New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 421984728 (0.750)5 (0.150) 5/226 4.0 17.2% coding (1252/1737 nt) aceK isocitrate dehydrogenase kinase/phosphatase
?NC_000913 = 4219854 23 (0.680)coding (1259/1737 nt) aceK isocitrate dehydrogenase kinase/phosphatase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4219690‑4219847
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atattgagcggcACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTG  <  NC_000913/4219854‑4219712
                                                                                                                                                                                                                                                                                                             
CGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATA                                                                                                                                                          >  2:84324/1‑149
       CGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAAAGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCG                                                                                                                                                   >  1:206728/1‑149
         AATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGC                                                                                                                                                 <  1:84324/149‑1
                          AGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCGTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCAGCTTTATATTGAGCGGCACCATCCGCCGCTCAAT                                                                                                                                <  5:149438/149‑1
                                                    CGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTT                                                                                                      >  8:99444/1‑149
                                                          ATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCAC                                                                                                              <  7:21463/135‑1
                                                          ATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCAC                                                                                                              >  8:21463/1‑135
                                                               CCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCG                                                                                           <  7:99444/149‑1
                                                                                                                                                     TTGAGCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCTTTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTC     >  2:206728/1‑149
                                                                                                                                                        AGCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTG  <  5:156602/149‑1
                                                                                                                                                        AGCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTG  <  7:28977/149‑1
                                                                                                                                                         GCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGC                               >  3:117704/1‑119
                                                                                                                                                         GCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGC                               <  4:117704/119‑1
                                                                                                                                                                                                                                                                                                             
CGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4219690‑4219847
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atattgagcggcACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTG  <  NC_000913/4219854‑4219712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.