Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,906,168	IS5 (+) +4 bp	coding (397‑400/837 nt)	bglG ←	transcriptional antiterminator of the bgl operon

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	28 (0.760)	26/246	0.6	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	3906168 =	0 (0.000)					coding (400/837 nt)	bglG	transcriptional antiterminator of the bgl operon
*	?	NC_000913	= 3653230	NA (NA)	50 (1.340)	47/250	0.0	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 3906171	0 (0.000)					coding (397/837 nt)	bglG	transcriptional antiterminator of the bgl operon

GACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3653086‑3653230 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTTGCAGGTGTCACGCAGTTAA  <  NC_000913/3906171‑3906030                                                                                                                                                                                                                                                                                                 GACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTA                                                                                                                                             >  1:195585/1‑148  ACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGG                                                                                                                                           <  1:130157/149‑1    AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGG                                                                                                                                          >  5:125577/1‑148      GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAA                                                                                                                                       <  6:125577/149‑1      GAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAACTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGG                                                                                                                                         >  6:119287/1‑147                  TCTGGTCTGACGGCGCTTACTGCTGAACTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAAC                                                                                                                            <  5:119287/148‑1                          GACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGA                                                                                                                   >  8:150920/1‑149                           ACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGA                                                                                                                   >  8:153940/1‑148                                    ACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTG                                                                                                         >  4:106534/1‑149                                       GCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGC                                                                                                      >  4:71353/1‑149                                           AATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGC                                                                                                  <  3:71353/149‑1                                              TCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGAT                                                                                                                  <  4:71412/130‑1                                              TCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGAT                                                                                                                  >  3:71412/1‑130                                                           AGGTAATTTGATGACTCATGATTAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCATGTACTTGTTCGCACCTTCCCTAGGGGAAGAATCTTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACAGAAAGATG                                                                                   >  8:133899/1‑148                                                                  TTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGC                                                                           >  3:9606/1‑149                                                                      TGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTA                                                                       <  7:150920/149‑1                                                                        ACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATT                                                                     <  7:153940/149‑1                                                                            ATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCA                                                                 <  7:133899/149‑1                                                                                    CCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGC                                                                   >  6:125112/1‑139                                                                                    CCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGC                                                                   <  5:125112/139‑1                                                                                     CCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGG                                                        >  2:51008/1‑149                                                                                     CCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCA                                                                                                 >  3:96047/1‑108                                                                                     CCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCA                                                                                                 <  4:96047/108‑1                                                                                      CTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGT                                                       <  1:51008/149‑1                                                                                      CTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCAT                                                                <  1:79259/140‑1                                                                                      CTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCAT                                                                >  2:79259/1‑140                                                                                            TATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGC                                                 >  7:18048/1‑149                                                                                             ATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTG                                                         >  7:80170/1‑140                                                                                             ATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTG                                                         <  8:80170/140‑1                                                                                                 CTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAA                                            >  7:56202/1‑149                                                                                                      GATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATT                                                                     >  1:165611/1‑119                                                                                                      GATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATT                                                                     <  2:165611/119‑1                                                                                                          ACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAA                                   <  4:9606/149‑1                                                                                                            AAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCA                                                             >  4:47387/1‑121                                                                                                            AAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCA                                                             <  3:47387/121‑1                                                                                                              ACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATAT                               >  5:103669/1‑149                                                                                                               CATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATG                              <  8:18048/149‑1                                                                                                                    TCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAAT                                           >  4:15769/1‑131                                                                                                                    TCTCATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAAT                                           <  3:15769/131‑1                                                                                                                       CATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGT                                                       <  1:96905/116‑1                                                                                                                       CATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGT                                                       >  2:96905/1‑116                                                                                                                        ATATCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTT                     >  5:182037/1‑149                                                                                                                           TCAGGGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTTGCA                  <  3:106534/149‑1                                                                                                                               GGACTTGTTCGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTTGCAGGTG              >  5:174246/1‑149                                                                                                                                        CGCACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTTGCAGGTGTCACGCAG      <  6:182037/148‑1                                                                                                                                           ACCTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAACGAGCGGAAATATGGAGGATGTTGCAGGTGTCACGCAGTTAA  >  8:66142/1‑149                                                                                                                                             CTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTTGCAG                 >  2:21356/1‑132                                                                                                                                             CTTCCCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTTGCAG                 <  1:21356/132‑1                                                                                                                                                 CCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGC                                                              >  6:17020/1‑83                                                                                                                                                 CCTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGC                                                              <  5:17020/83‑1                                                                                                                                                                                                                                                                                                 GACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3653086‑3653230 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAGGGGAAGAAGCGTTAACCATTATTGATAAACGGTTGGGCGTGCAGTTACCGAAAGATGAAGTGGGCTTTATTGCCATGCATCTGGTCAGTGCCCAAATGAGCGGAAATATGGAGGATGTTGCAGGTGTCACGCAGTTAA  <  NC_000913/3906171‑3906030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.