Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,636,442 C→T 5.8% L305L (CTG→CTA der ← GTPase; multicopy suppressor of ftsJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,636,4420CT 5.8% 219.8 / 2.7 69L305L (CTG→CTAderGTPase; multicopy suppressor of ftsJ
Reads supporting (aligned to +/- strand):  ref base C (32/33);  new base T (2/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

CAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGT  >  NC_000913/2636294‑2636588
                                                                                                                                                    |                                                                                                                                                  
cAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCcagc                                                                                                                                                    <  8:121415/149‑1 (MQ=255)
cAGAGATAAAGTGCACACGAGCAAAATCGAGAAAGCCCAGACGGAAGTCGATCCTTTCTATCACCTGCTATTACACTTCCTGACTCAGGCCAGCCCACGGATTCCCCCCAATGACAAGCCAGCGCCCACTATAGAGAATACAGCCcagc                                                                                                                                                    >  5:285287/1‑149 (MQ=255)
     aTAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCga                                                                                                                                               <  1:284191/149‑1 (MQ=255)
      tAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGTGAATAAAGCCCAGCAGCgag                                                                                                                                              <  2:169140/149‑1 (MQ=255)
      tAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCgag                                                                                                                                              <  3:296888/149‑1 (MQ=255)
               cacGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGa                                                                                                                                     <  2:1749/149‑1 (MQ=255)
                      aaaaTCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGaaa                                                                                                                              <  8:304917/149‑1 (MQ=255)
                      aaaaTCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGaaa                                                                                                                              >  3:240421/1‑149 (MQ=255)
                      aaaaTCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGaaa                                                                                                                              <  3:179601/149‑1 (MQ=255)
                       aaaTCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAAt                                                                                                                             <  8:55115/149‑1 (MQ=255)
                         aTCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATAc                                                                                                                           >  1:38777/1‑149 (MQ=255)
                          tCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGTAGCGAGAGATCCTGATCGGAAATAcc                                                                                                                          >  3:48963/1‑149 (MQ=255)
                          tCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGTAGCGAGAGATCCTGATCGGAAATAcc                                                                                                                          >  3:44735/1‑149 (MQ=255)
                             aTAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTc                                                                                                                       <  8:8712/149‑1 (MQ=255)
                                aaGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTcgc                                                                                                                     <  1:95404/148‑1 (MQ=255)
                                    ccAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCa                                                                                                                <  4:157288/149‑1 (MQ=255)
                                    ccAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCa                                                                                                                >  1:277228/1‑149 (MQ=255)
                                       gACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAAGAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGAGCGCagc                                                                                                              >  6:248026/1‑146 (MQ=255)
                                           gAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCatcaatca                                                                                                         <  2:277228/149‑1 (MQ=255)
                                           gAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCatcaatca                                                                                                         >  3:53484/1‑149 (MQ=255)
                                               tCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTaa                                                                                                     <  1:315825/149‑1 (MQ=255)
                                                  aGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACAt                                                                                                  >  8:310573/1‑149 (MQ=255)
                                                      tttctttcACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAACGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATcac                                                                                               >  5:326013/1‑148 (MQ=255)
                                                          tttcACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGt                                                                                          >  7:220374/1‑149 (MQ=255)
                                                            tcACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGtt                                                                                         <  3:2530/148‑1 (MQ=255)
                                                             cACCTTCTGTTTCACTTACGGTCTCAGGCCATCCCACTTTTTCACCACAATGACAAGTCTGGGCCCACTATTGAGAATCTAGCCCCGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCTCATCAATCACTAACATCACCACGTTg                                                                                        <  6:285287/148‑1 (MQ=255)
                                                                cTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGt                                                                                    >  7:216293/1‑149 (MQ=255)
                                                                cTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGt                                                                                    <  2:340753/149‑1 (MQ=255)
                                                                cTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGt                                                                                    <  4:240421/149‑1 (MQ=255)
                                                                 tGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTc                                                                                   >  7:305701/1‑149 (MQ=255)
                                                                        tCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAAt                                                                             <  6:137515/148‑1 (MQ=255)
                                                                            ttCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCt                                                                        >  1:21653/1‑149 (MQ=255)
                                                                            ttCCTGACTCAGGCCATCCAACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCt                                                                        >  8:123477/1‑149 (MQ=255)
                                                                              ccTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCgagag                                                                                                                                            >  6:260101/1‑79 (MQ=255)
                                                                              ccTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCgagag                                                                                                                                            <  5:260101/79‑1 (MQ=255)
                                                                                  aCTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGAACCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACg                                                                  <  6:123476/149‑1 (MQ=255)
                                                                                    taaGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGtt                                                                >  8:180735/3‑149 (MQ=255)
                                                                                    tCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATTGCCTGCAACGtt                                                                >  1:254084/1‑149 (MQ=255)
                                                                                    tCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGtt                                                                >  6:990/1‑149 (MQ=255)
                                                                                      aGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGtt                                                                <  1:206871/147‑1 (MQ=255)
                                                                                        gCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGa                                                            >  6:110141/1‑149 (MQ=255)
                                                                                             cccACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACg                                                       >  3:172192/1‑149 (MQ=255)
                                                                                              ccACTTATTCACCACAATGACAAGTGCGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCCCCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACg                                                       >  2:88152/1‑148 (MQ=255)
                                                                                                aCTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGTAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCGCCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGgag                                                    <  4:44735/149‑1 (MQ=255)
                                                                                                aCTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGTAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCGCCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGgag                                                    <  4:48963/149‑1 (MQ=255)
                                                                                                 cTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGgag                                                    >  1:240470/1‑148 (MQ=255)
                                                                                                  ttATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGaa                                                  >  6:36190/1‑149 (MQ=255)
                                                                                                  ttATTCACCACAATCACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGACACATCCTGATCGGAAATACCTACGCGCGCATCAATCACTAACATCACCACGTTGCCGACTACAATGGCCTGCAACGTTTTGATTACGGAGaa                                                  >  4:259175/1‑149 (MQ=255)
                                                                                                        accacAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTtctc                                            >  4:160517/1‑149 (MQ=255)
                                                                                                        accacAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTtct                                             <  3:76723/148‑1 (MQ=255)
                                                                                                          cacaATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCGGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTa                                          <  5:248026/149‑1 (MQ=255)
                                                                                                              aTGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAg                                       >  3:115601/1‑148 (MQ=255)
                                                                                                                gACAAGAGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGATGGCGTCTTCAATGCCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCAt                                    >  4:144897/1‑149 (MQ=255)
                                                                                                                  cAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCg                                  <  4:172192/149‑1 (MQ=255)
                                                                                                                  cAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCCGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCg                                  <  8:220374/149‑1 (MQ=255)
                                                                                                                   aaGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCgg                                 <  4:115601/149‑1 (MQ=255)
                                                                                                                         gcgcCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGAttt                           >  8:299367/1‑149 (MQ=255)
                                                                                                                         gcgcCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGAttt                           >  3:22010/1‑149 (MQ=255)
                                                                                                                          cgcCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGAtttt                          <  2:240470/149‑1 (MQ=255)
                                                                                                                           gcCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTg                         >  2:227548/1‑149 (MQ=255)
                                                                                                                             ccACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACg                                                       <  5:283145/117‑1 (MQ=255)
                                                                                                                             ccACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACg                                                       >  6:283145/1‑117 (MQ=255)
                                                                                                                             ccACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGcc                       <  2:21653/149‑1 (MQ=255)
                                                                                                                                 tATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGc                                                                                      <  3:150006/82‑1 (MQ=255)
                                                                                                                                 tATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGc                                                                                      >  4:150006/1‑82 (MQ=255)
                                                                                                                                   ttGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGtt                  <  8:305701/148‑1 (MQ=255)
                                                                                                                                   ttGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGt                   <  7:123477/147‑1 (MQ=255)
                                                                                                                                      agaATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGa                                                            <  7:204859/103‑1 (MQ=255)
                                                                                                                                      agaATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGa                                                            >  8:204859/1‑103 (MQ=255)
                                                                                                                                      agaATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTtacg              >  8:148232/1‑149 (MQ=255)
                                                                                                                                         aTAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGACCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTtacgtac           <  3:144897/149‑1 (MQ=255)
                                                                                                                                                 cagccgCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCgg   <  3:55711/149‑1 (MQ=255)
                                                                                                                                                 cagcagCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCgg   >  6:75622/1‑149 (MQ=255)
                                                                                                                                                  agcagcGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGgt  >  2:51691/1‑149 (MQ=255)
                                                                                                                                                  agcagcGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGgt  <  6:165217/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCCTGATCGGAAATACCTTCGCGCGCATCAATCACTAACATCACCACGTTGGCGTCTTCAATGGCCTGCAACGTTTTGATTACGGAGAATTTCTCTACAGCATCGGTGATTTTGCCGCGTTTACGTACGCCAGCGGT  >  NC_000913/2636294‑2636588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: