Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	423,329	A→G	6.8%	H271R (CAC→CGC)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	423,329	0	A	G	6.8%	176.4 / 2.6	59	H271R (CAC→CGC)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base A (25/30);  new base G (2/2);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

TGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTC  >  NC_000913/423192‑423477                                                                                                                                          |                                                                                                                                                     tGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAAt                                                                                                                                           >  8:89580/1‑149 (MQ=255)      gggCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGccc                                                                                                                                      >  4:80411/1‑149 (MQ=255)      gggCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGccc                                                                                                                                      <  3:272046/149‑1 (MQ=255)       ggCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGATTGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACCACCCCGAATCGCCCt                                                                                                                                     >  5:128923/1‑149 (MQ=255)        gCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTg                                                                                                                                    <  8:172391/149‑1 (MQ=255)               tgcGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGAACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGAc                                                                                                                             >  3:306347/1‑149 (MQ=255)                     tGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTa                                                                                                                        <  4:287092/148‑1 (MQ=255)                     tGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTa                                                                                                                        <  3:212438/148‑1 (MQ=255)                      gCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACt                                                                                                                      >  6:251633/1‑149 (MQ=255)                         acacAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGt                                                                                                                   <  7:89580/149‑1 (MQ=255)                          cacaATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGtt                                                                                                                  <  1:6705/149‑1 (MQ=255)                           acaATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGttt                                                                                                                 <  8:155648/149‑1 (MQ=255)                            caATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTa                                                                                                                <  4:6673/149‑1 (MQ=255)                             aaTACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAg                                                                                                               >  4:173323/1‑149 (MQ=255)                              aTACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGt                                                                                                              <  3:80411/149‑1 (MQ=255)                               tACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtg                                                                                                             <  1:238446/149‑1 (MQ=255)                               tACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGt                                                                                                              >  3:76802/1‑148 (MQ=255)                                 cGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGtgat                                                                                                           <  7:71402/149‑1 (MQ=255)                                     tcagcTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGatgatg                                                                                                       <  2:130042/148‑1 (MQ=255)                                                tGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCAcaaatcg                                                                                             >  7:71452/1‑142 (MQ=255)                                                         tGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggctt                                                                                   >  1:59622/1‑149 (MQ=255)                                                           cTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggcttgg                                                                                 <  1:276706/149‑1 (MQ=255)                                                           cTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACtggcttg                                                                                  <  3:26699/148‑1 (MQ=255)                                                                    gtgtTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGCCTGGTACTCGTTTAGTGATGATGGCCCGGCGCTCGACTGGCTTGGCTATGCCAg                                                                        >  4:180385/1‑149 (MQ=255)                                                                         tAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGc                                                                   >  6:206374/1‑149 (MQ=255)                                                                          aaCCACCGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGc                                                                   <  3:173323/148‑1 (MQ=255)                                                                          aaCCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGcc                                                                  >  2:75239/1‑149 (MQ=255)                                                                          aaCCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGc                                                                   <  4:306347/148‑1 (MQ=255)                                                                            ccACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGCCTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGa                                                                >  6:185159/1‑149 (MQ=255)                                                                                aGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCt                                                            <  4:153501/149‑1 (MQ=255)                                                                                  tGGCGATTCCCATGCCTGGTTTGAGAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTTGACTGGCTTGGCTATGCCAGCTTGCCGAAGGTgg                                                          <  8:261668/149‑1 (MQ=255)                                                                                           ccATGCCTGGTTTGACAGGCATAATCGTGGCTCGGGTGGGTCTTGTCACAACCCCGAATCGCCCTTGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGa                                                         <  2:202997/141‑1 (MQ=255)                                                                                           ccATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCGCAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGt                                                 >  2:262008/1‑149 (MQ=255)                                                                                           ccATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCGCAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGt                                                 >  2:257628/1‑149 (MQ=255)                                                                                           ccATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGAGTGGTACTCGTATAGTGATGATCGCACGGCGCTCGACTTGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGt                                                 >  7:282827/1‑149 (MQ=255)                                                                                           ccATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGa                                                         >  1:202997/1‑141 (MQ=255)                                                                                           ccATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGt                                                 >  8:322243/1‑149 (MQ=255)                                                                                           ccATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGt                                                 >  7:248050/1‑149 (MQ=255)                                                                                            cATGCCTGGTTTGACAGGCATAATCGTGGCTCGGGGGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTc                                                <  1:180188/149‑1 (MQ=255)                                                                                            cATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTc                                                >  8:34651/1‑149 (MQ=255)                                                                                            cATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGGCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAg                                                              <  2:57986/135‑1 (MQ=255)                                                                                              tGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCgg                                              <  8:248050/149‑1 (MQ=255)                                                                                                 cTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGa                                             >  4:294529/1‑147 (MQ=255)                                                                                                       tGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTg                                      <  3:294529/148‑1 (MQ=255)                                                                                                         aCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGTACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGt                                    <  1:168680/148‑1 (MQ=255)                                                                                                         aCAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGt                                    >  3:292986/1‑148 (MQ=255)                                                                                                                tAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGaaa                            <  4:76802/149‑1 (MQ=255)                                                                                                                tAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGaaa                            <  8:303685/149‑1 (MQ=255)                                                                                                                        gCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGc                                                                   <  7:146760/102‑1 (MQ=255)                                                                                                                        gCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGc                                                                   >  8:146760/1‑102 (MQ=255)                                                                                                                         cACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATcgcg                   <  5:303593/149‑1 (MQ=255)                                                                                                                          aCGGGTGGTGCTTGTCGCAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCgg                  <  1:257628/149‑1 (MQ=255)                                                                                                                          aCGGGTGGTGCTTGTCGCAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCgg                  <  1:262008/149‑1 (MQ=255)                                                                                                                            gggTGGTGCTTGTGACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCgg                  <  1:75239/147‑1 (MQ=255)                                                                                                                             ggtggtGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCgggg                >  1:159442/1‑148 (MQ=255)                                                                                                                              gtggtgCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCgggg                >  1:270864/1‑147 (MQ=255)                                                                                                                                 gtgCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGa            <  7:34651/148‑1 (MQ=255)                                                                                                                                  tgCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGa            >  3:211578/1‑147 (MQ=255)                                                                                                                                        tCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTg    >  3:246124/1‑149 (MQ=255)                                                                                                                                          acaACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTc  >  4:289953/1‑149 (MQ=255)                                                                                                                                          |                                                                                                                                                     TGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCGCGACTGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTC  >  NC_000913/423192‑423477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑