Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,982	G→A	V205I (GTC→ATC)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,982	0	G	A	100.0%	324.2 / NA	92	V205I (GTC→ATC)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/50);  total (42/50)

AACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAG  >  NC_000913/1806842‑1807118                                                                                                                                             |                                                                                                                                         aaCAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCaaa                                                                                                                                  <  7:68056/149‑1 (MQ=255) aaCAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGGTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCaaa                                                                                                                                  <  7:421465/149‑1 (MQ=255)      gggATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCcgc                                                                                                                             >  3:190939/1‑149 (MQ=255)       ggATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCcgcg                                                                                                                            >  3:111253/1‑149 (MQ=255)         aTCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCa                                                                                                                          <  8:194981/149‑1 (MQ=255)            cGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAgg                                                                                                                        >  2:157002/1‑148 (MQ=255)                 cATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTATCCAAAAAGAACCCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCg                                                                                                                  <  1:433165/149‑1 (MQ=255)                 cATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAAt                                                                                                                    <  7:340221/147‑1 (MQ=255)                    cgtcgACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGtt                                                                                                                >  6:147530/1‑148 (MQ=255)                     gtcgACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTaa                                                                                                              >  7:249516/1‑149 (MQ=255)                        gACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACCTCGAGTTGGTTAAGCCTAACCAAAAAGACCTCAGTGCGCTGGTGAATCCCGAACTCACCCAGCCGGACGATATACGCAACGCCGAGCATGAAATCGTTAATAg                                                                                                           >  5:128965/1‑149 (MQ=255)                         aCAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGc                                                                                                          >  7:424376/1‑149 (MQ=255)                          cAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCg                                                                                                         <  4:111253/149‑1 (MQ=255)                            gTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCgg                                                                                                        >  2:327776/1‑148 (MQ=255)                             ttCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGc                                                                                                       >  4:201168/1‑148 (MQ=255)                                  gCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAgg                                                                                                   <  6:418120/147‑1 (MQ=255)                                  gCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAgg                                                                                                   <  3:96422/147‑1 (MQ=255)                                  gCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAg                                                                                                    <  5:369116/146‑1 (MQ=255)                                  gCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAg                                                                                                    <  8:262534/146‑1 (MQ=255)                                  gCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCACGCAGGAAATCGTTAATAGCGGCAAg                                                                                                    <  8:129842/146‑1 (MQ=255)                                      aGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAAc                                                                                             <  1:157002/149‑1 (MQ=255)                                      aGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAAc                                                                                             <  3:6710/149‑1 (MQ=255)                                              gTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTc                                                                                     <  3:201168/149‑1 (MQ=255)                                                  agcaCTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTTGTGAATCGCGAACTCACCCAGCCGGACGATATCCTCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGttt                                                                                 >  5:71096/1‑149 (MQ=255)                                                   gcaCTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTc                                                                                >  1:183380/1‑149 (MQ=255)                                                      cTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCt                                                                             <  6:416580/149‑1 (MQ=255)                                                       tGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTg                                                                            >  4:296075/1‑149 (MQ=255)                                                       tGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTg                                                                            >  1:32089/1‑149 (MQ=255)                                                          cAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGt                                                                         <  2:183380/149‑1 (MQ=255)                                                            aTTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTcc                                                                       <  2:32089/149‑1 (MQ=255)                                                                   aCATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAgg                                                                 <  3:401949/148‑1 (MQ=255)                                                                   aCATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAg                                                                  >  2:157120/1‑147 (MQ=255)                                                                   aCATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCCCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGa                                                                <  6:391247/149‑1 (MQ=255)                                                                    cATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGc                                                                                                  <  8:280523/114‑1 (MQ=255)                                                                    cATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAg                                                               >  7:307783/1‑149 (MQ=255)                                                                     aTCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTc                                                                                     <  4:117802/126‑1 (MQ=255)                                                                     aTCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTc                                                                                     >  3:117802/1‑126 (MQ=255)                                                                     aTCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAgc                                                              >  3:57299/1‑149 (MQ=255)                                                                      tCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAgcg                                                             >  4:174106/1‑149 (MQ=255)                                                                      tCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAgcg                                                             <  8:249516/149‑1 (MQ=255)                                                                        gAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGa                                                                >  4:182607/1‑144 (MQ=255)                                                                        gAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGa                                                                <  3:182607/144‑1 (MQ=255)                                                                        gAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCt                                                           >  7:76382/1‑149 (MQ=255)                                                                        gAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCt                                                           >  8:384911/1‑149 (MQ=255)                                                                         aGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTg                                                          >  4:121563/1‑149 (MQ=255)                                                                          gTTGGTTTAGACTAACCAAAAAGAACTCAGTGCTCTGGTGATTCGCGAACTCACCCAGCCGGACGATATCCGCAACGCCGAGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTgg                                                         <  6:128965/149‑1 (MQ=255)                                                                          gTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTgg                                                         <  7:384911/149‑1 (MQ=255)                                                                             ggTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGgt                                                       <  3:296075/148‑1 (MQ=255)                                                                              gTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTggg                                                                          <  5:422635/128‑1 (MQ=255)                                                                              gTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTggg                                                                          >  6:422635/1‑128 (MQ=255)                                                                              gTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGgtg                                                      <  8:351386/148‑1 (MQ=255)                                                                              gTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGgtg                                                      <  2:232233/148‑1 (MQ=255)                                                                               ttAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGtt                                                    <  4:190939/149‑1 (MQ=255)                                                                                     cTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGt                                              >  8:219981/1‑149 (MQ=255)                                                                                        aCCAAAAAGAACTCAGTGCGCTGGTTAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTg                                             <  8:248179/147‑1 (MQ=255)                                                                                        aCCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGa                                            >  7:103985/1‑148 (MQ=255)                                                                                         ccAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAAc                                                                                             <  1:7512/98‑1 (MQ=255)                                                                                          cAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaaaa                                         >  1:416161/1‑149 (MQ=255)                                                                                           aaaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAAc                                        >  5:138924/1‑149 (MQ=255)                                                                                           aaaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAAc                                        >  7:261713/1‑149 (MQ=255)                                                                                            aaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTgg                                                         >  3:193539/1‑131 (MQ=255)                                                                                            aaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTgg                                                         <  4:193539/131‑1 (MQ=255)                                                                                                   cTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGgtgt                                                     >  4:352933/1‑128 (MQ=255)                                                                                                   cTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGgtgt                                                     <  3:352933/128‑1 (MQ=255)                                                                                                    tCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAg                                                                  >  4:76075/1‑114 (MQ=255)                                                                                                    tCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAg                                                                  <  3:76075/114‑1 (MQ=255)                                                                                                        tGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAggtgg                           <  1:327776/149‑1 (MQ=255)                                                                                                          cgcTGGTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAggtggt                          <  3:174106/148‑1 (MQ=255)                                                                                                               gTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTc                                                                        <  1:173452/97‑1 (MQ=255)                                                                                                               gTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTc                                                                        >  2:173452/1‑97 (MQ=255)                                                                                                               gTGAATCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGccacc                    >  8:189644/1‑149 (MQ=255)                                                                                                                  aaTCGCGAACTCATCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTgg                                                                           >  2:33623/1‑91 (MQ=255)                                                                                                                  aaTCGCGAACTCATCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTgg                                                                           <  1:33623/91‑1 (MQ=255)                                                                                                                    tCGCGAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCgg               >  7:180229/1‑149 (MQ=255)                                                                                                                      gcgAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGGCaccacctgaa             <  1:157120/149‑5 (MQ=255)                                                                                                                      gcgAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGt              >  6:80539/1‑148 (MQ=255)                                                                                                                      gcgAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGt              >  1:384995/1‑148 (MQ=255)                                                                                                                      gcgAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGt              <  4:57299/148‑1 (MQ=255)                                                                                                                        gAACTCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGaa           <  5:147530/149‑1 (MQ=255)                                                                                                                            tCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaaaa                                         <  6:342152/115‑1 (MQ=255)                                                                                                                            tCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGaaaa                                         >  5:342152/1‑115 (MQ=255)                                                                                                                            tCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAggtg                            <  4:345292/128‑1 (MQ=255)                                                                                                                            tCACCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGAGTTGATAGTGAAAACTGTATTCAggtg                            >  3:345292/1‑128 (MQ=255)                                                                                                                              aCCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGTGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCa     <  8:76382/149‑1 (MQ=255)                                                                                                                              aCCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCa     <  8:307783/149‑1 (MQ=255)                                                                                                                              aCCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCa     <  3:121563/149‑1 (MQ=255)                                                                                                                              aCCCAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCa     <  7:189644/149‑1 (MQ=255)                                                                                                                                ccAGCCGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCa     >  4:271833/1‑147 (MQ=255)                                                                                                                                    ccGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCagag  >  1:156951/1‑146 (MQ=255)                                                                                                                                    ccGGACGATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCagag  <  2:156951/146‑1 (MQ=255)                                                                                                                                          gATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGt              >  8:286542/1‑128 (MQ=255)                                                                                                                                          gATATCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGt              <  7:286542/128‑1 (MQ=255)                                                                                                                                             |                                                                                                                                         AACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAG  >  NC_000913/1806842‑1807118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑