Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,806,319 C→T 100% intergenic (‑236/‑51) ydiY ← / → pfkB acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,806,3190CT100.0% 254.2 / NA 76intergenic (‑236/‑51)ydiY/pfkBacid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/37);  total (39/37)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGC  >  NC_000913/1806174‑1806456
                                                                                                                                                 |                                                                                                                                         
aacgccttaaatggcgaagaaacggtctaaataggctgattcaatgcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTAc                                                                                                                                         <  3:210348‑M2/13‑1 (MQ=255)
aacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACt                                                                                                                                        >  5:38476‑M2/136‑149 (MQ=255)
     cttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCg                                                                                                                                   >  7:151913‑M2/131‑149 (MQ=255)
      ttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaataaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttcgccgcttattcgcaccttccTTAATTTCTTTACTTTc                                                                                                                                     <  5:114910‑M2/17‑1 (MQ=255)
      ttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaaaatgaagaaatgaaatgactgagtcagccgagaataatttcccagcttattcgcaccttccTTAATTTCATTACTTTc                                                                                                                                     >  6:114910‑M2/130‑146 (MQ=255)
       taaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCt                                                                                                                                 >  4:208959‑M2/129‑149 (MQ=255)
        aaatggcgaagaaacggtctaaataggctgattcaaggcatttacggtagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGc                                                                                                                                  <  6:350568‑M2/20‑1 (MQ=255)
         aatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGa                                                                                                                               <  3:82151‑M2/23‑1 (MQ=255)
         aatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGa                                                                                                                               <  8:151913‑M2/23‑1 (MQ=255)
              cgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCgg                                                                                                                          <  5:83275‑M2/28‑1 (MQ=255)
               gaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcagattccTTAATTTCTTTACTTTCCGCTCATTCGGt                                                                                                                         >  3:360125‑M2/121‑149 (MQ=255)
               gaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGc                                                                                                                                  <  8:349727‑M2/20‑1 (MQ=255)
               gaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGc                                                                                                                                  >  7:349727‑M2/121‑140 (MQ=255)
                   aaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGc                                                                                                                       >  5:258155‑M2/117‑147 (MQ=255)
                       ggtctaaataggctgattcaaggcatttacgggagaaaaactcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACt                                                                                                                 <  4:2267‑M2/37‑1 (MQ=255)
                         tctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTg                                                                                                                >  6:257298‑M2/111‑148 (MQ=255)
                             aataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAAt                                                                                                            >  8:319298‑M2/107‑148 (MQ=255)
                             aataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAAt                                                                                                            >  1:206258‑M2/107‑148 (MQ=255)
                                 ggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGcc                                                                                                       <  2:206258‑M2/47‑1 (MQ=255)
                                 ggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                                        <  3:109870‑M2/46‑1 (MQ=255)
                                 ggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                                        <  6:38476‑M2/46‑1 (MQ=255)
                                          aaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATaggag                                                                                               >  2:58104‑M2/94‑148 (MQ=255)
                                           aggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCtat                                                                                                    <  8:31050‑M2/50‑1 (MQ=255)
                                           aggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCtat                                                                                                    >  7:31050‑M2/93‑142 (MQ=255)
                                             gcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGaaa                                                                                           <  6:258155‑M2/59‑1 (MQ=255)
                                               atttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgcgtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAat                                                                                          >  5:64572‑M2/89‑148 (MQ=255)
                                               atttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAat                                                                                          >  7:300364‑M2/89‑148 (MQ=255)
                                                tttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                                        >  8:290253‑M2/88‑133 (MQ=255)
                                                tttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                                        <  7:290253‑M2/46‑1 (MQ=255)
                                                tttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatga                                                                                        <  6:12332‑M2/62‑1 (MQ=255)
                                                  tacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgat                                                                                       <  5:257298‑M2/63‑1 (MQ=255)
                                                    cgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATgg                                                                                     <  7:319298‑M2/65‑1 (MQ=255)
                                                       gagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGAGTCGGTGCCAGACTGAAATAAGCCTATAGGAGGAAATGATGGTTCg                                                                                 >  5:201378‑M2/81‑149 (MQ=255)
                                                        agaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACtt                                                                                                                                       >  2:268124‑M2/80‑94 (MQ=255)
                                                        agaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACtt                                                                                                                                       <  1:268124‑M2/15‑1 (MQ=255)
                                                          aaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTAt                                                                              >  7:55609‑M2/78‑149 (MQ=255)
                                                              aatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCtat                                                                          <  8:84220‑M2/76‑1 (MQ=255)
                                                                   gctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTg                                                                                                                        <  4:62586‑M2/30‑1 (MQ=255)
                                                                   gctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTg                                                                                                                        >  3:62586‑M2/69‑98 (MQ=255)
                                                                   gctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                     <  3:235796‑M2/81‑1 (MQ=255)
                                                                   gctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                     >  1:292607‑M2/69‑149 (MQ=255)
                                                                      caaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                                   >  5:150582‑M2/66‑148 (MQ=255)
                                                                       aaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                                   <  8:35055‑M2/83‑1 (MQ=255)
                                                                        aacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGacac                                                                <  8:300364‑M2/86‑1 (MQ=255)
                                                                          catgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACtt                                                              >  3:365776‑M2/62‑149 (MQ=255)
                                                                           atgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTg                                                             >  4:282656‑M2/61‑149 (MQ=255)
                                                                            tgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGcc                                                                                                                      <  6:290430‑M2/32‑1 (MQ=255)
                                                                            tgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGcc                                                                                                                      >  5:290430‑M2/60‑91 (MQ=255)
                                                                            tgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTgc                                                            <  5:158750‑M2/90‑1 (MQ=255)
                                                                                    tgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctc                                                    >  7:6200‑M2/52‑149 (MQ=255)
                                                                                    tgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctc                                                    >  3:317717‑M2/52‑149 (MQ=255)
                                                                                      aaatgtctgacgcagccgagaagaatatccccgcttaatcgcaccttccTTAATTTCTTTACTTTCCGCTGATGAGGAGCCAGACTGAAATCAGCCTATAGGAGTAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCtctctc                                                  <  8:146259‑M2/100‑1 (MQ=255)
                                                                                      aaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCtctctc                                                  <  6:391055‑M2/100‑1 (MQ=255)
                                                                                       aatgactgagtcagccgagaagaatttccccgcttattcgcaacttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCtctctc                                                  <  6:64572‑M2/100‑1 (MQ=255)
                                                                                        atgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                <  8:55609‑M2/102‑1 (MQ=255)
                                                                                           actgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATa                                              <  2:292607‑M2/104‑1 (MQ=255)
                                                                                             tgagtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctct                                                   >  1:117054‑M2/43‑141 (MQ=255)
                                                                                             tgagtcagccgagaagaatttccccgcttattcgcaccttacTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctct                                                   <  2:117054‑M2/99‑1 (MQ=255)
                                                                                               agtcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCAGATTCGGTGCCAGACTGAAATCAGACTATAGGATGAAATGATGGTACGTATCTATACGTTGCCACTTGCGCCCTCTCTCGATAgcgc                                          >  6:187894‑M2/41‑148 (MQ=255)
                                                                                                 tcagccgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACAGAAATCAGCCTAAAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCTCCCACTATCGATAGCGcaa                                        >  8:347872‑M2/39‑148 (MQ=255)
                                                                                                      cgagaagaatttccccgctttttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTa                                  <  7:347872‑M2/116‑1 (MQ=255)
                                                                                                      cgagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTa                                  <  1:399586‑M2/116‑1 (MQ=255)
                                                                                                       gagaagaatttccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAc                                 >  8:387000‑M2/33‑149 (MQ=255)
                                                                                                                  ccccgcttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAtt                       >  1:328211‑M2/22‑148 (MQ=255)
                                                                                                                        ttattcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATccc                 >  2:328567‑M2/16‑148 (MQ=255)
                                                                                                                          attcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGa               <  2:328211‑M2/135‑1 (MQ=255)
                                                                                                                           ttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa              <  3:263778‑M2/136‑1 (MQ=255)
                                                                                                                              gcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGa           >  6:310043‑M2/10‑148 (MQ=255)
                                                                                                                               caccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGaa          >  5:195346‑M2/9‑148 (MQ=255)
                                                                                                                                  cttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACt      >  8:118459‑M2/6‑149 (MQ=255)
                                                                                                                                  cttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACt      >  4:274872‑M2/6‑149 (MQ=255)
                                                                                                                                      cTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa              <  5:151669‑M2/136‑1 (MQ=255)
                                                                                                                                      cTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa              >  6:151669‑M2/2‑137 (MQ=255)
                                                                                                                                      cTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTgcgc  >  4:228685‑M2/2‑149 (MQ=255)
                                                                                                                                       ttAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa              >  2:17306/1‑136 (MQ=255)
                                                                                                                                       ttAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGaa              <  1:17306/136‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                         
GAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGC  >  NC_000913/1806174‑1806456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: