Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,120,246 A→G 5.3% Y437H (TAC→CAC)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,120,2460AG 5.3% 235.9 / 3.0 76Y437H (TAC→CAC) wcaJcolanic biosynthesis UDP‑glucose lipid carrier transferase
Reads supporting (aligned to +/- strand):  ref base A (36/36);  new base G (2/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

TGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTA  >  NC_000913/2120098‑2120394
                                                                                                                                                    |                                                                                                                                                    
tgatCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTa                                                                                                                                                      <  2:36136/149‑1 (MQ=255)
    cACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTc                                                                                                                                                   >  7:284366/1‑148 (MQ=255)
    cACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTc                                                                                                                                                   >  6:48660/1‑148 (MQ=255)
     aCCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCaa                                                                                                                                                 <  5:142749/149‑1 (MQ=255)
        gTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGCCGCTCCATTCGCGGATGTACTCAAGGt                                                                                                                                              >  2:47710/1‑149 (MQ=255)
                  ccGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCa                                                                                                                                                  >  3:349632/1‑135 (MQ=255)
                  ccGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCa                                                                                                                                                  <  4:349632/135‑1 (MQ=255)
                    gaacACTTCGCGCCGCTGATGGTTTTTTAACGTAAGCTCATATAAATATGCCGCTTTGTTAACGAAAGCTTTGAACACAGTCAGGAAAACGATTATGATATCGAACCAGCAGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGActc                                                                                                                                  >  6:243795/4‑147 (MQ=255)
                    gACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGAcgc                                                                                                                                  >  2:68755/1‑149 (MQ=255)
                               gccgcTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCAtt                                                                                                                       <  4:136561/149‑1 (MQ=255)
                               gccgcTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCAtt                                                                                                                       <  1:282289/149‑1 (MQ=255)
                                      atttttttttCAACTAATCACATATCAATATTCCGCTTTGTTAACGAAAACTATGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTtctc                                                                                                                  <  8:150555/143‑1 (MQ=255)
                                      aTGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCTAACTCGACGCGTTTTTCCATTTtctc                                                                                                                  <  1:95027/147‑1 (MQ=255)
                                      aTGGTGTTTTCACGTAAGCTCATTTCAATATGGCGCTTTGTTATCGAAACCTTTGAACACCTTCAGGTTAACGATTTTGTTCTCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTtctc                                                                                                                  <  8:203309/147‑1 (MQ=255)
                                         gTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCatc                                                                                                              <  2:10733/148‑3 (MQ=255)
                                          ttttttCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCgt                                                                                                            >  6:44021/1‑149 (MQ=255)
                                                cACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGt                                                                                                      >  7:211082/1‑149 (MQ=255)
                                                 aCGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGACCACAGTCAGTAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCACGGTCGAACTCGAGTCGTTTATCCATTTTCTCCAGCGTGTCGGtt                                                                                                     >  6:286983/1‑149 (MQ=255)
                                                 aCGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGtt                                                                                                     >  1:339187/1‑149 (MQ=255)
                                                  cGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGttt                                                                                                    <  5:48660/149‑1 (MQ=255)
                                                   gTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGtttt                                                                                                   >  2:147619/1‑148 (MQ=255)
                                                           atatCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCgcgc                                                                                            >  4:53330/1‑148 (MQ=255)
                                                             atCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAg                                                                                         >  2:236149/1‑149 (MQ=255)
                                                             atCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAg                                                                                         >  1:114849/1‑149 (MQ=255)
                                                               cAATATGCCGGTTTGTTTACGAAACCTTTGTACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGc                                                                                        <  7:58420/148‑1 (MQ=255)
                                                                 atatGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGt                                                                                     <  1:368909/149‑1 (MQ=255)
                                                                   atGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGtt                                                                                    >  2:343520/1‑148 (MQ=255)
                                                                     gCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAAt                                                                                 >  8:102079/1‑149 (MQ=255)
                                                                             gTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGcc                                                                          >  5:180903/1‑148 (MQ=255)
                                                                              ttAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTgc                                                                             >  8:265490/1‑144 (MQ=255)
                                                                              ttAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTgc                                                                             <  7:265490/144‑1 (MQ=255)
                                                                              ttAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGcc                                                                          >  2:121578/1‑147 (MQ=255)
                                                                                aaCGAAACCTTTGAACACCGTCAGGAATACGATTTTGATATCGAACCACACGCTCCATTCGCGGATGTACTCAAGGTCCAACTCGACGCGTTTTTCCATTTTCTCCAGAGTGTCCGTTTCTCCGCGCCAGCCGTTAATCTGCGCCCAGc                                                                      >  4:96194/1‑149 (MQ=255)
                                                                                 aCGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGcc                                                                     >  7:390774/1‑149 (MQ=255)
                                                                                  cGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATGTTCTCCCGCGTGTCGGGTTCGCCGCGCCAGCCGTTAAACTGCGCCCAGCCg                                                                    >  4:155114/1‑149 (MQ=255)
                                                                                   gAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTgcgc                                                                           >  6:382935/1‑141 (MQ=255)
                                                                                   gAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTgcgc                                                                           <  5:382935/141‑1 (MQ=255)
                                                                                    aaaCCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCgg                                                                   >  3:149369/1‑148 (MQ=255)
                                                                                        cTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATg                                                              >  3:251911/1‑149 (MQ=255)
                                                                                             aaCACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCg                                                          <  1:68755/148‑1 (MQ=255)
                                                                                             aaCACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGGCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCgg                                                         <  5:228969/149‑1 (MQ=255)
                                                                                              acacCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCgg                                                         <  4:251911/148‑1 (MQ=255)
                                                                                               cacCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTGCTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAAt                                                                                 >  4:297279/1‑123 (MQ=255)
                                                                                               cacCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTGCTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAAt                                                                                 <  3:302378/123‑1 (MQ=255)
                                                                                               cacCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTGCTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAAt                                                                                 >  4:302378/1‑123 (MQ=255)
                                                                                               cacCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTGCTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAAt                                                                                 <  3:297279/123‑1 (MQ=255)
                                                                                                  cGTCAGGAAAACCATTTTTACACCGAACCAGACGCTCCCTTCGCGCAGTTACTAAAGGGCGAACTCGACGCGTTTTTACATTTTCTTCAGGGTTGCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCa                                                    <  8:109480/149‑1 (MQ=255)
                                                                                                    tCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGACGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCAcc                                                  >  3:363169/1‑149 (MQ=255)
                                                                                                         aaaaCGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTTGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATg                                             <  1:147619/149‑1 (MQ=255)
                                                                                                         aaaaCGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATg                                             <  3:53330/149‑1 (MQ=255)
                                                                                                         aaaaCGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATg                                             <  8:211082/149‑1 (MQ=255)
                                                                                                         aaaaCGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATg                                             <  1:121578/149‑1 (MQ=255)
                                                                                                         aaaaCCATTTTGATTTCGAAACAGACGCGCCAATCCCGGATGTACTCAATTTCGAACTCTACGTTTTTTTCCATTATCTCCAGCGTGTCTGATTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATg                                             <  4:4680/149‑1 (MQ=255)
                                                                                                          aaaCGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACTCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCTGTAATGCCCGGTTTCACCTTATgg                                            >  5:181768/1‑149 (MQ=255)
                                                                                                          aaaCGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCTCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATgg                                            <  7:47497/149‑1 (MQ=255)
                                                                                                            aCGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGgcg                                          <  6:180903/149‑1 (MQ=255)
                                                                                                             cGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGgcgc                                         <  1:343520/149‑1 (MQ=255)
                                                                                                              gATTTTGATCTCGATCCAGACGCTCCATTCGCGGATGTACTCTAGGTCGACCTCGCCGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCGGCGCCAGCCGTTAATCTGCGCCCCGCCGGAAATGCCCGGTTTCACCTTATGGCgca                                        <  5:10954/149‑1 (MQ=255)
                                                                                                                ttttGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCTAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCgcag                                       >  1:162972/1‑148 (MQ=255)
                                                                                                                     atatCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTa                                 <  2:339187/149‑1 (MQ=255)
                                                                                                                        tCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGc                               <  7:102079/148‑1 (MQ=255)
                                                                                                                        tCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGCTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGcc                              >  8:367957/1‑149 (MQ=255)
                                                                                                                          cagccAGACGATCCATTCGCGGAGGTACTCAATGTCGAACTCGACGCGTTTTTCCATAATCTCCAGCGTGACGGTTTCGCCGCGCCCTCCGTTAATCTGCGACAAGCCCGAAATGCCCGGGTTCACCTGATGGCGCAGCATGTAGCCtt                            >  4:242646/4‑149 (MQ=255)
                                                                                                                             ccAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCaa                         <  2:33839/149‑1 (MQ=255)
                                                                                                                              cAGCCGCTCCATTCGCGGATGTACTCACGGTGGAACTCGACGCGGTTTTCCATTTTCTCCTGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAAt                        <  8:44358/149‑1 (MQ=255)
                                                                                                                              cAGACGCTACATTCGCGGATGTACTCAAGGTCGAACTCGCCGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAAt                        >  5:330995/1‑149 (MQ=255)
                                                                                                                                gACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCgg                                                                   >  7:270651/1‑104 (MQ=255)
                                                                                                                                gACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCgg                                                                   <  8:270651/104‑1 (MQ=255)
                                                                                                                                gACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCa                                                    >  4:320083/1‑119 (MQ=255)
                                                                                                                                gACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCa                                                    <  3:320083/119‑1 (MQ=255)
                                                                                                                                 aCGCTCCATTCGCGGAGGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAg                     <  1:47710/149‑1 (MQ=255)
                                                                                                                                 aCGCTCCAGTCGAGGCTGTACTCACGGTCGAACTCGACGCGTTTTTCCATTTTCTGCAGCGTGTCGGTTGCGCCGCGCCAGCCGTTACTCTGCGCCCAGCCGGAAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAg                     <  5:243795/149‑1 (MQ=255)
                                                                                                                                    cTCCATTCGCGGATGTACTCAAGGTCTAACTAGACGGGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCAAGCAGGTAATGCCCGGTTTCACCTTATGGAGCAGCATGTAGCCTTCAATGAGCTg                  >  1:259672/1‑149 (MQ=255)
                                                                                                                                       cATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTc                <  5:44021/148‑1 (MQ=255)
                                                                                                                                         ttCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGa              >  1:27802/1‑148 (MQ=255)
                                                                                                                                              ggATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGt        <  8:390774/149‑1 (MQ=255)
                                                                                                                                                  gTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGt    >  2:273496/1‑149 (MQ=255)
                                                                                                                                                    aCTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTa  >  2:249749/1‑149 (MQ=255)
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TGATCACCGTGGCAATCGCCGACCACTTCGCGCCGCTGATGGTTTTTTCACGTAAGCTCATATCAATATGCCGCTTTGTTAACGAAACCTTTGAACACCGTCAGGAAAACGATTTTGATATCGAACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTA  >  NC_000913/2120098‑2120394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: