Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 340,050 G→T 7.1% intergenic (+307/‑115) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913340,0500GT 7.1% 194.2 / 2.1 68intergenic (+307/‑115)yahG/yahIDUF1116 family protein/carbamate kinase‑like protein
Reads supporting (aligned to +/- strand):  ref base G (27/35);  new base T (2/4);  total (29/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTG  >  NC_000913/339907‑340195
                                                                                                                                               |                                                                                                                                                 
cTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGc                                                                                                                                              >  3:14979/1‑149 (MQ=255)
 tGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGcc                                                                                                                                             >  1:88803/1‑149 (MQ=255)
 tGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGcc                                                                                                                                             >  5:204886/1‑149 (MQ=255)
 tGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGAATTTGTCACCATTGCCGGATGCGGCGTGAACGcc                                                                                                                                             >  7:156737/1‑149 (MQ=255)
   attaGCGTATCGGATCAGGCATTTGCCCCCACTGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCtt                                                                                                                                           <  8:505/146‑1 (MQ=14)
      aGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATAAGGCGTTCACGccgcatc                                                                                                                                        <  8:97534/149‑6 (MQ=14)
         gTAGCGCATCAGGCATTAGCCACAACTGCCTTATCCCAACAGCACCCCTTAACCGGACTACAACTAGCTCGAGAAACTGTAGGCCTGATAAGCGTATCGAATCAGGAATTTATCAACATTGCCGGATGCTGCCAGAAATCATTATCCgg                                                                                                                                     >  4:46763/1‑149 (MQ=9)
            gcgcATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                   <  4:163333/148‑1 (MQ=255)
            gcgcATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                   <  8:151524/148‑1 (MQ=255)
                aTCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                    <  6:181072/143‑1 (MQ=255)
                aTCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                    >  5:181072/1‑143 (MQ=255)
                aTCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGa                                                                                                                              <  1:247004/149‑1 (MQ=255)
                aTCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGa                                                                                                                              <  1:140385/149‑1 (MQ=255)
                             caccacTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAt                                                                                                                 >  6:300669/1‑149 (MQ=255)
                              accacTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAtt                                                                                                                >  5:14699/1‑149 (MQ=255)
                                cacTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTg                                                                                                               >  4:130975/1‑148 (MQ=255)
                                 acTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTa                                                                                                             <  4:44179/149‑1 (MQ=255)
                                  cTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAg                                                                                                            <  7:259439/149‑1 (MQ=255)
                                     ccGTATGCGGCGTGTACGCCTTATCCGGCCTACGAGTGTCGCTAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGc                                                                                                          <  5:310279/148‑1 (MQ=255)
                                     ccGGATGCGGCGTTTACGCCTTATCCGGCCTACGAGTGGCGCTAGAATCTTTTGGCCTGTTAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGc                                                                                                          <  4:169131/148‑1 (MQ=14)
                                        gATGCGTCGTGTACGCCTTCTCCGGCCTACGACTGGCGCTAGAATCTGTAGGCCTGCTAAGCGTAGCGCATCAGGCATTTGTCTCCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGa                                                                                                      <  4:60912/149‑1 (MQ=11)
                                            cggcgTGGACGCCCTAGCCGGCCTACGATGGGCGCGAGACTCTGTCGGCCTGATAAGCGGAGCGCATCAGGCATTTGTCCCCATTGCCGCCGGCGGCGTGAACGCCTTATCCGGCCTACAATTCTCGCGCCACTCGTAGGCCGGATaa                                                                                                   <  6:244884/148‑1 (MQ=2)
                                                 gggACGCCTTAGCCGGCCTCCGAGTGGTGTTTGTATCTGTTGGCCGGCTCAGCGTAGCGCTTCAGGCTTTTGTCACCATTGCTGGATGCGGCGTGACCGCCTTATCGGGCCTAGGAGGGGGGCGTTATTTGTAGGCCTGATAAGCGTAg                                                                                             <  2:102440/148‑1 (MQ=9)
                                                         ttATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGcagcag                                                                                     >  3:173176/1‑149 (MQ=255)
                                                         ttATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAg                                                                                     >  6:120356/1‑149 (MQ=255)
                                                             ccGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAtt                                                                                                                >  6:202303/1‑118 (MQ=255)
                                                             ccGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAtt                                                                                                                <  5:202303/118‑1 (MQ=255)
                                                               ggCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTc                                                                               >  2:296398/1‑149 (MQ=255)
                                                                gCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGCTGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCt                                                                              <  5:300234/149‑1 (MQ=255)
                                                                 ccTACGAGTGGCGCGAGCATCTGTAGGCCGGATACGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAGGCGTAGCGCATCAGGCAGTCTg                                                                             <  3:4312/149‑1 (MQ=255)
                                                                       aGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTg                                                                       <  3:130975/149‑1 (MQ=255)
                                                                       aGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTg                                                                       <  6:14699/149‑1 (MQ=255)
                                                                          ggCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTc                                                                    <  3:125954/149‑1 (MQ=255)
                                                                           tcgctAGAATCTGTAGGCCTGATAAGAGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGATCGCCTTATCCGGCCTACGAGTGGCGCGATAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCa                                                                   <  2:117397/148‑1 (MQ=255)
                                                                           gcgcttgaATCTGTTGGCCTGATAAGCGTAGCGCATCAGGCATTTTTCACCATTTCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGGGGGGCTTTATTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCa                                                                   <  8:326098/143‑1 (MQ=17)
                                                                              ctttaaTCTGTATGCCTGATAAGCGTATCGCATCAGTAATTTTTCACCATTGCCGTATGCGTCGTGAACGCCTTATCCGGCATACTATTGGCGCGATAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATaa                                                                <  2:129105/145‑1 (MQ=17)
                                                                                 gaATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAAccc                                                             >  1:74982/1‑149 (MQ=255)
                                                                                   aTCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa                                                           <  2:88803/149‑1 (MQ=255)
                                                                                      tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCa                                                        >  2:35665/1‑149 (MQ=255)
                                                                                          ggCCTGATAAGCGTAGCGCATCAGGCTTTTGTCACCATTGCCGGATGCGGCGTGAACGCATTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCct                                                    <  5:171061/149‑1 (MQ=255)
                                                                                          ggCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAAc                                                               <  2:230126/138‑1 (MQ=255)
                                                                                          ggCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAAc                                                               >  1:230126/1‑138 (MQ=255)
                                                                                                aTAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAg                                              >  2:297760/1‑149 (MQ=255)
                                                                                                    gCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCGCCCTCTGTAGCGGa                                          >  2:182299/1‑149 (MQ=255)
                                                                                                       tAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACAt                                       <  6:142663/149‑1 (MQ=255)
                                                                                                         gcgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                     <  1:296398/149‑1 (MQ=255)
                                                                                                            cATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaacaa                                  >  4:153826/1‑149 (MQ=255)
                                                                                                             aTCAGGCATTTGTCTCCATTGCCGTATGCGGCGTTAAGTCCTTATCTGGCCTAAGTGTGGCGCGATAATTTTAGCCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaacaac                                 <  3:46763/149‑1 (MQ=25)
                                                                                                             aTCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaacaa                                  >  7:34420/1‑148 (MQ=255)
                                                                                                             aTCAGGCATTTGTCAACATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaacaa                                  >  7:45001/1‑148 (MQ=255)
                                                                                                                aGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACAt                               >  3:129373/1‑148 (MQ=255)
                                                                                                                 ggCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATg                              >  7:166112/1‑148 (MQ=255)
                                                                                                                  gCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGa                             >  4:301525/1‑148 (MQ=255)
                                                                                                                   cATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGaaa                           <  2:74982/149‑1 (MQ=255)
                                                                                                                   cATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGaaa                           <  8:156737/149‑1 (MQ=255)
                                                                                                                   cATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGaaa                           <  6:204886/149‑1 (MQ=255)
                                                                                                                   cATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGaaa                           <  4:291498/149‑1 (MQ=255)
                                                                                                                            ccATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTtgtg                  <  5:120356/149‑1 (MQ=255)
                                                                                                                             cATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTgg                 >  1:226756/1‑149 (MQ=255)
                                                                                                                               ttGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATGACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTc               >  3:279370/1‑149 (MQ=255)
                                                                                                                                 gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATaa                                                                <  1:232000/98‑1 (MQ=18)
                                                                                                                                 gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATaa                                                                >  2:232000/1‑98 (MQ=18)
                                                                                                                                 gCCGGATAAGGCGGTCACGCCGCATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGCTCAGCGGAGCGCATCAGGCAGTCTGGCGTTGGTCATaa                                                                <  1:236603/98‑1 (MQ=18)
                                                                                                                                  ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGtt            <  1:35665/149‑1 (MQ=255)
                                                                                                                                    ggATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGc          >  6:299599/1‑149 (MQ=255)
                                                                                                                                      ataaggcgTTCACGCCGCATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCa        >  8:5783/5‑149 (MQ=37)
                                                                                                                                       tGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGTGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCAt       <  1:277368/149‑1 (MQ=255)
                                                                                                                                            cgTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATtggtg  <  4:14979/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTG  >  NC_000913/339907‑340195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: