Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,926,340 G→A 5.6% M11I (ATG→ATA)  ygdH → UPF0717 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,926,3400GA 5.6% 229.7 / 2.9 71M11I (ATG→ATA) ygdHUPF0717 family protein
Reads supporting (aligned to +/- strand):  ref base G (39/28);  new base A (2/2);  total (41/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

TTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATT  >  NC_000913/2926198‑2926488
                                                                                                                                              |                                                                                                                                                    
ttttttCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATAtg                                                                                                                                                >  4:30918/1‑149 (MQ=255)
     tctcAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTc                                                                                                                                           >  6:1356/1‑149 (MQ=255)
       tcAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTct                                                                                                                                          >  6:49956/1‑147 (MQ=255)
       tcAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCg                                                                                                                                          >  7:214849/1‑148 (MQ=255)
           ttttGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCGTGGCTCCATGGATATGTTGTCGCAGCt                                                                                                                                     <  2:13671/149‑1 (MQ=255)
              tGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTgg                                                                                                                                   <  6:323960/148‑1 (MQ=255)
                 gTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGt                                                                                                                               >  7:214755/1‑149 (MQ=255)
                    cTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGa                                                                                                                            <  8:214849/149‑1 (MQ=255)
                     tGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGa                                                                                                                            >  1:178758/1‑148 (MQ=255)
                         ttCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGc                                                                                                                       >  6:36078/1‑149 (MQ=255)
                            aCGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCtt                                                                                                                     >  1:320260/1‑148 (MQ=255)
                              gCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTaaa                                                                                                                  >  5:210022/1‑149 (MQ=255)
                              gCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTaaa                                                                                                                  <  5:1356/149‑1 (MQ=255)
                                   aGGTTGTGAAAGGTCATCTGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCa                                                                                                              <  4:125772/148‑1 (MQ=255)
                                    ggTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCAcc                                                                                                            >  4:265125/1‑149 (MQ=255)
                                           aaaGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCcag                                                                                                       >  1:249565/1‑147 (MQ=255)
                                           aaaGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATAGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCcag                                                                                                       >  6:226348/1‑147 (MQ=255)
                                           aaaGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATAGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCcag                                                                                                       >  6:226346/1‑147 (MQ=255)
                                            aaGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCcagcag                                                                                                    >  2:238746/1‑149 (MQ=255)
                                               gTCATCAGGCAGGGCGATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGa                                                                                                 <  8:294045/149‑1 (MQ=255)
                                                  atcaGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACct                                                                                              >  5:48636/1‑149 (MQ=255)
                                                  atcaGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACct                                                                                              >  2:177557/1‑149 (MQ=255)
                                                       gcaggGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTAt                                                                                          >  8:126545/1‑148 (MQ=255)
                                                       gcaggGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTAt                                                                                          >  4:122854/1‑148 (MQ=255)
                                                          gggCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAAc                                                                                      >  8:254591/1‑149 (MQ=255)
                                                          gggCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAAc                                                                                      <  2:178758/149‑1 (MQ=255)
                                                           ggCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACt                                                                                     <  5:36078/149‑1 (MQ=255)
                                                            gCTATTGTAATCAAAGGGAATGCCGATATTCGTCCCATAAGGAGTTTTTCTTGATAACACATATTAGCCCGCTTGGCTCCATGGATATCTTGTCGCAGCTGGAAGTGGATATGCTTAAAAGCACCGCCAGCAGCGACCACTATCAACTg                                                                                    >  6:143788/1‑149 (MQ=255)
                                                            gCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTg                                                                                    >  2:220795/1‑149 (MQ=255)
                                                                   tAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGc                                                                              >  8:145642/1‑148 (MQ=255)
                                                                     aTCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCaa                                                                            <  7:6218/148‑1 (MQ=255)
                                                                      tCAAAGGGAATGACTATAGTCGGCCAATAAGGAGTTTTTCTTGATTACCCATATTAGCCCGCGTGGCTCCATGGATCTGTTGTCGCTGCTGGAAGTGGATATGATTAACCGCACCGCAAGCAGCGACCTCTATCAAGTGTTTCGCaagt                                                                          >  2:181067/1‑147 (MQ=255)
                                                                         aaGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGtt                                                                       >  4:56625/1‑149 (MQ=255)
                                                                                tGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGc                                                                >  8:70692/1‑149 (MQ=255)
                                                                                 gACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATc                                                                                         >  3:226118/1‑123 (MQ=255)
                                                                                 gACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATc                                                                                         <  4:226118/123‑1 (MQ=255)
                                                                                     atatTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTAc                                                           <  8:214755/149‑1 (MQ=255)
                                                                                     atatTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTAc                                                           <  3:289707/149‑1 (MQ=255)
                                                                                     atatTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTAc                                                           <  3:30918/149‑1 (MQ=255)
                                                                                            tCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGACCTc                                                    >  5:204442/1‑149 (MQ=255)
                                                                                            tCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTc                                                    >  4:179599/1‑149 (MQ=255)
                                                                                            tCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTc                                                    <  7:254591/149‑1 (MQ=255)
                                                                                              ccATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCg                                                  <  1:213835/149‑1 (MQ=255)
                                                                                              ccATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCCACCTCTATCAACTGTTTCGCAACTGTACACTTGCCGTACTGAACACCg                                                  >  6:14536/1‑149 (MQ=255)
                                                                                                aTAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGt                                                <  1:274661/149‑1 (MQ=255)
                                                                                                 tAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTa                                               <  3:265125/149‑1 (MQ=255)
                                                                                                    ggAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGtt                                            <  2:249565/149‑1 (MQ=255)
                                                                                                    ggAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGtt                                            <  6:210022/149‑1 (MQ=255)
                                                                                                       gTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGa                                         >  7:142279/1‑149 (MQ=255)
                                                                                                              ttGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGt                                                                                                                               <  4:169726/56‑1 (MQ=255)
                                                                                                              ttGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGt                                                                                                                               >  3:169726/1‑56 (MQ=255)
                                                                                                              ttGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATaa                                  >  1:185474/1‑149 (MQ=255)
                                                                                                                 aTTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACa                                <  3:122854/148‑1 (MQ=255)
                                                                                                                 aTTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAg                               >  5:272721/1‑149 (MQ=255)
                                                                                                                    acacATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCa                             >  6:74514/1‑148 (MQ=255)
                                                                                                                     cacaTATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGt                                             >  6:147784/1‑131 (MQ=255)
                                                                                                                     cacaTATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGt                                             <  5:147784/131‑1 (MQ=255)
                                                                                                                     cacaTATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCaaa                           <  7:70692/149‑1 (MQ=255)
                                                                                                                     cacaTATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCaaa                           <  5:163928/149‑1 (MQ=255)
                                                                                                                      acaTATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGGAGTTTGACCGATAACAGCa                             >  2:310532/1‑146 (MQ=255)
                                                                                                                        atatTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGaa                        >  6:328166/1‑149 (MQ=255)
                                                                                                                        atatTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGaa                        >  4:175320/1‑149 (MQ=255)
                                                                                                                         tatTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCca                                                                                                        >  2:116902/1‑68 (MQ=255)
                                                                                                                         tatTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAAt                       >  6:201659/1‑149 (MQ=255)
                                                                                                                             aGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGc                    <  8:13482/148‑1 (MQ=255)
                                                                                                                              gCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTg                  <  5:49956/149‑1 (MQ=255)
                                                                                                                              gCCCGCTTGGCTCCATAGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCt                   <  5:226348/148‑1 (MQ=255)
                                                                                                                              gCCCGCTTGGCTCCATAGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCt                   <  5:226346/148‑1 (MQ=255)
                                                                                                                                  gCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGtctc              <  1:220795/149‑1 (MQ=255)
                                                                                                                                       gatacATGGATATGTTGTCTCAGCTGGAAGTGGATATGCTTAAACGCACCGCCACCAGCGACCTCTATCAACTGTTTCTCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAACAATTGCTGTCTCGttt          <  6:87328/144‑1 (MQ=255)
                                                                                                                                           cATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGaa      >  1:198518/1‑148 (MQ=255)
                                                                                                                                              ggATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAAtt  >  7:265885/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
TTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATT  >  NC_000913/2926198‑2926488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑