Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,388,885 C→A 5.6% P84H (CCC→CAC)  yfbO → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,388,8850CA 5.6% 228.2 / 3.6 72P84H (CCC→CAC) yfbOuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (30/38);  new base A (2/2);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01

ACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCAT  >  NC_000913/2388738‑2389033
                                                                                                                                                   |                                                                                                                                                    
aCAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGccc                                                                                                                                                     >  8:217998/1‑149 (MQ=255)
aCAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGccc                                                                                                                                                     >  2:105613/1‑149 (MQ=255)
aCAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGccc                                                                                                                                                     <  1:289278/149‑1 (MQ=255)
    taataTCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTa                                                                                                                                                 <  3:160946/149‑1 (MQ=255)
       tatCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGt                                                                                                                                               >  8:164992/1‑148 (MQ=255)
           cATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGaaa                                                                                                                                           <  6:162660/148‑1 (MQ=255)
           cATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGaaa                                                                                                                                           <  1:271615/148‑1 (MQ=255)
           cATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAg                                                                                                                                          >  6:187855/1‑149 (MQ=255)
           cATGGTTCAATATGTTGCAATGTGATTCCAGAAAAATCTCAGAAAGATATCTATCACCATTTTCTGAAAATACGTGATCGTAACAATGTTATTGATGTTTTGGTAGAAATTACTATGTTCGATGACCCAGACTGGCCATTTAGTGAAAg                                                                                                                                          >  5:112843/1‑149 (MQ=255)
                ttCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTAttt                                                                                                                                     <  8:300589/149‑1 (MQ=255)
                   aaTATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTa                                                                                                                                   <  3:234929/148‑1 (MQ=255)
                     tatGTTGGAATGTGATTCAAGAACTATCTCCGCAATCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGtt                                                                                                                                <  3:285422/149‑1 (MQ=255)
                        gTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTAt                                                                                                                              >  6:56048/1‑148 (MQ=255)
                         ttGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATc                                                                                                                             <  5:56048/148‑1 (MQ=255)
                              aTGTGATTCCAGAACAATATCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTAGATGAACCATACTGGCCCTTTAGTGAAAGTATTTTAGATATCACCACt                                                                                                                       >  1:52374/1‑149 (MQ=255)
                                      ccAGAACAATCTCCGAAAGCTATCTATCACCATTTTCTGAAAATACGTGAGAGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCAc                                                                                                                <  6:112843/148‑1 (MQ=255)
                                       cAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGCCCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCAc                                                                                                                >  2:68981/1‑147 (MQ=255)
                                       cAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCAcc                                                                                                               <  2:288674/148‑1 (MQ=255)
                                        aGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCAc                                                                                                                >  5:286931/1‑146 (MQ=255)
                                        aGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCAc                                                                                                                >  4:209022/1‑146 (MQ=255)
                                          aaCAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCag                                                                                                             >  1:49636/1‑147 (MQ=255)
                                          aaCAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCag                                                                                                             >  3:24343/1‑147 (MQ=255)
                                              aTCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGaagaag                                                                                                       >  4:23026/1‑149 (MQ=255)
                                                 tcCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGtt                                                                                                     >  6:61494/1‑148 (MQ=255)
                                                   cGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGtt                                                                                                     <  2:124628/146‑1 (MQ=255)
                                                   cGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGtt                                                                                                     >  5:299514/1‑146 (MQ=255)
                                                      aaGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGc                                                                                               <  8:327477/149‑1 (MQ=255)
                                                                tcACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGttt                                                                                         <  7:217998/145‑1 (MQ=255)
                                                                  aCCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGttt                                                                                         >  2:221610/1‑143 (MQ=255)
                                                                  aCCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGttt                                                                                         <  1:221610/143‑1 (MQ=255)
                                                                  aCCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGt                                                                                           <  4:117451/141‑1 (MQ=255)
                                                                  aCCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGt                                                                                           >  3:117451/1‑141 (MQ=255)
                                                                  aCCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTag                                                                                     >  6:64072/1‑147 (MQ=255)
                                                                  aCCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCACTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTag                                                                                     >  4:114907/1‑147 (MQ=255)
                                                                  aCCATTTACAGAAAATACGTGAGCGTAACAATGATAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCACTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTagaa                                                                                   >  4:110400/1‑149 (MQ=255)
                                                                    cATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGGTTTGGTAGAAATTACGATGTTCGATGCCCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTagaaga                                                                                 <  8:309299/149‑1 (MQ=255)
                                                                         tCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAAtt                                                                             <  4:30010/148‑1 (MQ=255)
                                                                          cTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTg                                                                            <  5:162578/148‑1 (MQ=255)
                                                                             aaaaTACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGcccc                                                                        <  2:49636/149‑1 (MQ=255)
                                                                             aaaaTACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGccc                                                                         <  7:78484/148‑1 (MQ=255)
                                                                             aaaaTACGTGAGCCTAACAATGGTAGTGATGTTTAGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGACAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGTAGAAATTGc                                                                           <  6:22428/146‑1 (MQ=255)
                                                                                aTACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGATATTGCCCCtga                                                                     <  3:23026/149‑1 (MQ=255)
                                                                                    gTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAg                                                                 >  8:123522/1‑149 (MQ=255)
                                                                                          gTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTgg                                                           <  4:24343/149‑1 (MQ=255)
                                                                                             aCAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGaa                                                        <  1:105613/149‑1 (MQ=255)
                                                                                             aCAATGTTAGTGATGTTTTGGTAGAAATTACGATGTGCGATGAACCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGaa                                                        <  4:98255/149‑1 (MQ=255)
                                                                                               aaTGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAgg                                                      <  7:164992/149‑1 (MQ=255)
                                                                                               aaTGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTAGGAAg                                                       >  7:168139/1‑148 (MQ=255)
                                                                                                     aGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGt                                                 <  8:126926/148‑1 (MQ=255)
                                                                                                      gTGATGTGTTTGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTCGTGTAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTc                                                <  2:254016/148‑1 (MQ=255)
                                                                                                       tGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGt                                                 >  1:229243/1‑146 (MQ=255)
                                                                                                       tGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGt                                                 >  1:196984/1‑146 (MQ=255)
                                                                                                             tttGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGCAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATa                                        >  3:277244/1‑149 (MQ=255)
                                                                                                                ggTAGAAATTACGATGTTCGATGACCCAGACTGGCACTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACg                                      <  3:114907/148‑1 (MQ=255)
                                                                                                                ggTAGAAATTACGATGTTCGATGACCCAGACTGGCACTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACg                                      <  3:110400/148‑1 (MQ=255)
                                                                                                                 gTAGAAATTACGATGTTAGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTAAGAGCTGGTTTGTAGTAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGa                                    <  5:26740/149‑1 (MQ=255)
                                                                                                                    gAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCACTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCa                                 >  1:59602/1‑149 (MQ=255)
                                                                                                                        ttACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCCCCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCatgga                             <  6:214486/149‑1 (MQ=255)
                                                                                                                        ttACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTAGGAAGGATGGTCAGAAGATACGGAGCatgga                             <  8:168139/149‑1 (MQ=255)
                                                                                                                            gATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATggg                         <  2:229243/149‑1 (MQ=255)
                                                                                                                                 tCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTg                      >  1:143616/1‑147 (MQ=255)
                                                                                                                                  cGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGACGAACTTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGaa                    <  5:187855/148‑1 (MQ=255)
                                                                                                                                       aCCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCt              >  8:322800/1‑149 (MQ=255)
                                                                                                                                        cccAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCtg             >  5:282728/1‑149 (MQ=255)
                                                                                                                                         ccAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGGTGGTGTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCtgt            <  2:52374/149‑1 (MQ=255)
                                                                                                                                         ccAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCtgt            >  3:201341/1‑149 (MQ=255)
                                                                                                                                         ccAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCtgt            >  3:183477/1‑149 (MQ=255)
                                                                                                                                           aGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGa                                    <  7:116950/123‑1 (MQ=255)
                                                                                                                                           aGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGa                                    >  8:116950/1‑123 (MQ=255)
                                                                                                                                             aCTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGt        <  7:123522/149‑1 (MQ=255)
                                                                                                                                             aCTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGt        <  1:195440/149‑1 (MQ=255)
                                                                                                                                               tGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTAt      >  5:77741/1‑149 (MQ=255)
                                                                                                                                                 gCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTAt      <  2:196984/147‑1 (MQ=255)
                                                                                                                                                 gCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTAt      <  1:194335/147‑1 (MQ=255)
                                                                                                                                                 gCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATg     >  8:85530/1‑148 (MQ=255)
                                                                                                                                                   ccTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTTAAACTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGTAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCAt  >  1:282042/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGCAT  >  NC_000913/2388738‑2389033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: