New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 23256454 (1.450)8 (0.240) 8/226 2.9 13.8% intergenic (+15/+33) yafE/mltD putative S‑adenosyl‑L‑methionine‑dependent methyltransferase/putative membrane‑bound lytic murein transglycosylase D
?NC_000913 = 232571 51 (1.510)intergenic (+22/+26) yafE/mltD putative S‑adenosyl‑L‑methionine‑dependent methyltransferase/putative membrane‑bound lytic murein transglycosylase D
Rejected: Frequency below/above cutoff threshold.

TTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGT  >  NC_000913/232437‑232711
                                                                                                                                      |                                                                                                                                            
ttCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTtta                                                                                                                                 <  1:184169/148‑1 (MQ=255)
   gCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTtattat                                                                                                                             >  5:76500/1‑149 (MQ=255)
   gCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTtattat                                                                                                                             >  4:133670/1‑149 (MQ=255)
          ccAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGttt                                                                                                                      <  6:76500/149‑1 (MQ=255)
              cAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCa                                                                                                                  <  3:133670/149‑1 (MQ=255)
                                  gagaACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTg                                                                                                          >  2:5723/1‑137 (MQ=255)
                                  gagaACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTg                                                                                                          <  1:5723/137‑1 (MQ=255)
                                                  ttGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGc                                                                                                        <  4:68602/123‑1 (MQ=255)
                                                  ttGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGc                                                                                                        >  3:68602/1‑123 (MQ=255)
                                                  ttGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCAACGGGGTAATAGGTGCATTTTTATTATCTGGTTTTTCAGGAATATGGCATGTTGTTGTTTTTCAAAAACAACg                                                                               >  8:49983/1‑148 (MQ=255)
                                                    gCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTg                                                                                                          <  1:72436/119‑1 (MQ=255)
                                                    gCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTg                                                                                                          >  2:72436/1‑119 (MQ=255)
                                                      aGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGc                                                                          <  1:162261/149‑1 (MQ=255)
                                                       gAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAga                                                                          >  3:163636/1‑147 (MQ=255)
                                                         atgatgGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTa                                                                       >  2:48355/1‑149 (MQ=255)
                                                               gCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGcc                                                                  <  1:48355/148‑1 (MQ=255)
                                                                cTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgttgtt                                                                                                >  7:50743/1‑117 (MQ=255)
                                                                cTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgttgtt                                                                                                <  8:50743/117‑1 (MQ=255)
                                                                cTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTg                                                                >  1:147840/1‑149 (MQ=255)
                                                                       aCCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGTCACCGGGGGACTCGGTGCCTTTTTATTATCTGGTTTGTCAGTAATCTGGCATGTGGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCa                                                          <  7:49983/148‑1 (MQ=255)
                                                                          aGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGAtt                                                      >  6:73216/1‑149 (MQ=255)
                                                                            tGATATCATCATGGTAGATGCTCATAAATAGGGATAAATAAATAAGGCACCGGGGGAATCGGTTCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTg                                                                <  2:100357/137‑1 (MQ=255)
                                                                            tGATATCATCATGGTAGATGCACATAAAGAGGCATAAATAAAAAAGGCACAGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCCGCTTATCGCCTg                                                                >  1:100357/1‑137 (MQ=255)
                                                                              atatCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgttg                                                                                                  <  5:14058/101‑1 (MQ=255)
                                                                              atatCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgttg                                                                                                  >  6:14058/1‑101 (MQ=255)
                                                                              atatCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCg                                                    >  7:32766/1‑147 (MQ=255)
                                                                                atcatcATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAgt                                                >  8:78331/1‑149 (MQ=255)
                                                                                  catcatGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgt                                                                                                    <  3:45948/95‑1 (MQ=255)
                                                                                  catcatGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgt                                                                                                    >  4:45948/1‑95 (MQ=255)
                                                                                   atcatGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAAt                                                                                                                                              >  3:8844/1‑52 (MQ=255)
                                                                                   atcatGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAAt                                                                                                                                              <  4:8844/52‑1 (MQ=255)
                                                                                       tGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATcggtgccttttttatttatgccgctttatgtgcatctaccatgatgatatcactggtgaaaaagccatcattctgcaaggcaaaatacgttctcacctctg                                         <  2:165180‑M2/149‑102 (MQ=255)
                                                                                          tAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCacaaaca                                                                                  <  4:41479/105‑1 (MQ=255)
                                                                                          tAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCacaaaca                                                                                  >  3:41479/1‑105 (MQ=255)
                                                                                            gATGCACATAAAGCGGCATAAATAAAAAATGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCa                                    <  4:163636/149‑1 (MQ=255)
                                                                                              tGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAgc                                  <  8:32766/149‑1 (MQ=255)
                                                                                                  caTAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATcggtgccttttttatttatgccgctttatgtgcatctaccatgatgatatcactggtgaaaaagccatcattctgcaaggcaaaatacgttctcacctctgtcgatgcgctc                              >  1:165180‑M2/1‑37 (MQ=255)
                                                                                                   aTAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCAt                              >  1:13975/1‑148 (MQ=255)
                                                                                                     aaaGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCa                                                  <  8:98120/126‑1 (MQ=255)
                                                                                                     aaaGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCa                                                  >  7:98120/1‑126 (MQ=255)
                                                                                                     aaaGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAgt                                                <  1:117682/128‑1 (MQ=255)
                                                                                                     aaaGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAgt                                                >  2:117682/1‑128 (MQ=255)
                                                                                                             ataaataaAAAAGGCACCGGGGGAATcggtgccttttttatttatgccgctttatgtgcatctaccatgatgatatcactggtgaaaaagccatcattctgc                                                                  <  5:99998‑M2/102‑77 (MQ=255)
                                                                                                             ataaataaAAAAGGCACCGGGGGAATcggtgccttttttatttatgccgctttatgtgcatctaccatgatgatatcactggtgaaaaagccatcattctgc                                                                  >  6:99998‑M2/1‑26 (MQ=255)
                                                                                                               aaataaaAAAGGCACCGGGGGAATcggtgccttttttatttatgccgctttatgtgcatctaccatgatgatatcactggtgaaaaagccatc                                                                         <  4:161006‑M2/93‑70 (MQ=255)
                                                                                                               aaataaaAAAGGCACCGGGGGAATcggtgccttttttatttatgccgctttatgtgcatctaccatgatgatatcactggtgaaaaagccatc                                                                         >  3:161006‑M2/1‑24 (MQ=255)
                                                                                                                  taaaAAAGGCACCGGGGGAATcggtgccttttttatttatgccgctttatgtgcatctaccatgatgatatcactggtgaaaaagccatcattctgcaa                                                                <  4:12568‑M2/99‑79 (MQ=255)
                                                                                                                  taaaAAAGGCACCGGGGGAATcggtgccttttttatttatgccgctttatgtgcatctaccatgatgatatcactggtgaaaaagccatcattctgcaa                                                                >  3:12568‑M2/1‑21 (MQ=255)
                                                                                                                     aaaaGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTc                                      <  6:100808/122‑1 (MQ=255)
                                                                                                                     aaaaGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTc                                      >  5:100808/1‑122 (MQ=255)
                                                                                                                       aaGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGcc         >  3:121843/1‑149 (MQ=255)
                                                                                                                         ggCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGt       <  5:73216/149‑1 (MQ=255)
                                                                                                                            aCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATc                             >  1:117006/1‑124 (MQ=255)
                                                                                                                            aCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATc                             <  2:117006/124‑1 (MQ=255)
                                                                                                                            aCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCtt                      <  7:33901/131‑1 (MQ=255)
                                                                                                                            aCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCtt                      >  8:33901/1‑131 (MQ=255)
                                                                                                                            aCCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGc    <  2:147840/149‑1 (MQ=255)
                                                                                                                             ccGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCg   >  2:137328/1‑149 (MQ=255)
                                                                                                                              cGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTGGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGt  <  7:78331/149‑1 (MQ=255)
                                                                                                                                ggggAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCAc            >  6:95435/1‑137 (MQ=255)
                                                                                                                                ggggAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCAc            <  5:95435/137‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                            
TTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGT  >  NC_000913/232437‑232711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.