
                                                                                                                                   cgcgCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTa                                                             >  7:295119/1‑104 (MQ=255)
                                                                                                                                   cgcgCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTa                                                             <  8:295119/104‑1 (MQ=255)
                                                                                                                                    gcgcTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGCCATTGTTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATcg                <  2:75366/148‑1 (MQ=255)
                                                                                                                                    gcgcTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATcg                <  3:192434/148‑1 (MQ=255)
                                                                                                                                     cgcTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATcgcg              >  4:304109/1‑149 (MQ=255)
                                                                                                                                     cgcTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATcgcg              <  1:57354/149‑1 (MQ=255)
                                                                                                                                             aTCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACAATGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACAt       >  4:130646/1‑148 (MQ=255)
                                                                                                                                                cccACCTCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACAGTGCGGATGAGAAGGTAAAAAAGGCAGTAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGaa    <  5:291843/148‑1 (MQ=255)
                                                                                                                                                cccACCGCCAGTTGGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGaa    <  1:79317/148‑1 (MQ=255)
                                                                                                                                                 ccACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAaca  >  8:103603/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACA  >  NC_000913/3522006‑3522299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑