New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4195732 =93 (0.980)3 (0.030) 3/246 NT 3.6% coding (368/1896 nt) thiC phosphomethylpyrimidine synthase
?NC_000913 4195811 = 74 (0.830)coding (289/1896 nt) thiC phosphomethylpyrimidine synthase
Rejected: Frequency below/above cutoff threshold.

GGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4195884‑4195732
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggctggcGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTC  >  NC_000913/4195811‑4195958
                                                                                                                                                                                                                                                                                                             
GGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGC                                                                                                                                                          >  3:356308/1‑149
  TCTACGACACCTCCGGCCCGTATGGTGATCCGGAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTTAAGCGCGGCTG                                                                                                                                                        <  5:141842/149‑1
  TCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTG                                                                                                                                                        >  5:283009/1‑149
    TACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGG                                                                                                                                                       >  1:10053/1‑148
      CGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGC                                                                                                                                                      <  4:174856/147‑1
      CGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGC                                                                                                                                                      <  4:353468/147‑1
          ACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGG                                                                                                                                                           <  3:284867/138‑1
          ACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGG                                                                                                                                                           >  4:284867/1‑138
               CGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCT                                                                                                                                                         >  8:235219/1‑135
               CGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCT                                                                                                                                                         <  7:235219/135‑1
                                                                            CAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCCTACGGGCCGGAGGTGTCGTAGA                                                                              >  6:268189/1‑149
                                                                                 GTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGG                                                                          >  3:313598/1‑148
                                                                                         ATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTC                                                                 <  4:313598/149‑1
                                                                                                                                                       GCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCT   >  3:157096/1‑149
                                                                                                                                                       GCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATC    >  6:179584/1‑148
                                                                                                                                                       GCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGAT     <  4:380984/147‑1
                                                                                                                                                        CGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTC  <  5:195583/149‑1
                                                                                                                                                                                                                                                                                                             
GGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4195884‑4195732
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggctggcGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTC  >  NC_000913/4195811‑4195958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.