Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,398,261 A→T 5.3% D635E (GAT→GAA nuoG ← NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,398,2610AT 5.3% 245.1 / 3.0 76D635E (GAT→GAAnuoGNADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand):  ref base A (38/34);  new base T (2/2);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

TTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAACCACCATCACCAGCGGTGCT  >  NC_000913/2398120‑2398408
                                                                                                                                             |                                                                                                                                                   
ttCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATAACAGTTTTGCTGTCGTAATAAGCAGGATCGTaaa                                                                                                                                              <  6:17543/149‑1 (MQ=255)
   cGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAACCCt                                                                                                                                           >  1:288154/1‑149 (MQ=255)
   cGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCt                                                                                                                                           >  6:188632/1‑149 (MQ=255)
     ggATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGTTCGTAAACCTg                                                                                                                                          >  1:160895/1‑148 (MQ=255)
        ttttCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGgaag                                                                                                                                      <  3:325341/149‑1 (MQ=255)
          ttCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGgaaga                                                                                                                                     <  5:188632/148‑1 (MQ=255)
               cacaACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGtt                                                                                                                               >  3:124747/1‑149 (MQ=255)
                 caacaGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTgg                                                                                                                             <  7:267782/149‑1 (MQ=255)
                  aacaGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTggg                                                                                                                            >  3:157710/1‑149 (MQ=255)
                       cGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCgg                                                                                                                       >  3:249267/1‑149 (MQ=255)
                               acaTGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCgtt                                                                                                               >  5:136520/1‑149 (MQ=255)
                                caTGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCgttg                                                                                                              <  6:38886/149‑1 (MQ=255)
                                 aTGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCgttgt                                                                                                             <  4:249267/149‑1 (MQ=255)
                                 aTGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCgttgt                                                                                                             >  2:23461/1‑149 (MQ=255)
                                  tGGTCGAGCTGCGTGCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGTTCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCgttgtt                                                                                                            >  3:195166/1‑149 (MQ=255)
                                  tGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCgttgtt                                                                                                            <  5:182292/149‑1 (MQ=255)
                                  tGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCgttgtt                                                                                                            <  5:160305/149‑1 (MQ=255)
                                   ggTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTCCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCgttgtt                                                                                                            >  5:78596/1‑148 (MQ=255)
                                             cGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGTTCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCaccgt                                                                                                   <  4:195166/147‑1 (MQ=255)
                                                    tccaCTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCt                                                                                          >  1:242724/1‑149 (MQ=255)
                                                       aCTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGAt                                                                                                         <  4:223524/131‑1 (MQ=255)
                                                       aCTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGAt                                                                                                         >  3:223524/1‑131 (MQ=255)
                                                         ttCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAg                                                                                     >  3:16342/1‑149 (MQ=255)
                                                         ttCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAg                                                                                     >  8:21480/1‑149 (MQ=255)
                                                          tCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAg                                                                                     >  6:6486/1‑148 (MQ=255)
                                                           cACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGc                                                                                    >  4:138143/1‑148 (MQ=255)
                                                              ggCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAg                                                                                >  7:259607/1‑149 (MQ=255)
                                                                   agcagGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGTTCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTgg                                                                            <  2:160895/148‑1 (MQ=255)
                                                                   agcagGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTgg                                                                            >  5:30179/1‑148 (MQ=255)
                                                                    gcagGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTgg                                                                            >  4:317326/1‑147 (MQ=255)
                                                                     cagGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGgcag                                                                         >  5:288142/1‑149 (MQ=255)
                                                                     cagGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGgcag                                                                         <  4:270373/149‑1 (MQ=255)
                                                                            cTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGaaa                                                                   <  1:132965/148‑1 (MQ=255)
                                                                            cTGTGCAGCGAGTGTAACCAGCGCCAGCATTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAg                                                                  >  1:107313/1‑149 (MQ=255)
                                                                              gtgCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTa                                                                <  8:299651/149‑1 (MQ=255)
                                                                               tgCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTAc                                                               <  6:30179/149‑1 (MQ=255)
                                                                                   gCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTg                                                                             >  7:248007/1‑131 (MQ=255)
                                                                                   gCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTg                                                                             <  8:248007/131‑1 (MQ=255)
                                                                                   gCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTAc                                                               >  7:339445/1‑145 (MQ=255)
                                                                                   gCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTAc                                                               >  4:216637/1‑145 (MQ=255)
                                                                                   gCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTAc                                                               >  4:33507/1‑145 (MQ=255)
                                                                                   gCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGGTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGa                                                           <  1:53505/149‑1 (MQ=255)
                                                                                       gtgtAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATggg                                                       >  4:258222/1‑149 (MQ=255)
                                                                                           aaCCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCa                                                                                      >  2:133306/1‑114 (MQ=255)
                                                                                           aaCCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCa                                                                                      <  1:133306/114‑1 (MQ=255)
                                                                                             ccAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGtttt                                                 <  1:23461/149‑1 (MQ=255)
                                                                                               aGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTcc                                               <  3:258222/149‑1 (MQ=255)
                                                                                               aGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTc                                                >  6:187044/1‑148 (MQ=255)
                                                                                                gcgcCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCa                                              <  5:56911/149‑1 (MQ=255)
                                                                                                  gcCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCAt                                             >  5:49874/1‑148 (MQ=255)
                                                                                                     aGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATAGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATc                                         <  2:308304/149‑1 (MQ=255)
                                                                                                          tCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCtgt                                    >  8:341239/1‑149 (MQ=255)
                                                                                                          tCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCt                                      <  8:317667/147‑1 (MQ=255)
                                                                                                           ccAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCtgt                                    <  7:21480/148‑1 (MQ=255)
                                                                                                              gCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGt                                >  1:219950/1‑149 (MQ=255)
                                                                                                                aTGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTtg                              <  3:317326/149‑1 (MQ=255)
                                                                                                                  gACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTtgat                            >  8:37021/1‑149 (MQ=255)
                                                                                                                  gACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTtgat                            >  6:194001/1‑149 (MQ=255)
                                                                                                                   aCAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTtgatg                           <  6:136520/149‑1 (MQ=255)
                                                                                                                    cAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTtgatga                          >  8:265293/1‑149 (MQ=255)
                                                                                                                     aGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCGATAATCGCTGTGCGTTgatgat                         >  4:237404/1‑149 (MQ=255)
                                                                                                                     aGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTgatgat                         <  2:278592/149‑1 (MQ=255)
                                                                                                                      gTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATc                        <  8:339445/149‑1 (MQ=255)
                                                                                                                           gCTGTAGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAacca                   >  1:70266/1‑149 (MQ=255)
                                                                                                                            cTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAaccac                  <  3:138143/149‑1 (MQ=255)
                                                                                                                             tGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAaccac                  >  7:44781/1‑148 (MQ=255)
                                                                                                                             tGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAaccac                  >  6:216267/1‑148 (MQ=255)
                                                                                                                               tCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAaccacc                 <  6:288142/147‑1 (MQ=255)
                                                                                                                               tCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAAccacca                <  5:187044/148‑1 (MQ=255)
                                                                                                                                 gTAATAAGCAGGATCGTAAATCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTCCCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAACCACCATc              <  2:107572/148‑1 (MQ=255)
                                                                                                                                 gTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAACCACCATCa             <  5:194001/149‑1 (MQ=255)
                                                                                                                                         cAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAACCACCATCACCAGCGGt     <  3:259205/149‑1 (MQ=255)
                                                                                                                                         cAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAACCACCATCACCAGCGGt     <  8:259607/149‑1 (MQ=255)
                                                                                                                                          aGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAACCACCATCACCAGCGGTg    >  1:118949/1‑149 (MQ=255)
                                                                                                                                          aGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAACCACCATCACCAGCGGTg    <  6:49874/149‑1 (MQ=255)
                                                                                                                                            gATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAACCACCATCACCAGCGGTGCt  >  7:2400/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
TTCCGGGATTTTCGCCACAACAGCGTCAATCACATGGTCGAGCTGCGTCCAGTCCACTTCACGGCTCAGCAGGGTGCTGTGCAGCGAGTGTAACCAGCGCCAGCTTTCCAGCATGACAGTTTTGCTGTCGTAATAAGCAGGATCGTAAACCTGGAAGAAACGTTGGGCGCGGCCTTCGTTGTTGATCACCGTACCGTCGCTTTCAGCAAAGCTGGCAGCAGAAAGTACCAGATGGGCGTTTTCCATAATCGCTGTGCGTTGATGATCAACCACCATCACCAGCGGTGCT  >  NC_000913/2398120‑2398408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: