Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,408,546 A→T 5.7% H166L (CAC→CTC)  ydaN → putative Zn(II) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,408,5460AT 5.7% 263.3 / 2.6 84H166L (CAC→CTC) ydaNputative Zn(II) transporter
Reads supporting (aligned to +/- strand):  ref base A (44/35);  new base T (3/2);  total (47/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

TTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCAGTGAACGT  >  NC_000913/1408400‑1408694
                                                                                                                                                  |                                                                                                                                                    
ttCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGCCCGATCATTCCAGTGAATTTATCGAGCAGCTGCAc                                                                                                                                                     >  2:32354/1‑148 (MQ=255)
ttCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGAGGCTGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCTCGCATCTGCAc                                                                                                                                                     >  4:106870/1‑148 (MQ=255)
    aCCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATa                                                                                                                                                 >  1:183282/1‑148 (MQ=255)
     cccGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGCCCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaa                                                                                                                                                >  2:307184/1‑148 (MQ=255)
      ccGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaaaa                                                                                                                                              >  8:148802/1‑149 (MQ=255)
      ccGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaaaa                                                                                                                                              <  1:394227/149‑1 (MQ=255)
       cGACAACGCAAAGTGCTGGCGCTGGACGAAGTGGTGAGCGATCTGGAAGAGCGCACGGGTCCGAGCGATTGCGGCGGATGGCTCGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGAGaaaa                                                                                                                                              >  2:379074/1‑148 (MQ=255)
          cAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGTGCAGCTGCTCGATaaattcac                                                                                                                                          <  7:262684/149‑6 (MQ=255)
          cAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTAt                                                                                                                                          <  4:141384/149‑1 (MQ=255)
                aaaGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCt                                                                                                                                    <  6:259575/149‑1 (MQ=255)
                 aaGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCtt                                                                                                                                   >  5:373536/1‑149 (MQ=255)
                 aaGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCtt                                                                                                                                   >  5:31229/1‑149 (MQ=255)
                   gTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGa                                                                                                                                 <  3:165981/149‑1 (MQ=255)
                   gTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGGGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGa                                                                                                                                 <  1:307184/149‑1 (MQ=255)
                    tGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTg                                                                                                                                  >  4:219999/1‑147 (MQ=255)
                             tGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGTTTGCGGGGGATGGCTGGTGGATGTCTGCGAAGCGTTGACCGATCATTCCAGTGATTTTATCGAGCAGCGGCACGATAATATTATCGACCTTGAAGATAAtctc                                                                                                                       <  8:105098/149‑1 (MQ=255)
                              ggACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTcc                                                                                                                      >  7:143140/1‑149 (MQ=255)
                               gACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGGTGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAAtctc                                                                                                                       <  8:176659/147‑1 (MQ=255)
                               gACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTcc                                                                                                                      <  8:118801/148‑1 (MQ=255)
                                aCGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCtt                                                                                                                    <  4:258109/149‑1 (MQ=255)
                                          gAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATAGACCTTGAAGATAATCTCCTTGATCAGCaaa                                                                                                          >  4:271140/1‑149 (MQ=255)
                                           aGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAAt                                                                                                         >  6:359018/1‑149 (MQ=255)
                                              gATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTc                                                                                                       >  5:101230/1‑148 (MQ=255)
                                               aTCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGCCCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAATGTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTccc                                                                                                     >  8:98959/1‑148 (MQ=255)
                                               aTCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCa                                                                                                     >  5:139491/1‑149 (MQ=255)
                                                tCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGTGCAGCTGCTCGATaaattcac                                                                                                                                          <  7:12617/111‑6 (MQ=255)
                                                tCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGTGCAGCTGCTCGATaaattcac                                                                                                                                          >  8:12617/1‑106 (MQ=255)
                                                tCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCAc                                                                                                    >  7:101546/1‑149 (MQ=255)
                                                tCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCAc                                                                                                    >  1:176970/1‑149 (MQ=255)
                                                    gAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCAc                                                                                                    <  6:29069/145‑1 (MQ=255)
                                                    gAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACcgcg                                                                                                >  4:182098/1‑149 (MQ=255)
                                                    gAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACcgc                                                                                                 <  7:68542/148‑1 (MQ=255)
                                                      agagGGCAAGGTTCCTTCCGTTTGCGTGTGATGGCTTGTGTATGTGTTCGATGCGTTGACCGATCATTAAAGTTAATTTATCGAGAAGCTGCACGATAAAATTTTCGACCTTGAAGATAATCTCCTTGATCTGCAAATTCCACAGCGTg                                                                                              <  8:330179/149‑1 (MQ=255)
                                                          ggCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGAtt                                                                                          >  3:315217/1‑149 (MQ=255)
                                                            cACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGGTCAGCAAATTCCACCGCGTGGATTcc                                                                                        >  8:209586/1‑149 (MQ=255)
                                                            cACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGCTCAGCAAATTCCACCGCGTGGATTcc                                                                                        <  7:209586/149‑1 (MQ=255)
                                                             aCGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaaaa                                                                                                                                              <  5:138459/94‑1 (MQ=255)
                                                             aCGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaaaa                                                                                                                                              >  6:138459/1‑94 (MQ=255)
                                                             aCGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCt                                                                                       >  8:369984/1‑149 (MQ=255)
                                                                ggTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGc                                                                                    <  3:312556/149‑1 (MQ=255)
                                                                ggTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGc                                                                                    <  8:101546/149‑1 (MQ=255)
                                                                  tCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGctc                                                                                  <  3:282177/149‑1 (MQ=255)
                                                                    cgaccgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGctct                                                                                 <  4:333384/148‑1 (MQ=255)
                                                                      accgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctgct                                                                              <  5:359018/149‑1 (MQ=255)
                                                                        cgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCtgctgc                                                                            <  2:183282/149‑1 (MQ=255)
                                                                        cgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCtgctgc                                                                            <  1:360057/149‑1 (MQ=255)
                                                                               gggggATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAAc                                                                      >  1:178383/1‑148 (MQ=255)
                                                                                  ggATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaatt                                                                  <  4:315217/149‑1 (MQ=255)
                                                                                     tGGCTGGTGGATGTGTGCGATGCGTTGACCGCTCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaattaat                                                               <  4:81896/149‑1 (MQ=255)
                                                                                     tGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTGGATCAGCAAATTCCACCGCGTTGATTCCTGGCTCTGCTGCGCAAACaattaat                                                               <  6:373536/149‑1 (MQ=255)
                                                                                       gCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaattaatt                                                              <  2:176970/148‑1 (MQ=255)
                                                                                        cTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATtgt                                                            <  1:271469/149‑1 (MQ=255)
                                                                                         tggtggATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATtgt                                                            >  5:384144/1‑148 (MQ=255)
                                                                                                 gtgtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATTCGTct                                                   >  5:146372/1‑148 (MQ=255)
                                                                                                   gtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctg                                                                                >  1:1090/1‑118 (MQ=255)
                                                                                                   gtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctg                                                                                <  2:1090/118‑1 (MQ=255)
                                                                                                    tgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCta                                                >  2:333722/1‑149 (MQ=255)
                                                                                                    tgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTCGCTCTGCTGCGCAAACaattaatc                                                              >  4:196714/1‑134 (MQ=255)
                                                                                                      cGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCtata                                              <  7:148802/149‑1 (MQ=255)
                                                                                                      cGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCtata                                              <  6:31229/149‑1 (MQ=255)
                                                                                                             ttGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCAcc                                       >  5:42801/1‑149 (MQ=255)
                                                                                                                aCCGATCATTCCAGTGAATTTATCGAGAAGCTGCACGATAAAATTATCGACCTTGAAGATAATATCCTAGATCAGAAAATTCCAACGCGTGGATTACTGGCTCTGCTGCGCAAACAATTAATTGTGATTAGTCGCTATATGGCAcctc                                     >  8:123662/1‑146 (MQ=255)
                                                                                                                  cGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAAc                                  >  8:332556/1‑149 (MQ=255)
                                                                                                                    aTCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGt                                >  3:90331/1‑149 (MQ=255)
                                                                                                                    aTCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGt                                >  6:348177/1‑149 (MQ=255)
                                                                                                                    aTCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTAGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGAAGCGTCGCTATATGGCACCGCacagt                                >  6:147242/1‑145 (MQ=255)
                                                                                                                     tCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGt                                >  1:358288/1‑148 (MQ=255)
                                                                                                                       aTTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGa                              <  6:101230/148‑1 (MQ=255)
                                                                                                                        ttCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATg                            >  6:73608/1‑149 (MQ=255)
                                                                                                                           cAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCGTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAAGTGTGATGCGTCGCTATATGGCACAGAAAGGTGATGtt                          >  5:164785/1‑148 (MQ=255)
                                                                                                                           cAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTGGATCAGCAAATTCCACCTCGGGGATTCCTGGCTCTGCTGCGCAAACAATGAATTGTGATGAGTCGCTATATGGACCCGCAACGTGATggt                          >  4:10191/1‑146 (MQ=255)
                                                                                                                            gataattttATCGTGCAGCTGCTCGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTa                        >  3:155684/7‑149 (MQ=255)
                                                                                                                             gTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTAt                       >  1:10943/1‑149 (MQ=255)
                                                                                                                             gTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTAt                       >  6:97368/1‑149 (MQ=255)
                                                                                                                             gTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTAt                       <  6:384144/149‑1 (MQ=255)
                                                                                                                             gTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTAt                       >  1:63965/1‑149 (MQ=255)
                                                                                                                              tGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATg                      >  5:213462/1‑149 (MQ=255)
                                                                                                                                attttATCGTGCAGCTGCTCGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCt                    >  1:380838/3‑149 (MQ=255)
                                                                                                                                aaTTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCt                    >  6:271052/1‑149 (MQ=255)
                                                                                                                                 aTTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAATCAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTc                   <  4:90331/149‑1 (MQ=255)
                                                                                                                                  tttATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGAGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGATATATGGCACCGCAACGTGATGTTTATGCTCg                  >  4:57445/1‑149 (MQ=255)
                                                                                                                                      tCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGATATATGGCACCGCAACGTGATGTTTATGCTCGTCtt              <  7:273527/149‑1 (MQ=255)
                                                                                                                                          gcagcTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCa          <  1:333722/149‑1 (MQ=255)
                                                                                                                                            agcTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCAGt        <  2:178383/149‑1 (MQ=255)
                                                                                                                                                  aCGATAAAATTTTCGACCTTGAAGATACTCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCAGTGAACGt  >  2:235665/1‑149 (MQ=255)
                                                                                                                                                  aCGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCAGTGAACGt  >  7:30741/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCAGTGAACGT  >  NC_000913/1408400‑1408694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: