Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,971	A→G	Q201R (CAG→CGG)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,971	0	A	G	86.2%	249.1 / 24.7	94	Q201R (CAG→CGG)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base A (7/6);  new base G (42/39);  total (49/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCCGCTGCGCAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCA  >  NC_000913/1806827‑1807115                                                                                                                                                 |                                                                                                                                                 tttCCGCTGCGCAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTCAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCg                                                                                                                                              <  7:322405/149‑1 (MQ=255)      gCTGCGCAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGa                                                                                                                                         >  2:456001/1‑149 (MQ=255)          cgcAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGt                                                                                                                                      <  2:409522/148‑1 (MQ=255)             aaaaaCAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACAGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGAGAACTCACCCAGCCGGACGATGTCCg                                                                                                                                   >  3:1149/1‑148 (MQ=255)                  cAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGc                                                                                                                             >  3:79835/1‑149 (MQ=255)                      ggATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCcgcg                                                                                                                         <  1:233399/149‑1 (MQ=255)                       gATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCcgcg                                                                                                                         >  8:280370/1‑148 (MQ=255)                          ccGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAg                                                                                                                      >  5:403930/1‑148 (MQ=255)                                cATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGAt                                                                                                                                        <  2:214743/124‑1 (MQ=255)                                cATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATc                                                                                                                <  6:403930/148‑1 (MQ=255)                                cATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCg                                                                                                               >  7:290968/1‑149 (MQ=255)                                 aTCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCg                                                                                                               >  7:198564/1‑148 (MQ=255)                                 aTCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCg                                                                                                               <  8:198564/148‑1 (MQ=255)                                  tcgtcgACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGtt                                                                                                             <  2:185318/149‑1 (MQ=255)                                  tcgtcgACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGtt                                                                                                             <  6:311501/149‑1 (MQ=255)                                    gtcgACAGTTCTGGCGAAGCGTTAAGTGCTGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTaa                                                                                                           <  1:146031/149‑1 (MQ=255)                                    gtcgACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTa                                                                                                            <  6:135153/148‑1 (MQ=255)                                       gACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAg                                                                                                        >  1:289245/1‑149 (MQ=255)                                        aCAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGc                                                                                                       >  8:394702/1‑149 (MQ=255)                                         cAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGaaa                                                                                                                  >  4:299672/1‑137 (MQ=255)                                         cAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGaaa                                                                                                                  <  3:299672/137‑1 (MQ=255)                                         cAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCg                                                                                                      >  8:31051/1‑149 (MQ=255)                                           gTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGc                                                                                                    <  2:62554/149‑1 (MQ=255)                                            ttCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCaaa                                                                                                                               >  7:41173/1‑121 (MQ=255)                                            ttCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCaaa                                                                                                                               <  8:41173/121‑1 (MQ=255)                                               tGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGtt                                                                                                             <  5:91474/136‑1 (MQ=255)                                               tGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGtt                                                                                                             >  6:91474/1‑136 (MQ=255)                                                ggCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCg                                                                                                      <  6:338163/142‑1 (MQ=255)                                                ggCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCg                                                                                                      >  5:338163/1‑142 (MQ=255)                                                   gAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAg                                                                                                        <  8:122246/137‑1 (MQ=255)                                                   gAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAg                                                                                                        >  7:122246/1‑137 (MQ=255)                                                         ttAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGt                                                                                      >  1:45179/1‑149 (MQ=255)                                                            aGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGt                                                                                   <  4:79835/149‑1 (MQ=255)                                                                 agcaCTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGttt                                                                              >  6:185201/1‑149 (MQ=255)                                                                 agcaCTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGttt                                                                              <  5:185201/149‑1 (MQ=255)                                                                        gCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGAGGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTgg                                                                        <  5:115799/148‑1 (MQ=255)                                                                            ttGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGtt                                                                                                             <  7:447503/107‑1 (MQ=255)                                                                            ttGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGtt                                                                                                             >  8:447503/1‑107 (MQ=255)                                                                              ggTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCg                                                                                                               <  3:172054/103‑1 (MQ=255)                                                                              ggTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCg                                                                                                               >  4:172054/1‑103 (MQ=255)                                                                              ggTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCcac                                                                  >  2:460584/1‑148 (MQ=255)                                                                              ggTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCcac                                                                  <  7:280370/148‑1 (MQ=255)                                                                              ggTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCcac                                                                  >  8:134204/1‑148 (MQ=255)                                                                               gTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCcac                                                                  >  6:297641/1‑147 (MQ=255)                                                                               gTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTGGTCGTTTCCCTGGGTCcac                                                                  >  8:128876/1‑147 (MQ=255)                                                                                tAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAg                                                               >  8:57711/1‑149 (MQ=255)                                                                                tAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAg                                                               >  4:176843/1‑149 (MQ=255)                                                                                  aCATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAg                                                               >  2:460569/1‑147 (MQ=255)                                                                                  aCATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGa                                                             <  2:289245/149‑1 (MQ=255)                                                                                    aTCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAgc                                                           >  5:287807/1‑149 (MQ=255)                                                                                        aGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTg                                                       >  2:34487/1‑149 (MQ=255)                                                                                         gTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTgg                                                      <  1:456001/149‑1 (MQ=255)                                                                                         gTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTgg                                                      <  6:287807/149‑1 (MQ=255)                                                                                                 aGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGa                                               <  8:290968/148‑1 (MQ=255)                                                                                                  gCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGa                                               >  8:330714/1‑147 (MQ=255)                                                                                                   ccTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAg                                            >  6:260203/1‑149 (MQ=255)                                                                                                    cTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGAGAACTCACCCGGCCCGACGATATCCGCAAAGCAGCGCAGGAAATCGTTAATAGCGGCAAGACCAAACGGGTAGACGTTTCCCAGCGTCCACAAGCAGCGCTGGGTGAAGATa                                             >  2:415544/1‑147 (MQ=255)                                                                                                     tAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGt                                           >  7:4527/1‑148 (MQ=255)                                                                                                      aaCCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGt                                                                                   >  1:272814/1‑107 (MQ=255)                                                                                                      aaCCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGt                                                                                   <  2:272814/107‑1 (MQ=255)                                                                                                        ccAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaa                                        >  5:57095/1‑148 (MQ=255)                                                                                                        ccAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaa                                        >  1:205758/1‑148 (MQ=255)                                                                                                         cAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACggg                                                                                       >  3:346579/1‑100 (MQ=255)                                                                                                         cAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACggg                                                                                       <  4:346579/100‑1 (MQ=255)                                                                                                         cAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGt                                           <  8:435588/144‑1 (MQ=255)                                                                                                         cAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGt                                           >  7:435588/1‑144 (MQ=255)                                                                                                          aaaacGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGa                                               <  5:80062/139‑1 (MQ=255)                                                                                                          aaaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGa                                               >  6:80062/1‑139 (MQ=255)                                                                                                          aaaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAAc                                     >  5:159621/1‑149 (MQ=255)                                                                                                           aaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACt                                    <  7:134204/149‑1 (MQ=255)                                                                                                            aaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTg                                   <  1:460584/149‑1 (MQ=255)                                                                                                              aGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTa                                 <  8:4527/149‑1 (MQ=255)                                                                                                              aGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTa                                 <  5:297641/149‑1 (MQ=255)                                                                                                               gAACTCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTAt                                <  7:31051/149‑1 (MQ=255)                                                                                                                   tCAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAg                            >  2:194986/1‑149 (MQ=255)                                                                                                                   acAGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACCATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGAAAGGCCAAACGGGTTGTCGATTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAg                            >  1:301547/2‑149 (MQ=255)                                                                                                                     aGTGCGCTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAggt                          >  3:38387/1‑149 (MQ=255)                                                                                                                      gTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAggtg                         <  7:330714/149‑1 (MQ=255)                                                                                                                         cgcTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTAtt                               >  7:441261/1‑140 (MQ=255)                                                                                                                         cgcTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTAtt                               <  8:441261/140‑1 (MQ=255)                                                                                                                         cgcTGGTGAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAggtggt                       <  1:460569/148‑1 (MQ=255)                                                                                                                           cTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGTTGTTGATAGTGAAAACTGTATTCAGGTGGTGc                     >  3:387116/1‑148 (MQ=255)                                                                                                                              gTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGccacc                 <  4:1149/149‑1 (MQ=255)                                                                                                                                gAATCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGATAATAGCGGCAAGGACAAACGGGTTGTCGTTTCACTGGGTACACAATGAGCGCTGGGTGTTGATAGTTAAAACTGTATTCAGGTGGTGCcaccac               >  6:151783/1‑149 (MQ=255)                                                                                                                                 aaTCGCGAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATACTGAAAACTGTATTCAGGTGGTGCCaccacc              >  1:129459/1‑149 (MQ=255)                                                                                                                                  aTCGCGAACTCACCCGGCCGGACGTTGTCCGCAACGCCGCGCAGGAAATCGTTTATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGGCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCg             <  6:437194/149‑1 (MQ=255)                                                                                                                                    cgcgAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCTCTGGGTGTTGATAGTGAAAACTCGATTCAGGTGGTGCCACCACCGGt           >  6:127956/1‑149 (MQ=255)                                                                                                                                     gcgAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGGCaccacctgaa          <  7:394702/149‑5 (MQ=255)                                                                                                                                     gcgAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTg          <  2:129459/149‑1 (MQ=255)                                                                                                                                      cgAACTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTg          >  6:115513/1‑148 (MQ=255)                                                                                                                                        aaCTCACCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGaaa       <  3:176843/149‑1 (MQ=255)                                                                                                                                             aCCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGc    >  7:397410/1‑147 (MQ=255)                                                                                                                                             aCCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCa  <  2:45179/149‑1 (MQ=255)                                                                                                                                             aCCCGGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCa  <  4:31858/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                 TTTCCGCTGCGCAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCA  >  NC_000913/1806827‑1807115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑