Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,105,959 G→A 5.2% T82T (ACG→ACA)  nupG → nucleoside transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,105,9590GA 5.2% 245.1 / 2.9 77T82T (ACG→ACA) nupGnucleoside transporter
Reads supporting (aligned to +/- strand):  ref base G (37/35);  new base A (2/2);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

AAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGA  >  NC_000913/3105813‑3106105
                                                                                                                                                  |                                                                                                                                                  
aaGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACg                                                                                                                                                    <  6:191230/147‑1 (MQ=255)
aaGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCt                                                                                                                                                  <  2:310266/149‑1 (MQ=255)
  gTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACACTGt                                                                                                                                                >  8:172991/1‑149 (MQ=255)
  gTTTGAAGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATAGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACAACATTGTCGCTATCACACTGt                                                                                                                                                >  8:177474/1‑149 (MQ=255)
       aCGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATg                                                                                                                                           >  5:207387/1‑149 (MQ=255)
        cGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATgg                                                                                                                                          >  7:266341/1‑149 (MQ=255)
           tGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATggcgg                                                                                                                                       <  6:207387/149‑1 (MQ=255)
           tGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATggcgg                                                                                                                                       <  3:239825/149‑1 (MQ=255)
              ttCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGca                                                                                                                                     >  5:261853/1‑148 (MQ=255)
                cTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGcac                                                                                                                                    <  2:155037/147‑1 (MQ=255)
                   ttGGCGCAGTTTATAGCGCACTGGGTATCGCCGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTGAAGTGCGAAATGTGTCTATGCCATTTGCCACACCATTGGCGCTATCACGcgggtc                                                                                                                                              >  4:233189/1‑129 (MQ=255)
                      gcgcAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCAATGGCGCTATCACGCTGTTCATGGCGGCACAGGTCAc                                                                                                                             <  6:210909/148‑1 (MQ=255)
                        gcAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTc                                                                                                                               <  3:152756/144‑1 (MQ=255)
                        gcAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTc                                                                                                                               >  4:152756/1‑144 (MQ=255)
                            tttATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCg                                                                                                                      >  5:83122/1‑149 (MQ=255)
                            tttATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGACACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCg                                                                                                                      >  5:218562/1‑149 (MQ=255)
                               ataGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCgg                                                                                                                     >  5:273522/1‑147 (MQ=255)
                                taGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAg                                                                                                                  <  2:102676/149‑1 (MQ=255)
                                  gCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCa                                                                                                                                             >  1:326183/1‑120 (MQ=255)
                                  gCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGc                                                                                                                 >  7:211998/1‑148 (MQ=255)
                                  gCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCg                                                                                                                <  8:165474/149‑1 (MQ=255)
                                        tGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTc                                                                                                          >  4:83150/1‑149 (MQ=255)
                                              tCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTtgt                                                                                                     <  6:273522/148‑1 (MQ=255)
                                                gCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGa                                                                                                   >  3:197453/1‑148 (MQ=255)
                                                 cAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGata                                                                                                 >  3:73633/1‑149 (MQ=255)
                                                      gTCTTTATGCCTGCGCTGCTGGGGATTGTGTCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCAAACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGAt                                                                                            <  5:292637/149‑1 (MQ=255)
                                                        cTTTATGACTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGAGCTATCACGCTGTTCATGGCGTCACAGGTCACGACACCGGCAGCGATGTTCCTTGTGATATTGATTa                                                                                          <  2:187633/149‑1 (MQ=255)
                                                         tttATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTaa                                                                                         >  6:272242/1‑149 (MQ=255)
                                                               ccTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGtt                                                                                   >  8:10155/1‑149 (MQ=255)
                                                               ccTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGtt                                                                                   >  1:151283/1‑149 (MQ=255)
                                                                cTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGttt                                                                                  <  3:83150/149‑1 (MQ=255)
                                                                 tGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAATCGATGTTCCTTGTGATATTGATTAACTCGTTTg                                                                                 >  3:165801/1‑149 (MQ=255)
                                                                   cgcTGCTGGGGATTGTGGCCGACACATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGAGGTTCCTTGTGATATTGATTAACTCGTTTGc                                                                                <  6:223629/148‑1 (MQ=255)
                                                                   cgcTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTg                                                                                 <  3:157585/147‑1 (MQ=255)
                                                                   cgcTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGc                                                                                <  7:236629/148‑1 (MQ=255)
                                                                   cgcTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGc                                                                                <  8:266341/148‑1 (MQ=255)
                                                                    gctgctGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCtt                                                                              <  6:83122/149‑1 (MQ=255)
                                                                                  tgGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCtt                                                                >  7:300259/1‑149 (MQ=255)
                                                                                    gCCGACAAATTGGTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCTGCAAAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTgg                                                              <  5:65130/149‑1 (MQ=255)
                                                                                    gCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTgg                                                              <  4:292726/149‑1 (MQ=255)
                                                                                    gCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTgg                                                              <  5:165796/149‑1 (MQ=255)
                                                                                    gCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTgg                                                              <  6:261853/149‑1 (MQ=255)
                                                                                     ccGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTtata                                                                           <  7:330220/135‑1 (MQ=255)
                                                                                     ccGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTtata                                                                           >  8:330220/1‑135 (MQ=255)
                                                                                     ccGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTggg                                                             <  8:300259/149‑1 (MQ=255)
                                                                                       gACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGt                                                            >  4:59012/1‑148 (MQ=255)
                                                                                          aaaTGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAAt                                                        >  5:18245/1‑149 (MQ=255)
                                                                                               gTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAaca                                                   >  1:142162/1‑149 (MQ=255)
                                                                                                   aGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCAt                                               <  4:290344/149‑1 (MQ=255)
                                                                                                     tGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCAtct                                             >  3:295549/1‑149 (MQ=255)
                                                                                                     tGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGGTTGCTTATATGCCAACGCTTGGGTTAATCAACACCAtct                                             >  3:37442/1‑149 (MQ=255)
                                                                                                       ctaaaTGGGTATATGCCATTTGCCACAACATTTGCGCTATCACACTGTTCATGGCGGCACAGGTCACGACACCTGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCAtctc                                            <  7:177474/146‑1 (MQ=255)
                                                                                                       cGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATctct                                           >  6:40273/1‑149 (MQ=255)
                                                                                                       cGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACACTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCAtctc                                            <  7:172991/148‑1 (MQ=255)
                                                                                                               gTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCg                                   >  2:160003/1‑149 (MQ=255)
                                                                                                               gTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCg                                   <  7:10155/149‑1 (MQ=255)
                                                                                                                   atGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTgg                                                              >  2:298025/1‑118 (MQ=255)
                                                                                                                   atGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTgg                                                              <  1:298025/118‑1 (MQ=255)
                                                                                                                     gCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGc                              >  8:143570/1‑148 (MQ=255)
                                                                                                                      ccATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCa                             >  4:298794/1‑148 (MQ=255)
                                                                                                                        aTTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCaaaa                          <  5:272242/149‑1 (MQ=255)
                                                                                                                        aTTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACGCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCaaaa                          <  4:113047/149‑1 (MQ=255)
                                                                                                                               acacCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGttt                                                                                  <  7:77568/86‑1 (MQ=255)
                                                                                                                               acacCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGttt                                                                                  >  8:77568/1‑86 (MQ=255)
                                                                                                                                cacCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGgga                  >  5:200915/1‑149 (MQ=255)
                                                                                                                                cacCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATACCGgga                  >  3:199675/1‑149 (MQ=255)
                                                                                                                                  ccATTGGAGCTATCACTCTGTTCATGGAGGATCAGGTCCCGACACCGGAATCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCATGCAAAATGCCGggatg                <  4:179174/149‑1 (MQ=255)
                                                                                                                                    aTTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGggatgga              <  4:295549/149‑1 (MQ=255)
                                                                                                                                     ttGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGggatggat             <  4:156122/149‑1 (MQ=255)
                                                                                                                                        gcgcTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGatat           <  4:169275/148‑1 (MQ=255)
                                                                                                                                            tATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTa      >  5:68334/1‑149 (MQ=255)
                                                                                                                                             aTCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGtt                                                                                   <  6:306923/71‑1 (MQ=255)
                                                                                                                                             aTCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGtt                                                                                   >  5:306923/1‑71 (MQ=255)
                                                                                                                                              tCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACt    <  6:200915/149‑1 (MQ=255)
                                                                                                                                                aCGCTGTTCCTGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGa  >  5:296610/1‑149 (MQ=255)
                                                                                                                                                aCGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTCCTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGa  >  4:183932/1‑149 (MQ=255)
                                                                                                                                                aCGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGa  <  8:274217/149‑1 (MQ=255)
                                                                                                                                                aCGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGGTAATCAACACCATCACTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGa  >  2:198994/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAGTTTGACGGTGCTTCTATTGGCGCAGTTTATAGCTCACTGGGTATCGCAGCGGTCTTTATGCCTGCGCTGCTGGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGA  >  NC_000913/3105813‑3106105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑