Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 396,037 C→T 8.0% R84H (CGT→CAT)  ampH ← D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase; penicillin‑binding protein; weak beta‑lactamase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913396,0370CT 8.0% 230.2 / 6.5 75R84H (CGT→CAT) ampHD‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase; penicillin‑binding protein; weak beta‑lactamase
Reads supporting (aligned to +/- strand):  ref base C (39/30);  new base T (3/3);  total (42/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTGGCACCGCTGCCGTAAAAAATATGATCGGCATAACGATC  >  NC_000913/395890‑396182
                                                                                                                                                   |                                                                                                                                                 
aCCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACg                                                                                                                                                  >  7:252552/1‑149 (MQ=255)
   aGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTTGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTAGGTGAGGTAAGCAATACGCAc                                                                                                                                               >  2:230216/1‑149 (MQ=255)
       tAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGAcc                                                                                                                                           >  3:16305/1‑149 (MQ=255)
          tGGGCGTCCCATTGTATGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTTACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGGTTGGTGAGGGAAGCAATACGCCCGACCGaa                                                                                                                                        >  6:93740/1‑149 (MQ=255)
          tGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGGCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGATGCAATACGCCCGACCgca                                                                                                                                        >  6:262158/1‑147 (MQ=255)
                tCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGc                                                                                                                                  >  1:27905/1‑149 (MQ=255)
                 cccATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCt                                                                                                                                 >  2:159336/1‑149 (MQ=255)
                   cATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGc                                                                                                                               >  5:192429/1‑149 (MQ=255)
                    aTTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCg                                                                                                                              <  2:118073/149‑1 (MQ=255)
                     ttGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGGCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGACATTCGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCgg                                                                                                                             <  5:32671/149‑1 (MQ=255)
                         aGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCCGCTGCGGGCg                                                                                                                          >  6:304657/1‑148 (MQ=255)
                          ggTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGaa                                                                                                                        <  1:159336/149‑1 (MQ=255)
                           gTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAAc                                                                                                                       >  2:315547/1‑149 (MQ=255)
                                 aCGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACAGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGttat                                                                                                                  >  2:228320/1‑148 (MQ=255)
                                     gTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCa                                                                                                             <  4:16305/149‑1 (MQ=255)
                                     gTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCa                                                                                                             <  3:253969/149‑1 (MQ=255)
                                      tGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAg                                                                                                            <  7:192913/149‑1 (MQ=255)
                                         gcCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTc                                                                                                         >  3:6302/1‑149 (MQ=255)
                                            tGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTcgcg                                                                                                      >  8:65872/1‑149 (MQ=255)
                                             ggCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGt                                                                                                     <  8:294757/149‑1 (MQ=255)
                                                 gAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAACACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCg                                                                                                 >  5:282884/1‑149 (MQ=255)
                                                     atatTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAACACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCAt                                                                                             <  6:282884/149‑1 (MQ=255)
                                                      tatTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGaa                                                                                                                        >  3:155480/1‑121 (MQ=255)
                                                      tatTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGaa                                                                                                                        <  4:155480/121‑1 (MQ=255)
                                                        tttACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATaa                                                                                           <  8:252552/148‑1 (MQ=255)
                                                            cTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCg                                                                                      <  6:55203/149‑1 (MQ=255)
                                                            cTTAGCGGATCGTTTAACTTCACGGGCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGc                                                                                       <  4:26509/148‑1 (MQ=255)
                                                             ttAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCg                                                                                      >  8:266960/1‑148 (MQ=255)
                                                             ttAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCg                                                                                      >  1:72511/1‑148 (MQ=255)
                                                                gCGGATCGTTTCACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGaaa                                                                                   <  2:316499/148‑1 (MQ=255)
                                                                     tCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGAcgcg                                                                             <  8:233681/149‑1 (MQ=255)
                                                                       gTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAGGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCt                                                                           >  6:108629/1‑149 (MQ=255)
                                                                       gTTTAACTTCACGGTCCCCCGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCt                                                                           <  3:16955/149‑1 (MQ=255)
                                                                        tttAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTg                                                                          <  1:160503/149‑1 (MQ=255)
                                                                        tttAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTg                                                                          <  2:72511/149‑1 (MQ=255)
                                                                              ttCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTAcc                                                                    >  4:29860/1‑149 (MQ=255)
                                                                              ttCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTAcc                                                                    >  5:207055/1‑149 (MQ=255)
                                                                              ttCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACACACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTAcc                                                                    >  2:42671/1‑149 (MQ=255)
                                                                                 aCGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGt                                                                  <  2:27905/148‑1 (MQ=255)
                                                                                 aCGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTc                                                                 <  7:137004/149‑1 (MQ=255)
                                                                                   ggTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAg                                                                                                            >  4:274525/1‑104 (MQ=255)
                                                                                   ggTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAg                                                                                                            <  3:274525/104‑1 (MQ=255)
                                                                                   ggTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGa                                                               <  1:312703/149‑1 (MQ=255)
                                                                                   ggTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCGTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGa                                                               >  2:284946/1‑149 (MQ=255)
                                                                                    gTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGAt                                                              <  1:215789/149‑1 (MQ=255)
                                                                                    gTCCCCTGGTCGAGCAATGTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGGGTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGAt                                                              <  6:180939/149‑1 (MQ=255)
                                                                                     tCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATATGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGAt                                                              >  4:248407/1‑148 (MQ=255)
                                                                                      ccccTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATaa                                                            <  4:88054/149‑1 (MQ=255)
                                                                                          tGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATacccac                                                        >  8:116839/1‑144 (MQ=255)
                                                                                            gTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATaacaac                                                        <  1:190482/147‑1 (MQ=255)
                                                                                             tCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACACACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCag                                                     <  1:42671/149‑1 (MQ=255)
                                                                                                aGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTTCCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGc                                                  >  3:189171/1‑149 (MQ=255)
                                                                                                 gCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGCCGCGCTGGTTACCGTCGATAACAACCAGAGc                                                  >  5:27258/1‑148 (MQ=255)
                                                                                                        cACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCg                                          >  6:143478/1‑149 (MQ=255)
                                                                                                           cAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTgg                                       >  5:49966/1‑149 (MQ=255)
                                                                                                            aGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTGGc                                      >  8:326018/1‑149 (MQ=255)
                                                                                                                  tcACTGGTCATTAGCTTGGTGAGGGAAGCTAAACGCACGACCGAATCAAGCGGAGGGCGACCGTTATTACCAGGTCGCGTTTAGCCATAACTGCGAAAGACTCTCTCGTTACATTCGATAACAACCAGCGCCATCACCGTGGAACAGCt                                >  4:175477/1‑149 (MQ=255)
                                                                                                                  tcACTGGTCATTAGCTTGGTGAGGGAAGCAATATGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATACCTGCGAAAGACGCGCTGGTTa                                                                      <  3:55239/111‑1 (MQ=255)
                                                                                                                  tcACTGGTCATTAGCTTGGTGAGGGAAGCAATATGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTa                                                                      <  3:51020/111‑1 (MQ=255)
                                                                                                                  tcACTGGTCATTAGCTTGGTGAGGGAAGCAATATGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTa                                                                      >  4:55239/1‑111 (MQ=255)
                                                                                                                  tcACTGGTCATTAGCTTGGTGAGGGAAGCAATATGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTa                                                                      >  4:51020/1‑111 (MQ=255)
                                                                                                                        gTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCt                                                                           <  4:289922/100‑1 (MQ=255)
                                                                                                                        gTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCt                                                                           >  3:289922/1‑100 (MQ=255)
                                                                                                                               gCTTGGTGAGGGAAGCAATATGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTGGCACCGCTGCCGTAAAAAat                    <  3:248407/148‑1 (MQ=255)
                                                                                                                                cTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTGGCACCGCTGCCGTAAAAAatat                  >  4:277248/1‑149 (MQ=255)
                                                                                                                                cTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTGGCACCGCTGCCGTAAAAAatat                  >  4:217478/1‑149 (MQ=255)
                                                                                                                                 ttGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTGGCACCGCTGCCGTAAAAAatat                  >  3:307108/1‑148 (MQ=255)
                                                                                                                                   ggTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTGGCACCGCTGCCGTAAAAAATATGAt               >  6:301728/1‑149 (MQ=255)
                                                                                                                                    gTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTGGCACCGCTGCCGTAAAAAATATGATc              >  6:163615/1‑149 (MQ=255)
                                                                                                                                            aGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTGGCACCGCTGCCGTAAAAAATATGATCGGCATAAc      >  2:26398/1‑149 (MQ=255)
                                                                                                                                               aaTACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTGGCACCGCTGCCGTAAAAAATATGATCGGCATAACGAt   <  5:163615/149‑1 (MQ=255)
                                                                                                                                                aTACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTGGCACCGCTGCCGTAAAAAATATGATCGGCATAACGATc  <  6:192429/149‑1 (MQ=255)
                                                                                                                                                aTACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTGGCACCGCTGCCGTAAAAAATATGATCGGCATAACGATc  <  3:29860/149‑1 (MQ=255)
                                                                                                                                                 tACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTGGCACCGCTGCCGTAAAAAATATGATCGGCATAACGATc  >  7:14591/1‑148 (MQ=255)
                                                                                                                                                 tACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTGGCACCGCTGCCGTAAAAAATATGATCGGCATAACGATc  >  7:144082/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGATAACAACCAGAGCCATCCCCGTGGCACCGCTGCCGTAAAAAATATGATCGGCATAACGATC  >  NC_000913/395890‑396182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑