Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,982,585 C→A 6.8% R319L (CGT→CTT)  araH ← L‑arabinose ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,982,5850CA 6.8% 193.1 / 3.5 59R319L (CGT→CTT) araHL‑arabinose ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (25/30);  new base A (2/2);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

GACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTT  >  NC_000913/1982440‑1982733
                                                                                                                                                  |                                                                                                                                                    
gagaTGTAAGTATTGACAGTGTGACGGGGGGCGGCATTTGCAACGAAGAACAGCCAACGGTTATGGCGGTTGGCTATGGTGGGAAAAAAGGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGt                                                                                                                                                   <  6:104887/146‑1 (MQ=255)
  cATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGTAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAATTTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCg                                                                                                                                                 <  8:275742/149‑1 (MQ=255)
     gTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCGAAATTGTTGCAGCAAAAAGCATCAGCCAGTGCGTTTCGCTTTTTGCTTGTAACGGGCg                                                                                                                                                 >  4:219899/1‑146 (MQ=255)
      tAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCg                                                                                                                                                 >  7:189156/1‑145 (MQ=255)
          tataGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCAc                                                                                                                                         <  1:248137/149‑1 (MQ=255)
            taGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCAcc                                                                                                                                        <  7:38908/148‑1 (MQ=255)
            taGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCg                                                                                                                                       >  7:278764/1‑149 (MQ=255)
                 aGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGc                                                                                                                                   >  3:306583/1‑148 (MQ=255)
                  gtgtGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGc                                                                                                                                   >  8:66822/1‑147 (MQ=255)
                       aCGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCcagcag                                                                                                                             >  7:220518/1‑148 (MQ=255)
                         gggggTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGAt                                                                                                                          <  7:113745/149‑1 (MQ=255)
                         gggggTCGCCATTTGAAAATAAAAAAAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAGAAACGCGATATTGGTGCAGCAAACAGCATCGGCCGGTGCGTTTGGCTGTTTGCTTGTATCGGTCGCAGATCAGCGCTGCCAGCAGGAt                                                                                                                          >  6:182321/1‑149 (MQ=255)
                           gggTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGAtt                                                                                                                         >  4:186016/1‑148 (MQ=255)
                                gCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTa                                                                                                                        >  7:48196/1‑144 (MQ=255)
                                gCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTa                                                                                                                        <  8:48196/144‑1 (MQ=255)
                                       gCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCcagca                                                                                                                              >  6:11252/1‑131 (MQ=255)
                                       gCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCcagca                                                                                                                              <  5:11252/131‑1 (MQ=255)
                                        cAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGGGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGAGTCAGCCGCGAACCAc                                                                                                           >  6:77462/1‑149 (MQ=255)
                                         aaCGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCcagc                                                                                                                               <  7:62177/128‑1 (MQ=255)
                                         aaCGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCcagc                                                                                                                               >  8:62177/1‑128 (MQ=255)
                                         aaCGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACg                                                                                                          >  2:41775/1‑149 (MQ=255)
                                                     ccAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGTCGCGAACCACGTACTGCGCGaaa                                                                                              <  1:284796/149‑1 (MQ=255)
                                                     ccAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGaaa                                                                                              <  5:131290/149‑1 (MQ=255)
                                                      cAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAg                                                                                             <  3:186016/149‑1 (MQ=255)
                                                       aaCCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAg                                                                                             >  2:304951/1‑148 (MQ=255)
                                                       aaCCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAg                                                                                             >  3:250542/1‑148 (MQ=255)
                                                            ttATGGCGGGTGGCTATGGTGGGAAAAAACGCGAATTTGTTGCAGAAAAAAGCTTCAGACAGTGCGTTTCGCTTTTTCCTTGTAACGGACGAAGATAACCCCTACCACAAGGATTAAGCCGCGAACAACGAACTGCGCGAAAgaagaa                                                                                        >  1:239647/1‑148 (MQ=255)
                                                                 gCGGTTGGCTATTGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATAtt                                                                                   <  4:238955/149‑1 (MQ=255)
                                                                       ggCTATGGTTGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTTAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAg                                                                             <  6:277774/148‑1 (MQ=255)
                                                                          tATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGtt                                                                          >  6:116976/1‑148 (MQ=255)
                                                                          tATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAAAGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGtt                                                                          >  2:27493/1‑148 (MQ=255)
                                                                          tATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAAAGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGtt                                                                          >  4:287921/1‑148 (MQ=255)
                                                                            tggtggGAAAATACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACGACGTACTGCGGGAAAGGAGAAATATTAAGCAGGTTCa                                                                        <  2:239647/148‑1 (MQ=255)
                                                                              gtggGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATg                                                                      <  8:220518/148‑1 (MQ=255)
                                                                                 ggAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGt                                                                  >  7:8438/1‑149 (MQ=255)
                                                                                   aaaaaaCGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGttt                                                                >  5:51514/1‑149 (MQ=255)
                                                                                   aaaaaaCGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACATACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGttt                                                                <  4:306583/149‑1 (MQ=255)
                                                                                     aaaaCGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTc                                                              <  4:16675/149‑1 (MQ=255)
                                                                                     aaaaCGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTc                                                              <  6:200418/149‑1 (MQ=255)
                                                                                       aaCGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCTCCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCa                                                            <  1:6894/149‑1 (MQ=255)
                                                                                          gCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACgg                                                         >  1:140042/1‑149 (MQ=255)
                                                                                              aaTTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGc                                                      <  8:278764/148‑1 (MQ=255)
                                                                                                     gCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAAt                                               >  7:256193/1‑148 (MQ=255)
                                                                                                      cAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAAtt                                              >  6:281450/1‑148 (MQ=255)
                                                                                                       aGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAAtt                                              >  6:110300/1‑147 (MQ=255)
                                                                                                       aGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTaa                                            <  3:219899/149‑1 (MQ=255)
                                                                                                       aGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTaa                                            <  2:140042/149‑1 (MQ=255)
                                                                                                       aGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAAAGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTaa                                            <  3:287921/149‑1 (MQ=255)
                                                                                                       aGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAAAGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTaa                                            <  1:27493/149‑1 (MQ=255)
                                                                                                        gAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAg                                           <  6:51514/149‑1 (MQ=255)
                                                                                                            aaaaGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATa                                        <  5:182321/149‑1 (MQ=255)
                                                                                                               aGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATAccc                                     <  8:270760/149‑1 (MQ=255)
                                                                                                                      gACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGccacca                              <  1:41775/149‑1 (MQ=255)
                                                                                                                      gACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTGCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGccacca                              <  4:250542/149‑1 (MQ=255)
                                                                                                                         aGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAg                                                                             >  4:189971/1‑99 (MQ=255)
                                                                                                                         aGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAg                                                                             <  3:189971/99‑1 (MQ=255)
                                                                                                                           tGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGccac                                <  4:269634/142‑1 (MQ=255)
                                                                                                                           tGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGccac                                >  3:269634/1‑142 (MQ=255)
                                                                                                                                       tttGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTc               <  7:66822/147‑1 (MQ=255)
                                                                                                                                                  cGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCTCCACCACATATGAGATTTTTCCGATGCCACCttt  <  1:304951/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GACATGTAAGTATAGACAGTGTGACGGGGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATGCCACCTTT  >  NC_000913/1982440‑1982733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: