Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,408,537 A→T 5.9% E163V (GAG→GTG)  ydaN → putative Zn(II) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,408,5370AT 5.9% 250.1 / 4.3 84E163V (GAG→GTG) ydaNputative Zn(II) transporter
Reads supporting (aligned to +/- strand):  ref base A (45/33);  new base T (3/2);  total (49/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

GGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTT  >  NC_000913/1408390‑1408682
                                                                                                                                                   |                                                                                                                                                 
tgTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTTAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGag                                                                                                                                                  <  8:18647/148‑1 (MQ=255)
  ttAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGagca                                                                                                                                                <  5:179282/149‑1 (MQ=255)
      ttGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTg                                                                                                                                            >  3:333384/1‑149 (MQ=255)
      ttGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTg                                                                                                                                            >  3:258109/1‑149 (MQ=255)
          ttCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGCCCGATCATTCCAGTGAATTTATCGAGCAGCTGCAc                                                                                                                                         >  2:32354/1‑148 (MQ=255)
          ttCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGAGGCTGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCTCGCATCTGCAc                                                                                                                                         >  4:106870/1‑148 (MQ=255)
              aCCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATa                                                                                                                                     >  1:183282/1‑148 (MQ=255)
               cccGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGCCCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaa                                                                                                                                    >  2:307184/1‑148 (MQ=255)
                ccGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaaaa                                                                                                                                  <  1:394227/149‑1 (MQ=255)
                ccGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaaaa                                                                                                                                  >  8:148802/1‑149 (MQ=255)
                 cGACAACGCAAAGTGCTGGCGCTGGACGAAGTGGTGAGCGATCTGGAAGAGCGCACGGGTCCGAGCGATTGCGGCGGATGGCTCGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGAGaaaa                                                                                                                                  >  2:379074/1‑148 (MQ=255)
                    cAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGTGCAGCTGCTCGATaaattcac                                                                                                                              <  7:262684/149‑6 (MQ=255)
                    cAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTAt                                                                                                                              <  4:141384/149‑1 (MQ=255)
                          aaaGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCt                                                                                                                        <  6:259575/149‑1 (MQ=255)
                           aaGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCtt                                                                                                                       >  5:31229/1‑149 (MQ=255)
                           aaGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCtt                                                                                                                       >  5:373536/1‑149 (MQ=255)
                             gTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGa                                                                                                                     <  3:165981/149‑1 (MQ=255)
                             gTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGGGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGa                                                                                                                     <  1:307184/149‑1 (MQ=255)
                              tGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTg                                                                                                                      >  4:219999/1‑147 (MQ=255)
                                       tGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGTTTGCGGGGGATGGCTGGTGGATGTCTGCGAAGCGTTGACCGATCATTCCAGTGATTTTATCGAGCAGCGGCACGATAATATTATCGACCTTGAAGATAAtctc                                                                                                           <  8:105098/149‑1 (MQ=255)
                                        ggACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTcc                                                                                                          >  7:143140/1‑149 (MQ=255)
                                         gACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGGTGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAAtctc                                                                                                           <  8:176659/147‑1 (MQ=255)
                                         gACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTcc                                                                                                          <  8:118801/148‑1 (MQ=255)
                                          aCGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCtt                                                                                                        <  4:258109/149‑1 (MQ=255)
                                                    gAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATAGACCTTGAAGATAATCTCCTTGATCAGCaaa                                                                                              >  4:271140/1‑149 (MQ=255)
                                                     aGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAAt                                                                                             >  6:359018/1‑149 (MQ=255)
                                                        gATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTc                                                                                           >  5:101230/1‑148 (MQ=255)
                                                         aTCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGCCCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAATGTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTccc                                                                                         >  8:98959/1‑148 (MQ=255)
                                                         aTCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCa                                                                                         >  5:139491/1‑149 (MQ=255)
                                                          tCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGTGCAGCTGCTCGATaaattcac                                                                                                                              <  7:12617/111‑6 (MQ=255)
                                                          tCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGTGCAGCTGCTCGATaaattcac                                                                                                                              >  8:12617/1‑106 (MQ=255)
                                                          tCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCAc                                                                                        >  1:176970/1‑149 (MQ=255)
                                                          tCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCAc                                                                                        >  7:101546/1‑149 (MQ=255)
                                                              gAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCAc                                                                                        <  6:29069/145‑1 (MQ=255)
                                                              gAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACcgcg                                                                                    >  4:182098/1‑149 (MQ=255)
                                                              gAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACcgc                                                                                     <  7:68542/148‑1 (MQ=255)
                                                                agagGGCAAGGTTCCTTCCGTTTGCGTGTGATGGCTTGTGTATGTGTTCGATGCGTTGACCGATCATTAAAGTTAATTTATCGAGAAGCTGCACGATAAAATTTTCGACCTTGAAGATAATCTCCTTGATCTGCAAATTCCACAGCGTg                                                                                  <  8:330179/149‑1 (MQ=255)
                                                                    ggCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGAtt                                                                              >  3:315217/1‑149 (MQ=255)
                                                                      cACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGGTCAGCAAATTCCACCGCGTGGATTcc                                                                            >  8:209586/1‑149 (MQ=255)
                                                                      cACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGCTCAGCAAATTCCACCGCGTGGATTcc                                                                            <  7:209586/149‑1 (MQ=255)
                                                                       aCGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaaaa                                                                                                                                  <  5:138459/94‑1 (MQ=255)
                                                                       aCGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaaaa                                                                                                                                  >  6:138459/1‑94 (MQ=255)
                                                                       aCGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCt                                                                           >  8:369984/1‑149 (MQ=255)
                                                                          ggTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGc                                                                        <  8:101546/149‑1 (MQ=255)
                                                                          ggTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGc                                                                        <  3:312556/149‑1 (MQ=255)
                                                                            tCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGctc                                                                      <  3:282177/149‑1 (MQ=255)
                                                                              cgaccgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGctct                                                                     <  4:333384/148‑1 (MQ=255)
                                                                                accgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctgct                                                                  <  5:359018/149‑1 (MQ=255)
                                                                                  cgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCtgctgc                                                                <  1:360057/149‑1 (MQ=255)
                                                                                  cgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCtgctgc                                                                <  2:183282/149‑1 (MQ=255)
                                                                                         gggggATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAAc                                                          >  1:178383/1‑148 (MQ=255)
                                                                                            ggATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaatt                                                      <  4:315217/149‑1 (MQ=255)
                                                                                               tGGCTGGTGGATGTGTGCGATGCGTTGACCGCTCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaattaat                                                   <  4:81896/149‑1 (MQ=255)
                                                                                               tGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTGGATCAGCAAATTCCACCGCGTTGATTCCTGGCTCTGCTGCGCAAACaattaat                                                   <  6:373536/149‑1 (MQ=255)
                                                                                                 gCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaattaatt                                                  <  2:176970/148‑1 (MQ=255)
                                                                                                  cTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATtgt                                                <  1:271469/149‑1 (MQ=255)
                                                                                                   tggtggATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATtgt                                                >  5:384144/1‑148 (MQ=255)
                                                                                                           gtgtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATTCGTct                                       >  5:146372/1‑148 (MQ=255)
                                                                                                             gtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctg                                                                    <  2:1090/118‑1 (MQ=255)
                                                                                                             gtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctg                                                                    >  1:1090/1‑118 (MQ=255)
                                                                                                              tgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCta                                    >  2:333722/1‑149 (MQ=255)
                                                                                                              tgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTCGCTCTGCTGCGCAAACaattaatc                                                  >  4:196714/1‑134 (MQ=255)
                                                                                                                cGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCtata                                  <  6:31229/149‑1 (MQ=255)
                                                                                                                cGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCtata                                  <  7:148802/149‑1 (MQ=255)
                                                                                                                       ttGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCAcc                           >  5:42801/1‑149 (MQ=255)
                                                                                                                          aCCGATCATTCCAGTGAATTTATCGAGAAGCTGCACGATAAAATTATCGACCTTGAAGATAATATCCTAGATCAGAAAATTCCAACGCGTGGATTACTGGCTCTGCTGCGCAAACAATTAATTGTGATTAGTCGCTATATGGCAcctc                         >  8:123662/1‑146 (MQ=255)
                                                                                                                            cGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAAc                      >  8:332556/1‑149 (MQ=255)
                                                                                                                              aTCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGt                    >  3:90331/1‑149 (MQ=255)
                                                                                                                              aTCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGt                    >  6:348177/1‑149 (MQ=255)
                                                                                                                              aTCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTAGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGAAGCGTCGCTATATGGCACCGCacagt                    >  6:147242/1‑145 (MQ=255)
                                                                                                                               tCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGt                    >  1:358288/1‑148 (MQ=255)
                                                                                                                                 aTTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGa                  <  6:101230/148‑1 (MQ=255)
                                                                                                                                  ttCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATg                >  6:73608/1‑149 (MQ=255)
                                                                                                                                     cAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCGTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAAGTGTGATGCGTCGCTATATGGCACAGAAAGGTGATGtt              >  5:164785/1‑148 (MQ=255)
                                                                                                                                     cAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTGGATCAGCAAATTCCACCTCGGGGATTCCTGGCTCTGCTGCGCAAACAATGAATTGTGATGAGTCGCTATATGGACCCGCAACGTGATggt              >  4:10191/1‑146 (MQ=255)
                                                                                                                                      gataattttATCGTGCAGCTGCTCGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTa            >  3:155684/7‑149 (MQ=255)
                                                                                                                                       gTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTAt           >  1:63965/1‑149 (MQ=255)
                                                                                                                                       gTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTAt           >  1:10943/1‑149 (MQ=255)
                                                                                                                                       gTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTAt           >  6:97368/1‑149 (MQ=255)
                                                                                                                                       gTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTAt           <  6:384144/149‑1 (MQ=255)
                                                                                                                                        tGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATg          >  5:213462/1‑149 (MQ=255)
                                                                                                                                          attttATCGTGCAGCTGCTCGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCt        >  1:380838/3‑149 (MQ=255)
                                                                                                                                          aaTTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCt        >  6:271052/1‑149 (MQ=255)
                                                                                                                                           aTTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAATCAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTc       <  4:90331/149‑1 (MQ=255)
                                                                                                                                            tttATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGAGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGATATATGGCACCGCAACGTGATGTTTATGCTCg      >  4:57445/1‑149 (MQ=255)
                                                                                                                                                tCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGATATATGGCACCGCAACGTGATGTTTATGCTCGTCtt  <  7:273527/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTT  >  NC_000913/1408390‑1408682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑