Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,155,647 T→A 7.7% L175Q (CTG→CAG)  tmk → thymidylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,155,6470TA 7.7% 159.6 / 4.2 52L175Q (CTG→CAG) tmkthymidylate kinase
Reads supporting (aligned to +/- strand):  ref base T (22/26);  new base A (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

CTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGACCT  >  NC_000913/1155500‑1155788
                                                                                                                                                   |                                                                                                                                             
cTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCt                                                                                                                                               <  7:301632/148‑1 (MQ=255)
   ttCTCGGGGATTTTCGCCCCGACTTTACGCTCTATCTCGATGTTACCCCGGAAGTTTGCTTAAAACGCGCGCGCGCGCGCGGCGAGCTGGATCGTATTGAGCAATAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGac                                                                                                                                           <  1:70272/149‑2 (MQ=255)
   ttCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGaa                                                                                                                                           <  2:32810/149‑1 (MQ=255)
         gggATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTgg                                                                                                                                       >  6:31833/1‑147 (MQ=255)
         gggATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGgca                                                                                                                                     <  8:79307/149‑1 (MQ=255)
          ggATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGgcag                                                                                                                                    <  2:14483/149‑1 (MQ=255)
                 cGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAg                                                                                                                              <  6:203660/148‑1 (MQ=255)
                  gCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGa                                                                                                                             <  3:206224/148‑1 (MQ=255)
                      cGACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTACGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATaaaa                                                                                                                        <  3:150983/149‑1 (MQ=255)
                       gACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAg                                                                                                                       >  7:241813/1‑149 (MQ=255)
                            aaCGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCAtt                                                                                                                   <  5:143175/148‑1 (MQ=255)
                                         gATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGAt                                                                                                       <  8:264276/147‑1 (MQ=255)
                                         gATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATg                                                                                                      <  8:268579/148‑1 (MQ=255)
                                             ttACCCCTGAATTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGAACCCGCGCCCGCTATCTGTAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCAcc                                                                                                 <  4:38604/149‑1 (MQ=255)
                                                 cccGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGc                                                                                             >  7:158442/1‑149 (MQ=255)
                                                 cccGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGc                                                                                             >  5:229459/1‑149 (MQ=255)
                                                  ccGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGcc                                                                                            >  3:85942/1‑149 (MQ=255)
                                                   cGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCa                                                                                                                 <  3:144481/127‑1 (MQ=255)
                                                   cGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCa                                                                                                                 >  4:144481/1‑127 (MQ=255)
                                                            gCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATa                                                                                                               >  8:213504/1‑120 (MQ=255)
                                                            gCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATa                                                                                                               <  7:213504/120‑1 (MQ=255)
                                                             cTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATCCCATTGATGCCACCCAGCCGCTGGAGGCCg                                                                                 >  7:286942/1‑149 (MQ=255)
                                                              ttAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGt                                                                                >  5:135292/1‑149 (MQ=255)
                                                              ttAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGt                                                                                >  3:140897/1‑149 (MQ=255)
                                                              ttAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGt                                                                                >  7:148100/1‑149 (MQ=255)
                                                                   aCGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTgatgg                                                                           <  6:88317/149‑1 (MQ=255)
                                                                         gcgTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCaa                                                                     <  5:265774/149‑1 (MQ=255)
                                                                                        gCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGa                                                      <  8:148100/149‑1 (MQ=255)
                                                                                           ggATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGAccc                                                   >  8:238846/1‑149 (MQ=255)
                                                                                            gATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGAcc                                                    <  5:31833/147‑1 (MQ=255)
                                                                                            gATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCACACTACCGTGAcc                                                    <  6:319528/147‑1 (MQ=255)
                                                                                              tCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACt                                                >  1:179499/1‑149 (MQ=255)
                                                                                                gTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTgg                                              >  1:262168/1‑149 (MQ=255)
                                                                                                      aGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAg                                        >  6:116527/1‑149 (MQ=255)
                                                                                                      aGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAg                                        <  5:134199/149‑1 (MQ=255)
                                                                                                        cAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAg                                        >  3:85389/1‑147 (MQ=255)
                                                                                                         aaGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAg                                     >  2:299949/1‑149 (MQ=255)
                                                                                                         aaGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCAGGAACTGGCAGCACAAGATAAAAGc                                                                                                                      <  8:38763/68‑1 (MQ=255)
                                                                                                         aaGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCAGGAACTGGCAGCACAAGATAAAAGc                                                                                                                      <  8:33714/68‑1 (MQ=255)
                                                                                                         aaGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCAGGAACTGGCAGCACAAGATAAAAGc                                                                                                                      >  7:33714/1‑68 (MQ=255)
                                                                                                         aaGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCAGGAACTGGCAGCACAAGATAAAAGc                                                                                                                      >  7:38763/1‑68 (MQ=255)
                                                                                                          aGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGt                                    <  6:229459/149‑1 (MQ=255)
                                                                                                                tttCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGAc                               >  5:306005/1‑148 (MQ=255)
                                                                                                                 ttCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACg                              >  2:103318/1‑148 (MQ=255)
                                                                                                                  tCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGa                                <  2:179499/145‑1 (MQ=255)
                                                                                                                  tCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGAc                               <  6:306005/146‑1 (MQ=255)
                                                                                                                   cGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGTGTGAAGGAGTTGGACGCa                            <  4:85942/148‑1 (MQ=255)
                                                                                                                         cTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGAt                      <  7:238846/148‑1 (MQ=255)
                                                                                                                            tAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATg                     <  6:135292/146‑1 (MQ=255)
                                                                                                                               tCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATcc               <  8:158442/149‑1 (MQ=255)
                                                                                                                               tCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATc                >  1:326061/1‑148 (MQ=255)
                                                                                                                                    cccGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGt          >  4:148738/1‑149 (MQ=255)
                                                                                                                                             gCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCATCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACAGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGACCt  >  1:172346/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGAGATGGTATCCATGGTTACGACCT  >  NC_000913/1155500‑1155788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑