Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 666,687 Δ1 bp 5.4% coding (1531/1902 nt) mrdA ← penicillin‑binding protein 2, transpeptidase involved in peptidoglycan synthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913666,6870A. 5.4% 248.0 / 6.6 74coding (1531/1902 nt)mrdApenicillin‑binding protein 2, transpeptidase involved in peptidoglycan synthesis
Reads supporting (aligned to +/- strand):  ref base A (38/32);  new base . (2/2);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

ATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGA  >  NC_000913/666541‑666830
                                                                                                                                                  |                                                                                                                                               
ataGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAAc                                                                                                                                               >  3:130494/1‑149 (MQ=255)
 taGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAAcc                                                                                                                                              >  7:17732/1‑149 (MQ=255)
    gTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTGATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTACCGgaa                                                                                                                                          >  5:249067/1‑149 (MQ=255)
        cgtgcgCTTGCAGACCGAAGACCTGAGCGGTACCTGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTACCGgaatga                                                                                                                                       <  6:249067/148‑1 (MQ=255)
               tttCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGc                                                                                                                                >  7:359480/1‑149 (MQ=255)
                   cgACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGGGCGCTAGCAAAGTATTTATGCGCCGTACCGCTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGa                                                                                                                             <  4:264521/147‑1 (MQ=255)
                    gACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACg                                                                                                                           >  1:392239/1‑149 (MQ=255)
                     aCCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGACGACgg                                                                                                                          >  7:159646/1‑149 (MQ=255)
                       cGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGc                                                                                                                        >  1:236856/1‑149 (MQ=255)
                         aaGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCgg                                                                                                                      >  8:115895/1‑149 (MQ=255)
                          ataCCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGAGCTATCAAAGGATTTATGCGCCGTACCGTTAGGGCGGTTATAAACACAGTACATACCGACTTACGACAGCTCCAAGAAACAGGAATGAATAAAGCCGACGGGCGGt                                                                                                                     >  2:334738/3‑149 (MQ=255)
                          aGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATCCCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGt                                                                                                                     >  2:296015/1‑149 (MQ=255)
                              cTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACAGCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCa                                                                                                                  <  8:295721/148‑1 (MQ=255)
                              cTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCAt                                                                                                                 >  4:243073/1‑149 (MQ=255)
                               tGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATg                                                                                                                >  4:239525/1‑149 (MQ=255)
                                 aGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCg                                                                                                              <  7:198579/149‑1 (MQ=255)
                                  gCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCgg                                                                                                             <  2:359142/149‑1 (MQ=255)
                                    ggTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCt                                                                                                           <  8:359480/149‑1 (MQ=255)
                                       aCCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTa                                                                                                        >  7:59067/1‑149 (MQ=255)
                                        ccGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTAc                                                                                                       <  6:362765/149‑1 (MQ=255)
                                               tCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATgg                                                                                                 <  7:272637/148‑1 (MQ=255)
                                                cgccgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCa                                                                                               >  3:331427/1‑149 (MQ=255)
                                                cgccgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCa                                                                                               >  3:336833/1‑149 (MQ=255)
                                                  ccgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACGGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCAcc                                                                                             >  3:82371/1‑149 (MQ=255)
                                                   cgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTAc                                                                                                       <  6:20737/138‑1 (MQ=255)
                                                   cgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTAc                                                                                                       >  5:20737/1‑138 (MQ=255)
                                                   cgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCt                                                                                            >  4:372798/1‑149 (MQ=255)
                                                   cgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCt                                                                                            >  4:246531/1‑149 (MQ=255)
                                                   cgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCt                                                                                            >  3:33062/1‑149 (MQ=255)
                                                      aaTTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATAACGTCTTTCGGCAGCTCCCAGTAACCGGAATGAATATCGCCGTCGGGCTGTTCATGCGGCGGTACCCATGGCACCTGtt                                                                                         <  1:334738/149‑1 (MQ=255)
                                                         tttGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGc                                                                                      >  1:158229/1‑149 (MQ=255)
                                                         tttGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGcc                                                                                     >  8:240879/1‑149 (MQ=255)
                                                            gTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCg                                                                                    <  4:331427/148‑1 (MQ=255)
                                                            gTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCg                                                                                    <  4:336833/148‑1 (MQ=255)
                                                               cGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCtt                                                                                <  6:100179/149‑1 (MQ=255)
                                                                    cgcTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCGGTACCCATGGCACCTGTTTGCCGTCTTcggcg                                                                           <  3:243073/149‑1 (MQ=255)
                                                                      cTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGt                                                                         <  2:18548/149‑1 (MQ=255)
                                                                       tAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTg                                                                        >  4:9430/1‑149 (MQ=255)
                                                                         gCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCCCCTGTTTGCCGTCtt                                                                                >  5:70731/1‑139 (MQ=255)
                                                                         gCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCtt                                                                                <  6:70731/139‑1 (MQ=255)
                                                                          cAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCt                                                                      >  3:208091/1‑148 (MQ=255)
                                                                          cAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCt                                                                      >  7:41715/1‑148 (MQ=255)
                                                                           aaaGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtca                                                                    >  7:319835/1‑149 (MQ=255)
                                                                           aaaGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtca                                                                    >  2:22380/1‑149 (MQ=255)
                                                                            aaGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtcat                                                                   >  1:245346/1‑149 (MQ=255)
                                                                            aaGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtcat                                                                   >  4:13162/1‑149 (MQ=255)
                                                                             aGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtcatc                                                                  <  3:246531/149‑1 (MQ=255)
                                                                             aGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtcatc                                                                  <  3:9430/149‑1 (MQ=255)
                                                                             aGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTACCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtcat                                                                   >  5:26000/1‑148 (MQ=255)
                                                                              gTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtcatca                                                                 <  5:316849/149‑1 (MQ=255)
                                                                              gTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtcatca                                                                 <  3:239525/149‑1 (MQ=255)
                                                                                  ttATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCa                                                              >  8:358000/1‑148 (MQ=255)
                                                                                     tGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAAt                                                           <  7:115895/148‑1 (MQ=255)
                                                                                       cgcCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAg                                                        <  1:296015/149‑1 (MQ=255)
                                                                                        gcCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAg                                                        >  5:366544/1‑148 (MQ=255)
                                                                                          cGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGaa                                                     <  3:13162/149‑1 (MQ=255)
                                                                                             aCCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAAcc                                                   >  2:43114/1‑148 (MQ=255)
                                                                                              cccatAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGTCCGGTTCATGCGGCTGTACCCATCGAACCTGTTTGCCGTCTTCGGCGATGCTCATCAGCAAATGAGGAACCt                                                  >  6:117047/5‑148 (MQ=255)
                                                                                                gTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCAc                                              <  7:240879/149‑1 (MQ=255)
                                                                                                              cAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCAtt                                  >  2:327583/1‑148 (MQ=255)
                                                                                                               aaCACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTaa                                >  1:18041/1‑149 (MQ=255)
                                                                                                                  acCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCa                             <  2:158229/149‑1 (MQ=255)
                                                                                                                   ccGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAg                            <  8:59067/149‑1 (MQ=255)
                                                                                                                           aCCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGatcat                      >  1:328456/1‑147 (MQ=255)
                                                                                                                            ccGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGAtcatca                    <  2:245346/148‑1 (MQ=255)
                                                                                                                             cGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGt                  <  2:392239/149‑1 (MQ=255)
                                                                                                                               tcattCGCCAGCTCCCAGTAACCGGAATGAATAGCTCCGACCGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCATCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGc                <  6:300109/146‑1 (MQ=255)
                                                                                                                               tCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGc                <  4:130494/149‑1 (MQ=255)
                                                                                                                                    cGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTa            <  2:236856/148‑1 (MQ=255)
                                                                                                                                          cTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCt     <  8:17732/149‑1 (MQ=255)
                                                                                                                                           tCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTg    >  6:142175/1‑149 (MQ=255)
                                                                                                                                             ccAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGa  <  1:43114/149‑1 (MQ=255)
                                                                                                                                               aGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATc      >  6:208723/1‑143 (MQ=255)
                                                                                                                                               aGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATc      <  5:208723/143‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAATCAGGATCATCAGTGCCTTACTCATCTGGA  >  NC_000913/666541‑666830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: