Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,976,324 C→T 6.9% S272S (TCG→TCA motA ← proton conductor component of flagella motor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,976,3240CT 6.9% 180.4 / 2.7 58S272S (TCG→TCAmotAproton conductor component of flagella motor
Reads supporting (aligned to +/- strand):  ref base C (26/28);  new base T (2/2);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

CCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGCATCATTTTGCTGGTTTCGGCGCTTTTCTGACGTAAAACAGTCGC  >  NC_000913/1976178‑1976470
                                                                                                                                                  |                                                                                                                                                  
ccACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAac                                                                                                                                                    <  8:265106/147‑1 (MQ=255)
    gATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACgacgga                                                                                                                                              >  4:114250/1‑149 (MQ=255)
                   ccGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGaa                                                                                                                                  >  7:77578/1‑146 (MQ=255)
                              ggctttGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGc                                                                                                                     <  7:249270/148‑1 (MQ=255)
                               gctttGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCg                                                                                                                    <  7:58283/148‑1 (MQ=255)
                               gctttGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGa                                                                                                                   >  6:135449/1‑149 (MQ=255)
                                   tGCTTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCa                                                                                                                <  4:46235/148‑1 (MQ=255)
                                    gCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGAACaaa                                                                                                              >  5:169246/1‑149 (MQ=255)
                                     cgtcgtTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAAc                                                                                                             >  2:247169/1‑149 (MQ=255)
                                          tttGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAAc                                                                                                        <  2:291079/149‑1 (MQ=255)
                                             gacgacAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACgg                                                                                                      <  8:89746/148‑1 (MQ=255)
                                                gacAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCTCTGGAATAGAGCGTTTAGCGACCAAACTCAACGGCGa                                                                                                   >  5:158428/1‑148 (MQ=255)
                                                gacAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGa                                                                                                   >  7:132993/1‑148 (MQ=255)
                                                gacAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGAt                                                                                                  <  5:31849/149‑1 (MQ=255)
                                                   aataatCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCaaa                                                                                                              >  2:8261/1‑134 (MQ=255)
                                                   aataatCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCaaa                                                                                                              <  1:8261/134‑1 (MQ=255)
                                                    ataatCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGc                                                                                              <  5:271223/149‑1 (MQ=255)
                                                      aatCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCgg                                                                                            >  3:182769/1‑149 (MQ=255)
                                                       atCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGt                                                                                           <  2:213146/149‑1 (MQ=255)
                                                        tCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACTGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTg                                                                                          >  7:55833/1‑149 (MQ=255)
                                                        tCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTg                                                                                          <  5:135449/149‑1 (MQ=255)
                                                           gATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCg                                                                                        >  7:303016/1‑148 (MQ=255)
                                                                gcTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCg                                                                                  >  8:272521/1‑149 (MQ=255)
                                                                 cTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGt                                                                                 >  8:214955/1‑149 (MQ=255)
                                                                  ttGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGATGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGtt                                                                                >  7:34718/1‑149 (MQ=255)
                                                                     aTTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCa                                                                              <  1:247169/148‑1 (MQ=255)
                                                                        cttcATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACTCACATGCCCTTCCAGGTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGAt                                                                           <  2:308814/148‑1 (MQ=255)
                                                                        cttcATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGAt                                                                           >  2:85282/1‑148 (MQ=255)
                                                                               cTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGc                                                                   <  7:214955/149‑1 (MQ=255)
                                                                                   cTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCagag                                                               <  8:303016/149‑1 (MQ=255)
                                                                                   cTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCagag                                                               <  8:263964/149‑1 (MQ=255)
                                                                                   cTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCaga                                                                <  8:132993/148‑1 (MQ=255)
                                                                                    tCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGaa                                                                                                                                  <  2:279134/81‑1 (MQ=255)
                                                                                    tCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGaa                                                                                                                                  >  1:279134/1‑81 (MQ=255)
                                                                                     cGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGt                                                              >  8:193/1‑148 (MQ=255)
                                                                                      ggTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGt                                                              <  8:204396/147‑1 (MQ=255)
                                                                                          gtcgtcTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAATGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTc                                                                                                           <  8:39746/98‑1 (MQ=255)
                                                                                          gtcgtcTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAATGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTc                                                                                                           <  8:44887/98‑1 (MQ=255)
                                                                                          gtcgtcTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAATGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTc                                                                                                           >  7:39746/1‑98 (MQ=255)
                                                                                          gtcgtcTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAATGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTc                                                                                                           >  7:44887/1‑98 (MQ=255)
                                                                                              tcTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCACCGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACg                                                    >  4:187541/1‑149 (MQ=255)
                                                                                                 gttgttGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCAc                                                 >  4:281762/1‑149 (MQ=255)
                                                                                                  ttgttgCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCa                                                  <  4:287434/147‑1 (MQ=255)
                                                                                                  ttgttgCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCAc                                                 <  3:310951/148‑1 (MQ=255)
                                                                                                  ttgttgCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCAc                                                 <  1:85282/148‑1 (MQ=255)
                                                                                                       gCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGcat                                            >  1:115875/1‑148 (MQ=255)
                                                                                                        cGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGcatca                                          >  8:85318/1‑149 (MQ=255)
                                                                                                          gATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGTACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGCATCAtt                                        <  4:268743/149‑1 (MQ=255)
                                                                                                          gATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGTCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGCATCAtt                                        >  2:147308/1‑149 (MQ=255)
                                                                                                                          cacaTGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGCATCATTTTGCTGGTTTCGgcgc                        <  6:158428/149‑1 (MQ=255)
                                                                                                                              tGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGCATCATTTTGCTGGTTTCGGCGCtttt                    <  7:193/149‑1 (MQ=255)
                                                                                                                                     ccAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGCATCATTTTGCTGGTTTCGGCGCTTTTCTGAc               <  8:77578/147‑1 (MQ=255)
                                                                                                                                      cAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGCATCATTTTGCt                                   >  1:304181/1‑126 (MQ=255)
                                                                                                                                      cAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGCATCATTTTGCt                                   <  2:304181/126‑1 (MQ=255)
                                                                                                                                         ttCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGCATCATTTTGCTGGTTTCGGCGCTTTTCTGACGTaaaa         >  7:269143/1‑149 (MQ=255)
                                                                                                                                            aaTAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGCATCATTTTGCTGGTTTCGGCGCTTTTCTGACGTAAAACAg      >  7:284965/1‑149 (MQ=255)
                                                                                                                                              tAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGCATCATTTTGCTGGTTTCGGCGCTTTTCTGACGTAAAACAGTc    >  8:286354/1‑149 (MQ=255)
                                                                                                                                                aaCGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGCATCATTTTGCTGGTTTCGGCGCTTTTCTGACGTAAAACAGTCg   <  7:85318/148‑1 (MQ=255)
                                                                                                                                                aaCGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGCATCATTTTGCTGGTTTCGGCGCTTTTCTGACGTAAAACAGTCGc  <  3:114250/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCACGATCCATGTGCTGCCCCGTGGCTTTTGGCTTTGCGTCGTTTGACGACAATAATCGGATGCGCTTGATTCTTCATGCTTCCTCGGTTGTCGTCTGTTGTTGCGGATTTTTCACCGCACGCACATGCTCTTCCAGTTCAATAAACGACGGACGTTCGCTGGAATAGAGCGTTTTGCGACCAAACTCAACGGCGATAGGCGGTGCGTAACCGTTCAGATTAGAAAGCAGAGTGACTTTGACGCACTGCATCATTTTGCTGGTTTCGGCGCTTTTCTGACGTAAAACAGTCGC  >  NC_000913/1976178‑1976470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: