Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,131,440 G→A 5.0% M1M (ATG→ATA) † flgC → flagellar component of cell‑proximal portion of basal‑body rod

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,131,4400GA 5.0% 257.0 / 2.2 79M1M (ATG→ATA) †flgCflagellar component of cell‑proximal portion of basal‑body rod
Reads supporting (aligned to +/- strand):  ref base G (40/35);  new base A (2/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.21e-01

AGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGG  >  NC_000913/1131292‑1131579
                                                                                                                                                    |                                                                                                                                           
aGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATg                                                                                                                                             <  6:323824/149‑1 (MQ=255)
 gCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGGTTTACAGAGCGGAAATTAACGGATgg                                                                                                                                            >  6:266845/1‑149 (MQ=255)
    ttCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTTGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGAGGAAATTAACGGATGGCag                                                                                                                                         >  5:209866/1‑148 (MQ=255)
      cGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCActg                                                                                                                                       >  7:287847/1‑149 (MQ=255)
                tAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATAttt                                                                                                                             >  6:307043/1‑149 (MQ=255)
                  aTACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATAttttt                                                                                                                           <  7:200123/149‑1 (MQ=255)
                  aTACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATAttttt                                                                                                                           <  2:172479/149‑1 (MQ=255)
                     ccGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGa                                                                                                                         >  1:164996/1‑148 (MQ=255)
                     ccGTCGATATGGATCGCGAACGCACCCAGTATGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGa                                                                                                                         >  5:69581/1‑148 (MQ=255)
                      cGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATgg                                                                                                                                            >  3:179633/1‑128 (MQ=255)
                      cGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATgg                                                                                                                                            <  4:179633/128‑1 (MQ=255)
                      cGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGata                                                                                                                       >  3:152513/1‑149 (MQ=255)
                      cGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATAGCACTGCTGAATATTTTTGata                                                                                                                       >  2:202419/1‑149 (MQ=255)
                      cGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATAGCACTGCTGAATATTTTTGata                                                                                                                       >  4:144926/1‑149 (MQ=255)
                       gTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGatat                                                                                                                      <  2:230217/149‑1 (MQ=255)
                               ggATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAATTTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGt                                                                                                              >  2:247899/1‑149 (MQ=255)
                               ggATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGt                                                                                                              >  8:75061/1‑149 (MQ=255)
                                gATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTc                                                                                                             >  2:214671/1‑149 (MQ=255)
                                gATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTc                                                                                                             >  3:136097/1‑149 (MQ=255)
                                    gcgAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCg                                                                                                         <  1:280352/149‑1 (MQ=255)
                                      gAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGt                                                                                                        >  4:154181/1‑148 (MQ=255)
                                      gAACGCACCCAGTTTGCAGATAACAGCCTGCAATACCAGATGAGCCTTAGCGAGTTGAGCGGGCAAATCAAAGGCATGATGAACGTATTACAGAGCGGAAATTAACGGATGGCACTGGTGAATATTTTTGATTAAGCCGGGTCGGCGt                                                                                                        >  8:264402/1‑148 (MQ=255)
                                        aagaaCCAAGTTTGCCGATAACAGCCTGCAATACAAGATGAGACTTCTAGCGGTTATCGGGCAAATCAAATGCATGATGAAAGTTTTACAGAGCGGAAATTAACGGATGTCACTGCTGAATATTTTTGATATCGCAGGGTCGGAGTTaa                                                                                                     <  3:97059/145‑1 (MQ=255)
                                        aCGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTaa                                                                                                     >  5:321711/1‑149 (MQ=255)
                                            aCCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGc                                                                                                 >  7:107514/1‑149 (MQ=255)
                                             cccAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGcc                                                                                                <  5:307043/149‑1 (MQ=255)
                                             cccAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGcc                                                                                                <  8:107514/149‑1 (MQ=255)
                                              ccAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGACCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGt                                                                                                        <  3:102828/140‑1 (MQ=255)
                                              ccAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTAGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGt                                                                                                        >  4:102828/1‑140 (MQ=255)
                                                      ccGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAg                                                                                       >  2:253319/1‑149 (MQ=255)
                                                       cGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAgc                                                                                      >  1:163169/1‑149 (MQ=255)
                                                            aCAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCt                                                                                  <  4:15330/148‑1 (MQ=255)
                                                              aGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCCGCGCCTGat                                                                               >  1:301004/1‑148 (MQ=255)
                                                              aGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGTGCCTGaa                                                                               >  1:320779/1‑149 (MQ=255)
                                                                ccTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACg                                                                             <  4:135396/149‑1 (MQ=255)
                                                                 cTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGt                                                                            >  7:229088/1‑149 (MQ=255)
                                                                 atGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTTCAGAGCGGAAATTAACGGCTGGCACTGCTGAAGATTTTTGCTATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAActg                                                                            >  7:229092/2‑147 (MQ=255)
                                                                    cAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTg                                                                           <  3:154181/147‑1 (MQ=255)
                                                                       tACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTggcggc                                                                      >  1:160060/1‑149 (MQ=255)
                                                                          cAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCa                                                                                                                                          >  4:13571/1‑78 (MQ=255)
                                                                          cAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCa                                                                                                                                          <  7:55689/78‑1 (MQ=255)
                                                                          cAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCa                                                                                                                                          <  3:13571/78‑1 (MQ=255)
                                                                          cAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGCCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCa                                                                                                                                          >  8:55689/1‑78 (MQ=255)
                                                                           aGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAg                                                                   >  5:305719/1‑148 (MQ=255)
                                                                             aTGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTaa                                                                <  8:287847/149‑1 (MQ=255)
                                                                               gAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATc                                                              >  8:237730/1‑149 (MQ=255)
                                                                                 gCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGa                                                                                                                         >  7:99529/1‑88 (MQ=255)
                                                                                 gCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGa                                                                                                                         <  8:99529/88‑1 (MQ=255)
                                                                                 gCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTg                                                            <  7:264402/149‑1 (MQ=255)
                                                                                 gCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGAGCCTGAACGTGGCGGCTAGTAATCTg                                                            >  6:198855/1‑149 (MQ=255)
                                                                                       gcgcGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGACAGTAATCTGGCGAAt                                                      >  8:131671/1‑149 (MQ=255)
                                                                                         gcgTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATAGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGc                                                    <  1:202419/149‑1 (MQ=255)
                                                                                         gcgTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATAGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGc                                                    <  3:144926/149‑1 (MQ=255)
                                                                                          cgTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCt                                                   <  1:253319/149‑1 (MQ=255)
                                                                                            ttGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATATTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGa                                                 <  7:327582/149‑1 (MQ=255)
                                                                                               aGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATa                                               <  4:152513/148‑1 (MQ=255)
                                                                                                gCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGc                                             <  6:61607/149‑1 (MQ=255)
                                                                                                  gggCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGt                                           >  4:239280/1‑149 (MQ=255)
                                                                                                    gCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTggcg                                                                        >  8:172727/1‑118 (MQ=255)
                                                                                                    gCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTggcg                                                                        <  7:172727/118‑1 (MQ=255)
                                                                                                     cAAATCACAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGAc                                        <  7:304411/149‑1 (MQ=255)
                                                                                                     cAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGAc                                        <  6:321711/149‑1 (MQ=255)
                                                                                                     cAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGAc                                        <  3:239280/149‑1 (MQ=255)
                                                                                                         tCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGt                                    >  1:108101/1‑149 (MQ=255)
                                                                                                          cAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTc                                   >  3:143932/1‑149 (MQ=255)
                                                                                                              ggCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGa                               <  7:75061/149‑1 (MQ=255)
                                                                                                              ggCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGa                               <  1:247899/149‑1 (MQ=255)
                                                                                                               gCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGAt                              <  4:264695/149‑1 (MQ=255)
                                                                                                                  tgatgaACGGTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGa                           <  6:56594/149‑1 (MQ=255)
                                                                                                                      gaACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGc                       >  5:216983/1‑149 (MQ=255)
                                                                                                                       aaCGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGc                       >  1:231238/1‑148 (MQ=255)
                                                                                                                       aaCGTGTTACAGAGCGGAAATTAAGGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGGCCCGATGGACAGcg                      <  6:69581/149‑2 (MQ=255)
                                                                                                                        aCGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCa                     <  5:52807/149‑1 (MQ=255)
                                                                                                                            tttACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATc                 >  2:257863/1‑149 (MQ=255)
                                                                                                                              tACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATTTCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCg                <  2:234750/148‑1 (MQ=255)
                                                                                                                               aCAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGa                                         <  8:298648/122‑1 (MQ=255)
                                                                                                                               aCAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCggg              >  3:181210/1‑149 (MQ=255)
                                                                                                                                cAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCggg              >  1:258410/1‑148 (MQ=255)
                                                                                                                                cAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCggg              <  5:266845/148‑1 (MQ=255)
                                                                                                                                cAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCAGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGACATATCggg              >  1:253880/1‑148 (MQ=255)
                                                                                                                                 agagCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGc             >  5:329127/1‑148 (MQ=255)
                                                                                                                                         aaTTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAggt    <  4:143932/149‑1 (MQ=255)
                                                                                                                                           ttAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAggtgg  <  6:216983/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                           
AGCCTTCGCTTGACGGTAATACCGTCGATATGGATCGCGAACGCACCCAGTTTGCCGATAACAGCCTGCAATACCAGATGAGCCTTAGCGCGTTGAGCGGGCAAATCAAAGGCATGATGAACGTTTTACAGAGCGGAAATTAACGGATGGCACTGCTGAATATTTTTGATATCGCCGGGTCGGCGTTAACTGCCCAGTCCCAGCGCCTGAACGTGGCGGCCAGTAATCTGGCGAATGCTGATAGCGTGACCGGTCCCGATGGACAGCCATATCGGGCAAAACAGGTGG  >  NC_000913/1131292‑1131579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: